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    THERMAL CALIBRATION
    2.
    发明申请
    THERMAL CALIBRATION 审中-公开
    热校准

    公开(公告)号：US20120178077A1

    公开(公告)日：2012-07-12

    申请号：US13223258

    申请日：2011-08-31

    申请人： Fernando DeCastro ,  Renee Howell ,  Sami Kanderian ,  Johnathan S. Coursey ,  Kenton C. Hasson ,  Scott Sundberg

    发明人： Fernando DeCastro ,  Renee Howell ,  Sami Kanderian ,  Johnathan S. Coursey ,  Kenton C. Hasson ,  Scott Sundberg

    IPC分类号： G01N25/04 ,  C09K3/00 ,  G01K15/00 ,  C07H21/04

    CPC分类号： G01K11/06 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2300/1827 ,  C12Q1/6806 ,  G01K15/00 ,  C12Q2527/107 ,  C12Q2531/113 ,  C12Q2545/101 ,  C12Q2545/113 ,  C12Q2545/114 ,  C12Q2565/629

    摘要： The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.

    摘要翻译： 本发明涉及一种或多种扩增子作为温度校准剂的用途。 在一些实施方案中，校准物可用于校准其中进行扩增和/或熔融分析的微流体通道的温度。 在一些实施方案中，扩增子可以是基因组，超保守元件和/或合成的。 扩增子可以具有已知或预期的熔融温度。 校准物可以加入到研究的引物中，或者可以跟踪或引导通道中研究的引物。 可以扩增和融合扩增子，并且可以确定扩增子熔化的温度。 测量的温度可以与扩增子预期熔化的已知温度进行比较。 测量温度和预期温度之间的差异可用于校准/调节用于控制和/或检测通道温度的一个或多个温度控制元件。

    Process for the fractionation of cereal brans
    4.
    发明申请
    Process for the fractionation of cereal brans 有权
    麦芽糊精分馏的方法

    公开(公告)号：US20050089602A1

    公开(公告)日：2005-04-28

    申请号：US10643402

    申请日：2003-08-19

    申请人： Sten Kvist ,  Tommie Carlsson ,  John Lawther ,  Fernando DeCastro

    发明人： Sten Kvist ,  Tommie Carlsson ,  John Lawther ,  Fernando DeCastro

    IPC分类号： A23D9/00 ,  A23J1/14 ,  A23L1/10 ,  C11B1/02 ,  C11B1/10 ,  A23K1/00

    CPC分类号： C11B1/025 ,  A23D9/00 ,  A23J1/14 ,  A23L7/115 ,  C11B1/10 ,  C11B1/104

    摘要： A process for the fractionation of valuable fractions from cereal brans (e.g. wheat, barley and oat brans, and rice polish) is described. In particular, this invention describes a two step process, in which the said bran is first subjected to a combination of enzymatic treatment and wet milling, followed by sequential centrifugation and ultrafiltration, which aims at physically separating the main bran factions, i.e. insoluble phase (pericarp and aleurone layer), germ-rich fraction, residual endosperm fraction and soluble sugars. A second step consists of fractionating cereal brans substantially free of soluble compounds, hence insoluble phase from the above-mentioned first step, by enzymatic treatment with xylanases and/or beta-glucanase and wet milling, followed by sequential centrifugation and ultrafiltration, which aims at physically separating the main fractions, i.e. insoluble phase (remaining cell wall components), protein-rich fraction, soluble hemicellulose and oligosaccharide, and therefore maximizes the extraction rate of valuable cell wall components and aleurone cells from previously cleaned bran.

    摘要翻译： 描述了从谷物（例如小麦，大麦和燕麦，米糠）中分离出有价值馏分的方法。 特别地，本发明描述了两步法，其中首先对所述麸进行酶处理和湿法研磨的组合，然后进行连续离心和超滤，其目的是物理分离主要的麸皮部分，即不溶相（ 果皮和糊粉层），富含胚芽的部分，残留的胚乳部分和可溶性糖。 第二步是通过用木聚糖酶和/或β-葡聚糖酶进行酶处理和湿磨，然后进行连续离心和超滤，将基本上不含可溶性化合物的麦芽糖，因此不溶于上述第一步的不溶相， 物理分离主要级分，即不溶相（剩余细胞壁组分），富含蛋白质的级分，可溶性半纤维素和寡糖，因此使来自先前清洁的麸皮的有价值的细胞壁组分和糊粉母细胞的提取率最大化。