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公开(公告)号：US12124020B2
公开(公告)日：2024-10-22
申请号：US17274295
申请日：2019-09-10
申请人： Fluidigm Canada Inc.
CPC分类号： G02B21/245 , G01N1/04 , G01N33/543 , G02B21/0032 , G02B21/006 , G02B21/26 , H01J49/0004 , H01J49/0031 , H01J49/105 , G01N2001/045 , G01N2458/15
摘要： We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.
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公开(公告)号：US09632079B2
公开(公告)日：2017-04-25
申请号：US14894287
申请日：2014-05-22
申请人： FLUIDIGM CANADA INC.
发明人： Dmitry R. Bandura , Vladimir I. Baranov , Alexander V. Loboda , Olga Ornatsky , Scott D. Tanner
IPC分类号： G01N33/532 , G01N15/10 , H01J49/00 , G01N33/543 , G01N27/62 , G01N33/483 , G01N15/14
CPC分类号： G01N33/54366 , G01N15/1031 , G01N15/1404 , G01N15/1459 , G01N27/622 , G01N27/624 , G01N33/483 , G01N33/532 , G01N33/54313 , G01N2015/1006 , G01N2458/15
摘要： The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
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公开(公告)号：US11264221B2
公开(公告)日：2022-03-01
申请号：US17253538
申请日：2019-06-18
申请人： Fluidigm Canada Inc. , Paul Corkum
发明人： Paul Corkum , Alexander V. Loboda
IPC分类号： H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/40 , H01J49/16 , H01J49/14
摘要： The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US20180312794A1
公开(公告)日：2018-11-01
申请号：US15966432
申请日：2018-04-30
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda
CPC分类号： C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
摘要： Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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公开(公告)号：US20160195466A1
公开(公告)日：2016-07-07
申请号：US14983463
申请日：2015-12-29
申请人： Fluidigm Canada Inc.
CPC分类号： G01N15/1056 , G01N2015/0065 , G01N2015/1006 , H01J49/0009 , H01J49/025 , H01J49/04 , H01J49/105 , H01J49/40
摘要： The inventors have improved mass cytometer to facilitate its use for the analysis of particles.
摘要翻译： 发明人已经改进了质谱仪以便于其用于分析颗粒。
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公开(公告)号：US20160139141A1
公开(公告)日：2016-05-19
申请号：US14940951
申请日：2015-11-13
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/58 , G01N2458/15
摘要： Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.
摘要翻译： 公开了通过使用分子标记的质谱法对生物样品进行成像的方法。
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公开(公告)号：US12000838B2
公开(公告)日：2024-06-04
申请号：US17050716
申请日：2019-04-26
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda , Olga Ornatsky , Vladimir Baranov , Nikita Zabinyakov , Anastasia Mavropoulos , Daniel Majonis , Bedilu Allo , Taunia Closson
CPC分类号： G01N33/6848 , C07K1/13 , G01N1/28 , G01N2458/15 , G01N2560/00
摘要： Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.
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公开(公告)号：US20210404968A1
公开(公告)日：2021-12-30
申请号：US17287923
申请日：2019-10-28
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda , Adam Carew
摘要： We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.
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公开(公告)号：US20210373313A1
公开(公告)日：2021-12-02
申请号：US17274295
申请日：2019-09-10
申请人： Fluidigm Canada Inc.
摘要： We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.
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公开(公告)号：US10472600B2
公开(公告)日：2019-11-12
申请号：US15966432
申请日：2018-04-30
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda
摘要： Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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