公开(公告)号：US20170260573A1

公开(公告)日：2017-09-14

申请号：US15529122

申请日：2015-10-07

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Naoshi ITABASHI ,  Sonoko MIGITAKA ,  Masatoshi NARAHARA ,  Tomohiro SHOJI ,  Yukio ONO

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  B01J19/0046 ,  B01J2219/00421 ,  B01J2219/00466 ,  B01J2219/00621 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B32B27/32 ,  C08G77/50 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: In order to reduce the cost of producing a spot array substrate and reduce the cost of nucleic acid polymer analysis, a spot array substrate is used which is produced by preparing a resin substrate 402 having a surface on which an uneven pattern is formed and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern, and loading surface-modified beads onto the bead sitting positions of the resin substrate.

公开(公告)号：US20160167049A1

公开(公告)日：2016-06-16

申请号：US14907844

申请日：2014-06-11

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Masatoshi NARAHARA ,  Tomohiro SHOJI

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q2565/629

Abstract: Provided is a flow cell for nucleic acid analysis used for a sequence reaction with photocleavable nucleotides where an efficiency of the photocleaving reaction can be enhanced and noise upon florescence detection can be mitigated, thereby improving the accuracy of sequencing, shortening a runtime, and extending a read length. The flow cell for nucleic acid analysis includes a first substrate 101 provided with an optical filter 102 reflecting first light for changing a chemical structure of a substance in a flow passage, a hollow sheet 103 having a hollow portion for forming the flow passage, and a second substrate 105 transmitting the first light, in which the first substrate, the hollow sheet, and the second substrate are attached to each other.

Abstract translation: 提供了用于与可光切割核苷酸的序列反应的核酸分析用流动池，其中可以提高光催化反应的效率，并且可以减轻荧光检测时的噪声，从而提高测序的准确性，缩短运行时间并延长 阅读长度。 用于核酸分析的流动池包括：第一基板101，设置有反射用于改变流路中的物质的化学结构的第一光的滤光器102;具有用于形成流路的中空部的中空片103; 将第一基板，中空板和第二基板彼此附接的第一基板105的第二基板105。

公开(公告)号：US20180017492A1

公开(公告)日：2018-01-18

申请号：US15545422

申请日：2015-02-02

Applicant: Hitachi High-Technologies Corporation

Inventor： Hirokazu KATO ,  Tomohiro SHOJI ,  Satoshi TAKAHASHI ,  Akira MAEKAWA

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01N21/6454 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: A multicolor fluorescence analysis device 1 according to the present invention is provided with: an irradiation unit 41 for irradiating excitation light having a plurality of excitation wavelength bands onto a sample s; a first fiberoptic plate 424 for guiding light including fluorescence emitted from the sample s as a result of the irradiation of the excitation light and emitting the same from a first emission part 425b; a second fiberoptic plate 431 for receiving light emitted from the first emission part 425b at a second incidence part 434a, guiding the same, and emitting the same from a second emission part 434b; a single multilayer dielectric interference film filter 432 that is provided on an end surface of the second emission part 434b, transmits at least a portion of the fluorescence, and transmits light of a plurality of transmission wavelength bands that do not include the excitation wavelength bands; and a two-dimensional detection unit 433 that is disposed so as to be adhered to the multilayer dielectric interference film filter 432 and detect light having passed through the multilayer dielectric interference film filter 432.

公开(公告)号：US20180011021A1

公开(公告)日：2018-01-11

申请号：US15539228

申请日：2015-11-11

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Tomohiro SHOJI ,  Junji SHIOKAWA ,  Akihito NISHIZAWA ,  Hirokazu KATO ,  Junji ISHIZUKA

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01J3/36 ,  G01J3/4406 ,  G01J2003/106 ,  G01N21/0332 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

Abstract: A multicolor fluorescence analysis device 11 is for detecting fluorescence emitted, as a result of excitation light irradiation, from a plurality of types of fluorophores included in a sample s, and is provided with an irradiation optical unit 520 for irradiating light emitted from a light source 510 onto a sample s as excitation light, a fluorescence condensation unit 530 having a fluorescence filter 531 that transmits light emitted from the sample s and transmits light of transmission wavelength bands different from the excitation wavelength bands, and a two-dimensional detector 554 that has a plurality of types of transmission filters 556 for transmitting prescribed wavelengths of light and detects the intensity of the light of the prescribed wavelength for each transmission filter 556, and the light emitted from at least two fluorophores from among the plurality of types of fluorophores is detected simultaneously and the fluorophore types are identified accordingly.

公开(公告)号：US20170089836A1

公开(公告)日：2017-03-30

申请号：US15126781

申请日：2015-03-11

Applicant: Hitachi High-Technologies Corporation

Inventor： Hirokazu KATO ,  Tomohiro SHOJI ,  Hiroyuki HIGASHINO ,  Tatsuya YAMASHITA ,  Mitsuru HARIGAE

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/05

CPC classification number: G01N21/6458 ,  G01N21/0332 ,  G01N21/05 ,  G01N2021/6482

Abstract: An analysis device is provided with a flow chip provided at least with a light transmissive first substrate and a second substrate having an inlet and outlet for a fluid, a holding member for holding the flow chip, a fixing member on which the holding member is placed and that comes into contact with the second substrate of the flow chip, a fluid feeding unit for feeding the fluid to the inlet and discharging the fluid from the outlet, an optical detection unit disposed on the first substrate side of the flow chip, and a drive unit for driving the holding member in the X and Y directions.

公开(公告)号：US20160153960A1

公开(公告)日：2016-06-02

申请号：US14898298

申请日：2014-04-18

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Hirokazu KATO ,  Tomohiro SHOJI

IPC: G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44791 ,  C12Q2565/631

Abstract: By slowing down the passing velocity of a DNA molecule in a nanopore, the accuracy of the reading of a nucleotide sequence of DNA is improved. A small temperature difference is introduced between a DNA molecule having an asymmetric and periodic structure and a nanopore membrane that carries the DNA molecule, whereby the DNA molecule that passes through a nanopore can move in one direction and the passing velocity of the DNA molecule in the nanopore can be controlled and reduced. In this manner, the accuracy of the analysis of a nucleotide sequence can be improved. Furthermore, it becomes possible to dissociate double-stranded DNA into single-stranded DNA molecules by the action of temperature and subject the single-stranded DNA molecules to a measurement selectively. Furthermore, it also becomes possible to select the polarity of a DNA molecule and subject the DNA molecule to a measurement.

Abstract translation: 通过减缓纳米孔中DNA分子的通过速度，提高了DNA的核苷酸序列读数的准确性。 在具有不对称和周期性结构的DNA分子和携带DNA分子的纳米孔膜之间引入小的温差，由此通过纳米孔的DNA分子可以在一个方向上移动，并且DNA分子的通过速度在 纳米孔可以被控制和减少。 以这种方式，可以提高核苷酸序列分析的准确性。 此外，通过温度的作用将双链DNA解离成单链DNA分子成为可能，并将单链DNA分子选择性地进行测定。 此外，还可以选择DNA分子的极性并使DNA分子进行测量。

公开(公告)号：US20190329240A1

公开(公告)日：2019-10-31

申请号：US15998963

申请日：2016-02-17

Applicant: Hitachi High-Technologies Corporation

Inventor： Hirokazu KATO ,  Tatsuya YAMASHITA ,  Junji ISHIZUKA ,  Tomohiro SHOJI ,  Toshinari SAKURAI

IPC: B01L3/00 ,  G01N35/08 ,  G01N35/10 ,  B01L7/00

Abstract: A conventional solution exchanging method in a next-generation sequencer needs a reagent amount four or more times greater than an in-flow cell flow passage volume in order to efficiently promote a chemical reaction by replacing a reagent A in the flow cell with a new reagent B. Thus, the reagent consumption amount increases, and the cost is high. Meanwhile, an analysis apparatus according to the present invention is provided with: a flow cell used for analyzing samples; a sample container for containing a sample; a reagent container for containing a reagent; and a pressure generation mechanism for feeding the sample and the reagent to the flow cell through the flow passage, and also has an atmospheric opening in the flow passage of the flow cell on the upstream side.

公开(公告)号：US20170362634A1

公开(公告)日：2017-12-21

申请号：US15533087

申请日：2014-12-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yuichiro OTA ,  Tomohiro SHOJI ,  Toru YOKOYAMA ,  Masatoshi NARAHARA

IPC: C12Q1/68 ,  G01N21/05 ,  G01N21/64

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

公开(公告)号：US20160215330A1

公开(公告)日：2016-07-28

申请号：US15026150

申请日：2014-10-01

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Tsuyoshi SONEHARA ,  Tomohiro SHOJI

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/0636 ,  G01N2201/068

Abstract: A nucleic-acid-sequence determination device equipped with two light sources having different wavelengths, two detectors, and an optical system for irradiating a sample with light from the two light sources and guiding fluorescent light from a nucleic acid in the sample to the two detectors. The optical system is provided with a dichroic mirror for causing the fluorescent light from the nucleic acid in the sample to split, and guiding the split light to the two detectors. The dichroic mirror has a transition wavelength from transmission to reflection in two locations, namely: between light-emitting bands of two types of short-wavelength fluorescent dye and light-emitting bands of two types of long-wavelength fluorescent dye.

Abstract translation: 一种配备有具有不同波长的两个光源的核酸序列测定装置，两个检测器和用于从两个光源照射样品的光学系统并将来自样品中的核酸的荧光引导到两个检测器的光学系统 。 光学系统设置有用于使来自样品中的核酸的荧光分裂的分色镜，并将分离光引导到两个检测器。 二向色镜在两个位置具有从透射到反射的转变波长，即两种短波长荧光染料的发光带和两种长波长荧光染料的发光带之间。