-
公开(公告)号：US4184848A
公开(公告)日：1980-01-22
申请号：US836111
申请日：1977-09-23
IPC分类号： C07C69/28 , C12Q1/00 , G01N21/82 , G01N33/48 , G01N33/50 , G01N33/52 , G01N33/16 , G01N31/14
CPC分类号： C12Q1/00 , G01N33/50 , Y10T436/25125
摘要： An agent for the elimination of turbidity in serum, especially in serum samples wherein photometric measurements are to be made, consisting essentially of one or more mono- or di-esters of polyethylene glycol and fatty acids of 9 to 14 carbon atoms with at most 8 ethylene oxide units per fatty acid moiety in the molecule, said esters having an HLB value of from 8 to 12.8.
摘要翻译： 用于消除血清中浊度的试剂，特别是在其中进行光度测量的血清样品中，基本上由一种或多种聚乙二醇和9至14个碳原子的脂肪酸的单酯或二酯组成，最多8个 分子中每个脂肪酸部分的环氧乙烷单元，所述酯的HLB值为8至12.8。
-
公开(公告)号：US4337310A
公开(公告)日：1982-06-29
申请号：US184675
申请日：1980-09-08
CPC分类号： C08B31/003 , C12Q1/40
摘要： Method for determining .alpha.-amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of .alpha.-amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of .alpha.-amylase.
摘要翻译： 通过与淀粉底物孵育来测定α-淀粉酶的方法，其中所述底物是基于用可检测的基团衍生的用于测定α-淀粉酶的颗粒淀粉，所述底物由表面交联的淀粉或直链淀粉颗粒组成，所述淀粉或直链淀粉颗粒具有调节的， 所述颗粒具有0.01至0.20mm的直径; 这些颗粒通过将非溶胀颗粒悬浮在仅具有足够交联剂的溶液中以实现表面交联，然后用可检测的基团衍生化以测定α-淀粉酶来制备。
-
3.发明授权
公开(公告)号：US4436828A
公开(公告)日：1984-03-13
申请号：US200838
申请日：1980-10-27
IPC分类号： C07D249/20 , A61K31/22 , A61K31/265 , A61K31/275 , A61K31/40 , A61K31/44 , A61K31/445 , A61K31/70 , A61P43/00 , C07C43/32 , C07C67/00 , C07C69/34 , C07C69/42 , C07C69/67 , C07C205/43 , C07C253/00 , C07C255/16 , C07C327/22 , C07D207/40 , C07D207/46 , C07D209/48 , C07D213/63 , C07D227/12 , C07D249/18 , C07J19/00 , C07J41/00 , C07J43/00 , C07K19/00 , G01N33/53 , G01N33/531 , A61K43/00 , C07G7/00 , G01N33/56
CPC分类号： C07C255/00 , C07J41/0055 , C07J41/0094 , C07J43/003 , G01N33/531 , Y10S436/822 , Y10S436/823
摘要： New reactive asymmetrical dicarboxylic acid ester compounds of the formula ##STR1## wherein R.sub.1 is an alkyl radical containing up to 3 carbon atoms or is a digoxin or digitoxin residue, R.sub.2 and R.sub.3, which may be the same or different, are alkoxy radicals containing up to 3 carbon atoms or together represent an oxygen atom, X is a cyanomethoxy, succinimide-N-oxy, N-methyl-pyridiniumoxy, 2,4-dinitrophenoxy, 2,4,5-trichlorophenoxy, pentachlorophenoxy, phenylthio, p-nitrophenoxy, p-nitrophenylthio, piperidyl-N-oxy, phthalimido-N-oxy or benztriazol-N-oxy radical and n is 2, 3, 4 or 5, are useful for the preparation of reagents for the investigation of cardiac glycosides (Digitalis glycosides).
摘要翻译： 式（I）的新的反应性不对称二羧酸酯化合物，其中R 1是含有至多3个碳原子的烷基或是地高辛或二氧杂辛基残基，R2和R3可以相同或不同，是烷氧基 含有3个碳原子或一起代表氧原子的基团，X是氰基甲氧基，琥珀酰亚胺-N-氧基，N-甲基 - 吡啶氧基，2,4-二硝基苯氧基，2,4,5-三氯苯氧基，五氯苯氧基，苯硫基，p - 硝基苯氧基，对硝基苯硫基，哌啶基-N-氧基，苯二甲酰亚氨基-N-氧基或苯并三唑-N-氧基，n为2,3,4或5可用于制备用于研究强心苷的试剂（ 洋地黄糖苷）。
-
公开(公告)号：US4133949A
公开(公告)日：1979-01-09
申请号：US715020
申请日：1976-08-16
IPC分类号： C07D249/20 , A61K31/22 , A61K31/265 , A61K31/275 , A61K31/40 , A61K31/44 , A61K31/445 , A61K31/70 , A61P43/00 , C07C43/32 , C07C67/00 , C07C69/34 , C07C69/42 , C07C69/67 , C07C205/43 , C07C253/00 , C07C255/16 , C07C327/22 , C07D207/40 , C07D207/46 , C07D209/48 , C07D213/63 , C07D227/12 , C07D249/18 , C07J19/00 , C07J41/00 , C07J43/00 , C07K19/00 , G01N33/53 , G01N33/531
CPC分类号： C07C255/00 , C07J41/0055 , C07J41/0094 , C07J43/003 , G01N33/531 , Y10S436/822 , Y10S436/823
摘要： New reactive asymmetrical dicarboxylic acid ester compounds of the formula ##STR1## wherein R.sub.1 is an alkyl radical containing up to 3 carbon atoms or is a digoxin or digitoxin residue, R.sub.2 and R.sub.3, which may be the same or different, are alkoxy radicals containing up to 3 carbon atoms or together represent an oxygen atom, X is a cyanomethoxy, succinimide-N-oxy, N-methyl-pyridiniumoxy, 2,4-dinitrophenoxy, 2,4,5-trichlorophenoxy, pentachlorophenoxy, phenylthio, p-nitrophenoxy, p-nitrophenylthio, piperidyl-N-oxy, phthalimido-N-oxy or benztriazol-N-oxy radical and n is 2, 3, 4 or 5. are useful for the preparation of reagents for the investigation of cardiac glycosides (Digitalis glycosides).
摘要翻译： 式（I）的新的反应性不对称二羧酸酯化合物，其中R 1是含有至多3个碳原子的烷基或是地高辛或二氧杂辛基残基，R2和R3可以相同或不同，是烷氧基 含有最多3个碳原子或一起代表氧原子的基团，X是氰基甲氧基，琥珀酰亚胺-N-氧基，N-甲基 - 吡啶氧基，2,4-二硝基苯氧基，2,4,5-三氯苯氧基，五氯苯氧基，苯硫基，p - 硝基苯氧基，对硝基苯硫基，哌啶基-N-氧基，苯二甲酰亚氨基-N-氧基或苯并三唑-N-氧基，n为2,3,4或5.可用于制备用于研究强心苷的试剂（ 洋地黄糖苷）。
-
5.发明授权Alkylolamides of iodizable amino acids and immune test reagents containing them 失效可碘化氨基酸的烷基酰胺和含有它们的免疫试剂
公开(公告)号：US4202873A
公开(公告)日：1980-05-13
申请号：US782868
申请日：1977-03-30
IPC分类号： A61K31/165 , A61K31/415 , A61K51/00 , A61P43/00 , C07C20060101 , C07C67/00 , C07C231/00 , C07C231/02 , C07C231/12 , C07C237/20 , C07D233/54 , C07D233/64 , C07D233/90 , C07J19/00 , C07J41/00 , G01N33/60 , C07C103/78 , A61K43/00 , C07D233/02
CPC分类号： C07D233/64 , A61K31/165 , A61K31/415 , C07D233/54 , C07D233/90 , C07J41/0055 , G01N33/60
摘要： Novel alkylolamides of iodizable amino acids of the formula ##STR1## wherein, R.sup.1 is hydroxybenzyl, dihydroxybenzyl or imidazol-4-yl-methyl andR.sup.2 is monohydroxy-alkyl of 1 or 2 carbon atoms or dihydroxy-alkyl of up to 4 carbon atoms,and the water-soluble salts thereof are provided; these materials are useful as components of radioiodizable immune test reagents.
摘要翻译： 新颖的式可光化氨基酸的烷基酰胺，其中R1是羟基苄基，二羟基苄基或咪唑-4-基 - 甲基，R2是1或2个碳原子的单羟基烷基或至多4个碳原子的二羟基烷基， 并提供其水溶性盐; 这些材料可用作可放射性免疫测试试剂的组分。
-
公开(公告)号：US4268628A
公开(公告)日：1981-05-19
申请号：US22011
申请日：1979-03-19
CPC分类号： C08B31/00 , C12Q1/40 , C12Q2334/00 , Y10S435/805
摘要： Novel method for the determination of .alpha.-amylase which method comprises contacting a sample suspected of containing .alpha.-amylase with a starch derivative carrying a substituent capable of dyestuff-forming coupling; separating from the resulting starch-containing phase the low molecular weight soluble fission products formed by splitting of said starch derivative by .alpha.-amylase; coupling the said couplable substituent with another dyestuff forming component; and measuring the dyestuff so formed as a measure of the initial .alpha.-amylase content.
摘要翻译： 用于测定α-淀粉酶的新方法，该方法包括使怀疑含有α-淀粉酶的样品与携带能够进行染料形成偶联的取代基的淀粉衍生物接触; 从所得含淀粉相中分离出由α-淀粉酶分解所述淀粉衍生物形成的低分子量可溶裂解产物; 将所述可连接取代基与另一种形成染料的成分偶联; 并测量如此形成的染料作为初始α-淀粉酶含量的量度。
-
公开(公告)号：US4248786A
公开(公告)日：1981-02-03
申请号：US19017
申请日：1979-03-08
申请人： Hans-Georg Batz , Jurgen Horn , Klaus Stellner , Josef Maier , Michael Nelboeck-Hochstetter , Gunter Weimann
发明人： Hans-Georg Batz , Jurgen Horn , Klaus Stellner , Josef Maier , Michael Nelboeck-Hochstetter , Gunter Weimann
IPC分类号： C07D207/46 , C07K1/14 , C07K1/22 , C07K14/575 , C07K16/00 , C08F20/36 , C07G7/00
CPC分类号： C07D207/46 , C08F20/36
摘要： New hydroxy-succinimide ester compounds of the formula ##STR1## wherein X is selected from a carboxy-succinimide ester group and radicals of the general formulae: ##STR2## A is -O-CH.sub.2 -CH.sub.2 when n is 2 and X is a succinimide ester group but otherwise is a single bond;n is a whole number of from 2 to 7;m is 1, 2, 3 or 4,R is hydrogen, methyl, or cyanide; andR' is methyl or ethyl.have been found outstandingly useful as bridge-building compounds for linking proteins, especially biologically active proteins, to solid or liquid carrier matrix materials, thereby yielding valuable carrier-bound enzymatically active protein compositions.
摘要翻译： 新的式（I）的羟基 - 琥珀酰亚胺酯化合物，其中X选自羧基琥珀酰亚胺酯基和通式为CONHCH2CH（OR'）2 COOCH2CH2NHCON（R'）2的基团; 当n是2时，A是-O-CH 2 -CH 2，X是琥珀酰亚胺酯基，但是是单键; n是2到7的整数; m为1,2,3或4，R为氢，甲基或氰基; 和R'是甲基或乙基。 已经被发现作为用于将蛋白质，特别是生物活性蛋白质连接到固体或液体载体基质材料的桥接构建化合物非常有用，从而产生有价值的载体结合的酶活性蛋白质组合物。
-
公开(公告)号：US4155914A
公开(公告)日：1979-05-22
申请号：US815338
申请日：1977-07-13
申请人： Hans-Georg Batz , Jurgen Horn , Klaus Stellner , Josef Maier , Michael Nelboeck-Hochstetter , Gunter Weimann
发明人： Hans-Georg Batz , Jurgen Horn , Klaus Stellner , Josef Maier , Michael Nelboeck-Hochstetter , Gunter Weimann
IPC分类号： C07D207/46 , C07K1/14 , C07K1/22 , C07K14/575 , C07K16/00 , C08F20/36 , C07G7/00
CPC分类号： C07D207/46 , C08F20/36
摘要： New hydroxy-succinimide ester compounds of the formula ##STR1## wherein X is selected from a carboxy-succinimide ester group and radicals of the general formulae: ##STR2## A is --O--CH.sub.2 --CH.sub.2 when n is 2 and X is a succinimide ester group but otherwise is a single bond;N is a whole number of from 2 to 7;M is 1, 2, 3 or 4,R is hydrogen, methyl, or cyanide; andR' is methyl or ethyl;Have been found outstandingly useful as bridge-building compounds for linking proteins, especially biologically active proteins, to solid or liquid carrier matrix materials, thereby yielding valuable carrier-bound enzymatically active protein compositions.
摘要翻译： 新的式（I）的羟基 - 琥珀酰亚胺酯化合物，其中X选自羧基琥珀酰亚胺酯基和通式为：CONHCH2CH（OR'）2 COOCH2CH2NHCON（R'）2的基团; 当n是2时，A是-O-CH 2 -CH 2，X是琥珀酰亚胺酯基，但是是单键; N是2到7的全部数字; M为1,2,3或4，R为氢，甲基或氰基; 和R'是甲基或乙基; 已经被用作连接蛋白质，特别是生物活性蛋白质，固体或液体载体基质材料的桥梁建筑化合物，有效的载体有机化合物活性蛋白质组合物。
-
9.发明授权Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase 失效用于测定谷氨酸 - 乙酸肝素 - 转氨酶活性的方法和分析试剂
公开(公告)号：US4591553A
公开(公告)日：1986-05-27
申请号：US500111
申请日：1983-06-01
申请人： Ulfert Deneke , Wolfgang Gruber , Hans-Georg Batz
发明人： Ulfert Deneke , Wolfgang Gruber , Hans-Georg Batz
CPC分类号： C12Q1/52 , Y10S435/805
摘要： The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic acid, a chromogen, peroxidase, pyruvate oxidase, soluble phosphate, buffer and water, wherein the chromogen is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA) or 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole).
摘要翻译： 本发明提供了一种测定谷氨酸酰乙酰转氨酶（GOT）在水溶液中的活性的方法，其中（a）α-酮戊二酸与由GOT催化的丙氨酸 - 亚磺酸反应得到丙酮酸，二氧化硫和谷氨酸 ，（b）所得丙酮酸盐与磷酸盐和氧气反应，由丙酮酸氧化酶催化，得到乙酰磷酸盐，二氧化碳和过氧化氢，（c）过氧化氢和色原反应，由过氧化物酶催化，得到 染料和水，（d）确定形成的染料的量，其中使用的色原是N-（3'-磺酰基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA） 5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] - 咪唑（B-DDH-咪唑）或相关物质。 本发明还提供了用于测定GOT的活性的分析剂，其包括α-酮戊二酸，丙氨酸 - 亚磺酸，色原，过氧化物酶，丙酮酸氧化酶，可溶性磷酸盐，缓冲液和水，其中色原为N-（3 4-磺基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA）或4,5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] 咪唑（B-DDH-咪唑）。
-
公开(公告)号：US6117973A
公开(公告)日：2000-09-12
申请号：US805411
申请日：1997-02-24
申请人： Hans-Georg Batz , Henrik Frydenlund Hansen , Henrik Orum , Troels Koch , Gary B. Shuster , Bruce A. Armitage
发明人： Hans-Georg Batz , Henrik Frydenlund Hansen , Henrik Orum , Troels Koch , Gary B. Shuster , Bruce A. Armitage
CPC分类号： C07H21/00 , C07K14/003 , C12Q1/6818 , Y10S435/81
摘要： New electron transfer moiety labeled nucleic acid analogue probes are provided that can be used in methods for determining nucleic acids in a sample. The new probes can be prepared using novel monomer subunits in a chemical synthesis route. The nucleic acids can be determined by binding the probe molecules to the nucleic acid and inducing electron transfer within the complex formed. The occurrence of the electron transfer is determined as a measure of the nucleic acid.
摘要翻译： 提供了新的电子转移部分标记的核酸类似物探针，其可用于测定样品中核酸的方法。 可以在化学合成途径中使用新型单体亚单位制备新的探针。 核酸可以通过将探针分子与核酸结合并在形成的复合物内诱导电子转移来确定。 电子转移的发生被确定为核酸的量度。
-
-
-
-
-
-
-
-
-
搜索结果
46 条记录 (耗时 0.022 秒)