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29 条记录 (耗时 0.024 秒)

    Desensitized aspartokinase
    1.
    发明授权
    Desensitized aspartokinase 有权
    脱敏天冬氨酸激酶

    公开(公告)号：US06893848B1

    公开(公告)日：2005-05-17

    申请号：US09958606

    申请日：2000-04-14

    申请人： Haruhiko Yokoi ,  Junko Ohnishi ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Akio Ozaki

    发明人： Haruhiko Yokoi ,  Junko Ohnishi ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Akio Ozaki

    IPC分类号： C12N1/19 ,  C12N9/12 ,  C12P13/08 ,  C07C229/00 ,  C12N1/20 ,  C12N15/63 ,  C12N15/74 ,  C07N21/04

    CPC分类号： C12N9/1217 ,  C12P13/08

    摘要： The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

    摘要翻译： 本发明涉及一种衍生自棒状杆菌属细菌的新型天冬氨酸激酶; 编码酶的DNA; 含有上述DNA的重组DNA; 具有上述重组DNA的棒状细菌或其染色体上具有DNA的棒状细菌; 以及通过培养上述微生物来生产L-赖氨酸的方法。 已经成功制备了编码天冬氨酸的DNA，其不含由L-赖氨酸和由棒状杆菌衍生的L-苏氨酸的协同反馈抑制，并且具有编码氨基酸序列的核苷酸序列，其中311位的氨基酸残基是氨基酸 SEQ ID NO：18所示的氨基酸序列中的Thr除外。

    Glucose-6-phosphate dehydrogenase
    2.
    发明授权
    Glucose-6-phosphate dehydrogenase 有权
    葡萄糖-6-磷酸脱氢酶

    公开(公告)号：US07078204B2

    公开(公告)日：2006-07-18

    申请号：US10312007

    申请日：2001-06-15

    申请人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

    发明人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

    IPC分类号： C12N9/04 ,  C12N1/20 ,  C12P21/04 ,  C12P13/04 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/08 ,  C12Y101/01049

    摘要： The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

    摘要翻译： 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶（以下称为“G6PD”），编码该酶的DNA，包含该DNA的重组DNA，包含重组体 DNA，包含其染色体上的DNA的转化体，以及生产L-氨基酸或G6PD的方法，其包括培养转化体。 根据本发明，获得了修饰的G6PD和编码G6PD的D​​NA，通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。

    Process for producing HMG-CoA reductase inhibitors
    5.
    发明授权
    Process for producing HMG-CoA reductase inhibitors 失效
    生产HMG-CoA还原酶抑制剂的方法

    公开(公告)号：US06946270B1

    公开(公告)日：2005-09-20

    申请号：US09868924

    申请日：2000-01-20

    申请人： Shin-ichi Hashimoto ,  Yoshiyuki Yonetani ,  Akio Ozaki

    发明人： Shin-ichi Hashimoto ,  Yoshiyuki Yonetani ,  Akio Ozaki

    IPC分类号： C12P7/62 ,  C12P17/06

    CPC分类号： C12P17/06 ,  C12P7/62

    摘要： The present invention relates to a process for producing a compound (II-a) or a compound (II-b), each of which is a hydroxylated product of a compound represented by the formula (I-a) (hereinafter referred to as compound (I-a)): wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and R2 represents a substituted or unsubstituted alkyl, or a substituted or unsubstituted aryl; or a ring-closed lactone form thereof (hereinafter referred to as compound (I-b)). wherein the process comprises: treating the compound (I-a) or compound (I-b) in an aqueous medium comprising with a microorganism acting to hydroxylate compound (I-a) or compound (I-b), having no ability to sporulate and showing no hyphal growth, a culture of the microorganism, or a treated product of the culture, as an enzyme source; and collecting a hydroxylated product of compound (I-a) or compound (I-b) from the aqueous medium.

    摘要翻译： 本发明涉及一种制备化合物（II-a）或化合物（II-b）的方法，其中每种化合物是由式（Ia）表示的化合物的羟基化产物（以下称为化合物（Ia ））：其中R 1表示氢原子，取代或未取代的烷基或碱金属，R 2表示取代或未取代的烷基，或取代或未取代的烷基， 未取代的芳基; 或其封闭的内酯形式（以下称为化合物（I-b））。 其中所述方法包括：在水性介质中处理化合物（Ia）或化合物（Ib），所述水性介质包含微生物作用于羟化化合物（Ia）或化合物（Ib）的微生物，其不具有孢子形成和不显示菌丝生长的能力， 的微生物或培养物的处理产物作为酶源; 并从水性介质中收集化合物（I-a）或化合物（I-b）的羟基化产物。

    Protein and DNA encoding the protein
    10.
    发明授权
    Protein and DNA encoding the protein 失效
    编码蛋白质的蛋白质和DNA

    公开(公告)号：US08058037B2

    公开(公告)日：2011-11-15

    申请号：US12095244

    申请日：2006-11-29

    申请人： Shin-ichi Hashimoto ,  Yoshiyuki Yonetani ,  Masaki Maeda

    发明人： Shin-ichi Hashimoto ,  Yoshiyuki Yonetani ,  Masaki Maeda

    IPC分类号： C12P7/00 ,  C12P7/02

    CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12P7/42 ,  C12P17/06

    摘要： The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).

    摘要翻译： 本发明提供了包含SEQ ID NO：1〜3中任一项所示的氨基酸序列的蛋白质（除了SEQ ID NO：4〜6所示的氨基酸序列的蛋白质）和工业上的工序 有利地产生抑制HMG-CoA还原酶并具有降低血清胆固醇的作用的化合物，使用编码包含SEQ ID NO：1至3中任一项所示的氨基酸序列的蛋白的DNA（除了编码包含 如SEQ ID NO：4所示的氨基酸序列）。