公开(公告)号：US06893848B1

公开(公告)日：2005-05-17

申请号：US09958606

申请日：2000-04-14

申请人： Haruhiko Yokoi ,  Junko Ohnishi ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Akio Ozaki

发明人： Haruhiko Yokoi ,  Junko Ohnishi ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Akio Ozaki

IPC分类号： C12N1/19 ,  C12N9/12 ,  C12P13/08 ,  C07C229/00 ,  C12N1/20 ,  C12N15/63 ,  C12N15/74 ,  C07N21/04

CPC分类号： C12N9/1217 ,  C12P13/08

摘要： The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

摘要翻译： 本发明涉及一种衍生自棒状杆菌属细菌的新型天冬氨酸激酶; 编码酶的DNA; 含有上述DNA的重组DNA; 具有上述重组DNA的棒状细菌或其染色体上具有DNA的棒状细菌; 以及通过培养上述微生物来生产L-赖氨酸的方法。 已经成功制备了编码天冬氨酸的DNA，其不含由L-赖氨酸和由棒状杆菌衍生的L-苏氨酸的协同反馈抑制，并且具有编码氨基酸序列的核苷酸序列，其中311位的氨基酸残基是氨基酸 SEQ ID NO：18所示的氨基酸序列中的Thr除外。

公开(公告)号：US07078204B2

公开(公告)日：2006-07-18

申请号：US10312007

申请日：2001-06-15

申请人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

发明人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12P21/04 ,  C12P13/04 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/08 ,  C12Y101/01049

摘要： The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

摘要翻译： 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶（以下称为“G6PD”），编码该酶的DNA，包含该DNA的重组DNA，包含重组体 DNA，包含其染色体上的DNA的转化体，以及生产L-氨基酸或G6PD的方法，其包括培养转化体。 根据本发明，获得了修饰的G6PD和编码G6PD的D​​NA，通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。

公开(公告)号：US07332310B2

公开(公告)日：2008-02-19

申请号：US10805394

申请日：2004-03-22

申请人： Satoshi Nakagawa ,  Hiroshi Mizoguchi ,  Seiko Ando ,  Mikiro Hayashi ,  Keiko Ochiai ,  Haruhiko Yokoi ,  Naoko Tateishi ,  Akihiro Senoh ,  Masato Ikeda ,  Akio Ozaki

发明人： Satoshi Nakagawa ,  Hiroshi Mizoguchi ,  Seiko Ando ,  Mikiro Hayashi ,  Keiko Ochiai ,  Haruhiko Yokoi ,  Naoko Tateishi ,  Akihiro Senoh ,  Masato Ikeda ,  Akio Ozaki

IPC分类号： C12P13/08 ,  C12N1/21 ,  C07H21/04 ,  C12N9/04

CPC分类号： C12N9/93 ,  C07K14/34 ,  C12N9/0004 ,  C12P13/08 ,  C12R1/15 ,  C12Y101/01003 ,  C12Y604/01001

摘要： Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

摘要翻译： 衍生自属于棒状细菌的微生物及其片段的新型多核苷酸，由多核苷酸及其片段编码的多肽，包含多核苷酸及其片段的多核苷酸阵列，其中已记录了多核苷酸及其片段的核苷酸序列是可读的 在电脑中，并使用它们。

公开(公告)号：US20060228712A1

公开(公告)日：2006-10-12

申请号：US10805394

申请日：2004-03-22

申请人： Satoshi Nakagawa ,  Hiroshi Mizoguchi ,  Seiko Ando ,  Mikiro Hayashi ,  Keiko Ochiai ,  Haruhiko Yokoi ,  Naoko Tateishi ,  Akihiro Senoh ,  Masato Ikeda ,  Akio Ozaki

发明人： Satoshi Nakagawa ,  Hiroshi Mizoguchi ,  Seiko Ando ,  Mikiro Hayashi ,  Keiko Ochiai ,  Haruhiko Yokoi ,  Naoko Tateishi ,  Akihiro Senoh ,  Masato Ikeda ,  Akio Ozaki

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12P19/34 ,  C12N9/00 ,  C12M1/34 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/93 ,  C07K14/34 ,  C12N9/0004 ,  C12P13/08 ,  C12R1/15 ,  C12Y101/01003 ,  C12Y604/01001

摘要： Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

摘要翻译： 衍生自属于棒状细菌的微生物及其片段的新型多核苷酸，由多核苷酸及其片段编码的多肽，包含多核苷酸及其片段的多核苷酸阵列，其中记录了多核苷酸及其片段的核苷酸序列是可读的记录介质 在电脑中，并使用它们。

公开(公告)号：US20100120088A1

公开(公告)日：2010-05-13

申请号：US12430592

申请日：2009-04-27

申请人： Masato IKEDA ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

发明人： Masato IKEDA ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

IPC分类号： C12P21/00 ,  C12P13/04 ,  C12P19/30 ,  C12P19/00 ,  C12P13/06 ,  C12P13/08 ,  C12P13/22

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

公开(公告)号：US20050079586A1

公开(公告)日：2005-04-14

申请号：US10497502

申请日：2002-12-03

申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

IPC分类号： C12N1/21 ,  C12N9/04 ,  C12N15/53 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/22 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码该多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

公开(公告)号：US07524667B2

公开(公告)日：2009-04-28

申请号：US10497502

申请日：2002-12-03

申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

公开(公告)号：US07989191B2

公开(公告)日：2011-08-02

申请号：US12430592

申请日：2009-04-27

申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12P13/04 ,  C12P21/04 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

公开(公告)号：US06468780B2

公开(公告)日：2002-10-22

申请号：US09829080

申请日：2001-04-10

申请人： Yutaka Takano ,  Masaru Hasegawa ,  Hideo Mori ,  Katsuhiko Ando ,  Keiko Ochiai ,  Hiroaki Motoyama ,  Akio Ozaki

发明人： Yutaka Takano ,  Masaru Hasegawa ,  Hideo Mori ,  Katsuhiko Ando ,  Keiko Ochiai ,  Hiroaki Motoyama ,  Akio Ozaki

IPC分类号： C12N120

CPC分类号： C12R1/07 ,  C12P7/62 ,  C12P17/06

摘要： Disclosed is a process for producing a compound represented by general formula (II-a): (wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl group, or an alkali metal; and R2 represents a substituted or unsubstituted alkyl or aryl group) [hereinafter referred to as Compound (II-a)] or the lactone form of Compound (II-a) [hereinafter referred to as Compound (II-b)] which comprises subjecting a compound represented by general formula (I-a): (wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl group, or an alkali metal; and R2 represents a substituted or unsubstituted alkyl or aryl group) [hereinafter referred to as Compound (I-a)] or the lactone form of Compound (I-a) [hereinafter referred to as Compound (I-b)] to the action of an enzyme source derived from a microorganism belonging to the genus Bacillus and capable of converting Compound (I-a) or Compound (I-b) into Compound (II-a) or Compound (II-b) in a reaction mixture to form Compound (II-a) or Compound (II-b) in the reaction mixture, and recovering Compound (II-a) or Compound (II-b) from the reaction mixture.

摘要翻译： 公开了一种制备由通式（II-a）表示的化合物的方法：其中R 1表示氢原子，取代或未取代的烷基或碱金属; R 2表示取代或未取代的烷基或芳基） [化学式（II-a）]或化合物（II-a）的内酯形式[以下称为化合物（II-b）]] [其中包括使通式（Ia）表示的化合物 R1表示氢原子，取代或未取代的烷基或碱金属; R2表示取代或未取代的烷基或芳基）[以下称为化合物（Ia）]或化合物（Ia）的内酯形式[ （Ia）或化合物（Ib）转化为化合物（II-a）或化合物（II-a）的酶源的作用，以下称为化合物（Ib） b）在反应混合物中形成化合物（II -a）或化合物（II-b），并从反应混合物中回收化合物（II-a）或化合物（II-b）。

公开(公告)号：US06413748B1

公开(公告)日：2002-07-02

申请号：US09455406

申请日：1999-12-06

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12P1324

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用转化体产生L-脯氨酸-3-羟化酶的方法。