摘要:
Provided are SARS-CoV detection PCR primers having nucleotide sequences as set forth in SEQ ID NOS: 1-46, a method for detecting SARS-CoV using the primers, and a SARS-CoV detection kit including the primers.
摘要翻译:提供具有SEQ ID NO:1-46所示核苷酸序列的SARS-CoV检测PCR引物,使用引物检测SARS-CoV的方法和包含引物的SARS-CoV检测试剂盒。
摘要:
Provided are SARS-CoV detection PCR primers having nucleotide sequences as set forth in SEQ ID NOS: 1-46, a method for detecting SARS-CoV using the primers, and a SARS-CoV detection kit including the primers.
摘要翻译:提供具有SEQ ID NO:1-46所示核苷酸序列的SARS-CoV检测PCR引物,使用引物检测SARS-CoV的方法和包含引物的SARS-CoV检测试剂盒。
摘要:
Provided are a primer set selected from the group consisting of primers having nucleotide sequences as set forth in SEQ ID NOS: 1–40, a method for detecting hepatitis B virus by polymerase chain reaction (PCR) using the primer set, and a hepatitis B virus detection kit including the primer set.
摘要翻译:提供了选自由SEQ ID NO:1-40所示的核苷酸序列的引物组成的引物组,使用引物组的聚合酶链反应(PCR)检测乙型肝炎病毒的方法,以及乙型肝炎 病毒检测试剂盒包括引物组。
摘要:
Provided are a primer set selected from the group consisting of primers having nucleotide sequences as set forth in SEQ ID NOS: 1-40, a method for detecting hepatitis B virus by polymerase chain reaction (PCR) using the primer set, and a hepatitis B virus detection kit including the primer set.
摘要翻译:提供了选自由SEQ ID NO:1-40所示的核苷酸序列的引物组成的引物组,使用引物组的聚合酶链反应(PCR)检测乙型肝炎病毒的方法,以及乙型肝炎 病毒检测试剂盒包括引物组。
摘要:
A method for real-time detection of polymerase chain reaction (PCR) is provided. The method includes: manufacturing a reactor having a plurality of electrodes; immobilizing a PCR primer to surfaces of electrodes; injecting a mixture for a PCR into the reactor to perform the PCR on the surfaces of electrodes and measuring an impedance of the PCR product.
摘要:
A method for real-time detection of polymerase chain reaction (PCR) is provided. The method includes: manufacturing a reactor having a plurality of electrodes; immobilizing a PCR primer to surfaces of electrodes; injecting a mixture for a PCR into the reactor to perform the PCR on the surfaces of electrodes and measuring an impedance of the PCR product.
摘要:
A microfluidic device which electrochemically regulates a pH of a fluid, and a method of regulating a pH of a fluid using the microfluidic device, include a chamber which includes a cathode formed of a metal adsorbing hydrogen gas, and an anode formed of a metal having a higher standard oxidation potential than, and does not react with, water.
摘要:
Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要:
Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要:
A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber