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    Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group
    6.
    发明申请
    Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group 审中-公开
    使用涂覆有羧基或氨基的固相材料来扩增核酸的方法

    公开(公告)号：US20050186602A1

    公开(公告)日：2005-08-25

    申请号：US11042376

    申请日：2005-01-25

    申请人： Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

    发明人： Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

    IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1006

    摘要： A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.

    摘要翻译： 提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。 该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物 用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。

    Method of concentrating and disrupting cells or viruses
    7.
    发明申请
    Method of concentrating and disrupting cells or viruses 审中-公开
    浓缩和破坏细胞或病毒的方法

    公开(公告)号：US20080090275A1

    公开(公告)日：2008-04-17

    申请号：US11783351

    申请日：2007-04-09

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    IPC分类号： C12N13/00

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12N13/00 ,  G01N33/54326

    摘要： Provided is a method for concentrating and disrupting cells or viruses, the method employs magnetic particles which are capable of binding the cells or viruses to capture the cells or viruses and a laser to disrupt the cells or viruses. Since the same particles are used in target cell separation and cell lysis, there is no need to add additional particles in a laser lysis process, and thus integration of a target cell separation, concentration, purification, and nucleic acid extraction process is easy.

    摘要翻译： 提供了一种用于浓缩和破坏细胞或病毒的方法，该方法使用能够结合细胞或病毒以捕获细胞或病毒的磁性颗粒和激光以破坏细胞或病毒。 由于在靶细胞分离和细胞裂解中使用相同的颗粒，因此不需要在激光裂解过程中添加额外的颗粒，因此靶细胞分离，浓缩，纯化和核酸提取过程的整合是容易的。

    Apparatus for circulating carrier fluid
    8.
    发明授权
    Apparatus for circulating carrier fluid 失效
    载体流体循环装置

    公开(公告)号：US07329535B2

    公开(公告)日：2008-02-12

    申请号：US10292012

    申请日：2002-11-11

    申请人： Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

    发明人： Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

    IPC分类号： C12M1/34 ,  C12M3/00 ,  F16K13/00 ,  B01L11/00 ,  B32B5/02

    CPC分类号： B01L7/525 ,  B01L3/502738 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  Y10T137/4643 ,  Y10T137/4651

    摘要： Provided are an apparatus for circulating a carrier fluid having two or more chambers or sections, an apparatus for amplifying a nucleic acid using the same, and a chip containing the same. The apparatus for circulating a carrier fluid includes two or more chambers maintained at different temperatures, each chamber having an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port), and an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port), wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.

    摘要翻译： 提供一种用于循环具有两个或更多个室或部分的载体流体的装置，用于使用其的核酸的放大装置和含有该核酸的芯片的装置。 用于循环载体流体的装置包括保持在不同温度的两个或更多个室，每个室具有入口阀，该入口阀包含用于控制载体流体流入室（进气气压端口）的气动空气压力端口，以及出口 阀包含用于控制从腔室（出口气动空气压力端口）流出的载气的气动空气压力，其中腔室顺序地连接，使得一个室的出口阀连接到相邻室的入口阀 方向流体流动。

    Centrifugal force based microfluidic device for dilution and microfluidic system including the same
    9.
    发明授权
    Centrifugal force based microfluidic device for dilution and microfluidic system including the same 失效
    用于稀释和微流体系统的基于离心力的微流体装置包括相同的

    公开(公告)号：US07951332B2

    公开(公告)日：2011-05-31

    申请号：US12029480

    申请日：2008-02-12

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    IPC分类号： B01L11/00

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677

    摘要： Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

    摘要翻译： 提供了一种能够自动执行稀释操作的离心力微流体装置和包括其的微流体系统。 用于稀释的基于离心力的微流体装置包括可旋转盘式平台，设置在平台上的混合室; 缓冲溶液储存器，其设置在所述平台的比混合室更靠近所述平台的中心的部分上，所述缓冲溶液存储器经由通道至少一次向所述混合室提供预定量的缓冲溶液，所述通道连接到所述混合室， 并且多个稀释溶液室通过从对应于预定水的中间出口延伸的流路连接到平台的比混合室更远离平台中心的部分上的稀释溶液室 至少一次连续地接收在混合室中连续稀释的溶液。

    Cell lysis by heating-cooling process through endothermic reaction
    10.
    发明授权
    Cell lysis by heating-cooling process through endothermic reaction 有权
    通过吸热反应通过加热 - 冷却过程进行细胞裂解

    公开(公告)号：US07521246B2

    公开(公告)日：2009-04-21

    申请号：US11335414

    申请日：2006-01-19

    申请人： Ji-yeon Yang ,  Yoon-kyoung Cho ,  Jin-tae Kim ,  Sook-young Kim ,  Young-sun Lee

    发明人： Ji-yeon Yang ,  Yoon-kyoung Cho ,  Jin-tae Kim ,  Sook-young Kim ,  Young-sun Lee

    IPC分类号： G01N33/48

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12M47/20 ,  Y10T436/25

    摘要： Provided is a cell lysis method including: preparing a cell sample to be lysed; heating the cell sample; and cooling the cell sample by causing an endothermic reaction near the cell sample. According to the method, cell lysis can be simply and conveniently performed without regard to location and without additional devices since a separate energy source is not required and the apparatus is portable. In particular, when cell lysis is performed in a biochip using a small amount of sample, a greater cell lysis effect can be obtained. In addition, cell lysis efficiency is significantly improved, compared to when only heating is performed.

    摘要翻译： 提供了一种细胞裂解方法，包括：制备待裂解的细胞样品; 加热细胞样品; 并通过在细胞样品附近引起吸热反应来冷却细胞样品。 根据该方法，可以简单且方便地进行细胞裂解，而不考虑位置和不需要额外的装置，因为不需要单独的能源并且该装置是便携式的。 特别是，在使用少量样品的生物芯片中进行细胞裂解时，可以获得更大的细胞裂解效果。 此外，与仅进行加热时相比，细胞裂解效率显着提高。