公开(公告)号：US4971677A

公开(公告)日：1990-11-20

申请号：US310645

申请日：1989-02-15

申请人： Hideki Kambara ,  Yoshiko Katayama ,  Tetsuo Nishikawa

发明人： Hideki Kambara ,  Yoshiko Katayama ,  Tetsuo Nishikawa

IPC分类号： G01N33/50 ,  G01N27/447

CPC分类号： G01N27/44721

摘要： In an electrophoresis apparatus for separating and detecting a sample of DNA or RNA labelled with fluorescence, use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in order to greatly shorten the time required for the measurement.

公开(公告)号：US5062942A

公开(公告)日：1991-11-05

申请号：US506986

申请日：1990-04-10

申请人： Hideki Kambara ,  Tetsuo Nishikawa ,  Tomoaki Sumitani ,  Keiichi Nagai ,  Yoshitoshi Ito

发明人： Hideki Kambara ,  Tetsuo Nishikawa ,  Tomoaki Sumitani ,  Keiichi Nagai ,  Yoshitoshi Ito

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N21/6456 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6469

摘要： In a fluorescence detection type electrophoresis apparatus, comprising an electrophoresis separation gel plate (2, 102, 205), an excitation laser beam source (3, 201, 201) for emitting fluorescent light, and a fluorescent light detector (9, 109, 212) for detecting the emitted fluorescent light, a fluorescent light images are divided into a plurality of virtual images by an image divider (5, 7.sub.2, 104, 105, 209) and at the same time, the lights corresponding to the individual divided images are wavelength-selected by bandpass filters (8, 8.sub.3, 107, 108, 120, 227-230), thereby providing highly accurate, sensitive separation and detection of DNA fragments and the like and determination of base sequence of DNA and the like.

摘要翻译： 在荧光检测型电泳装置中，包括电泳分离凝胶板​​（2,102,205），用于发射荧光的激发激光束源（3,201,201）和荧光检测器（9,109,212 ），用于检测发射的荧光，荧光灯图像由图像分割器（5,72,104,105,209）分割成多个虚拟图像，同时，对应于各个分割图像的光是 通过带通滤波器（8,83,107,108,120,227-230）波长选择，从而提供DNA片段等的高度精确，灵敏的分离和检测以及DNA的碱基序列的测定等。

公开(公告)号：US4879012A

公开(公告)日：1989-11-07

申请号：US211284

申请日：1988-06-24

申请人： Hideki Kambara ,  Tetsuo Nishikawa

发明人： Hideki Kambara ,  Tetsuo Nishikawa

IPC分类号： G01N27/26 ,  B01D57/02 ,  G01N27/447

CPC分类号： G01N27/44704 ,  B01D57/02

摘要： An electrophoresis gel is regenerated by light-irradiating an electrophoresis gel used for separating a fluorescence-labeled sample by electrophoresis and optically detecting the sample, thereby photo-dissociating a fluorescence-labeling substance remaining in the electrophoresis gel and reutilizing the resulting gel repeatedly. A troublesome operation of preparing an electrophoresis gel at every occasion of separation and detection can be saved thereby.

摘要翻译： 通过电泳对用于分离荧光标记样品的电泳凝胶进行光照射，并对样品进行光学检测，从而光电离残留在电泳凝胶中的荧光标记物质并反复再利用，从而再生电泳凝胶。 因此可以节省在分离和检测的每个场合制备电泳凝胶的麻烦的操作。

公开(公告)号：US5307148A

公开(公告)日：1994-04-26

申请号：US684113

申请日：1991-04-12

申请人： Hideki Kambara ,  Keiichi Nagai ,  Tamotu Simada ,  Tetsuo Nishikawa ,  Tomoaki Sumitani

发明人： Hideki Kambara ,  Keiichi Nagai ,  Tamotu Simada ,  Tetsuo Nishikawa ,  Tomoaki Sumitani

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N21/645 ,  G01N27/44721 ,  G01N2021/6441

摘要： A multicolor fluorescence detection type electrophoresis apparatus comprises an electrophoretic device having migration lanes in which fragment groups specifically labeled by fluorophores of different light emission peak wavelengths are mixed, added, and migrated. It identifies and detects the fragment groups. It uses laser beams of different wavelengths for exciting the fluorophores in the migration lanes. The laser beams are irradiated to separate positions on the migration lanes for detection of a few kinds of fluorophores at irradiation positions. For the purpose, multicolor optical filters are arranged near the irradiation positions to identify and detect lights emitted from the fluorophores excited by wavelength at every irradiation position.

摘要翻译： 多色荧光检测型电泳装置包括具有迁移通道的电泳装置，其中由不同发光峰波长的荧光团特异性标记的片段组被混合，添加和迁移。 它识别和检测片段组。 它使用不同波长的激光束激发迁移通道中的荧光团。 将激光束照射到迁移道上的分离位置，以便在照射位置检测几种荧光团。 为此，在照射位置附近设置多色滤光片，以识别和检测在每个照射位置由被波长激发的荧光团发出的光。

公开(公告)号：US5356776A

公开(公告)日：1994-10-18

申请号：US942470

申请日：1992-09-09

申请人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

发明人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

IPC分类号： C12M1/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/70

CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N27/447 ,  Y10T436/143333

摘要： DNA molecule length can be measured with high precision and efficiency by 1) using such means as electrophoresis gel migration to orient a DNA molecule having a fluorescence label at both its termini into a straight line by its passing through a migration path having in a portion of it an area not more than several micrometers in diameter, detecting the fluorescence label at both the termini at a predetermined location and measuring the interval between the detection of the fluorescence coming from one terminus and that of the fluorescence from the other or by 2) a DNA molecule bound to a fluorescence label at one terminus and to a particle at the other being led as a whole by such means as electric field application into an aperture smaller in diameter than the particle, leaving the particle fixed at the mouth of the aperture to stretch the DNA molecule and detecting the fluorescence position to measure the distance between the bound particle and the bound fluorescence label.

摘要翻译： 可以通过以下方式测量DNA分子长度：1）使用电泳凝胶迁移的方式，通过其经过一部分迁移路径将具有两端的荧光标记的DNA分子定向成直线 它是直径不超过几微米的区域，在预定位置的两个末端检测荧光标记，并测量从一个末端检测荧光和来自另一个末端的荧光检测之间的间隔，或者通过2）a 在一个末端与一个荧光标记结合的DNA分子和另一个的一个粒子整体通过电场施加到比颗粒直径更小的孔隙中，将颗粒固定在孔的口处 拉伸DNA分子并检测荧光位置以测量结合的颗粒和结合的荧光标记之间的距离。

公开(公告)号：US20060193497A1

公开(公告)日：2006-08-31

申请号：US11349962

申请日：2006-02-09

申请人： Ichiro Matsumoto ,  Tetsuo Nishikawa

发明人： Ichiro Matsumoto ,  Tetsuo Nishikawa

IPC分类号： G06K9/00

CPC分类号： G06T7/0004 ,  G06T7/12 ,  G06T2207/30148

摘要： (A) Luminance data is prepared. (B) A group of first order differential values is obtained from luminance values of said luminance data along a first direction, a group of second order differential values is obtained from the group of first order differential values, and first data is output in accordance with the group of second order differential values. There is provided a stain inspection method having high inspection correctness.

摘要翻译： （A）准备亮度数据。 （B）从第一方向的所述亮度数据的亮度值获得一组一阶微分值，从一阶微分值组获得一组二阶微分值，并且根据 一组二阶微分值。 提供了具有高检查正确性的污渍检查方法。

公开(公告)号：US07010141B2

公开(公告)日：2006-03-07

申请号：US10717642

申请日：2003-11-21

申请人： Hideaki Sugiura ,  Taizo Nakata ,  Tetsuo Nishikawa

发明人： Hideaki Sugiura ,  Taizo Nakata ,  Tetsuo Nishikawa

IPC分类号： H04R25/00

CPC分类号： H04R9/043 ,  H04R31/006

摘要： In a speaker device 10 having: a magnetic circuit 13 including a magnet 14 and a yoke 11; a voice coil 16 which is placed in a magnetic gap 12 of the magnetic circuit 13; and a diaphragm 17 which is vibrating available in a state where the diaphragm is coupled with the voice coil 16, a peripheral edge of the diaphragm 17 being fixed to a housing 20, a damper holder 30 is disposed in a portion where a damper 27 is attached to the housing 20. The damper holder 30 is in point contact with the housing 20 via three or more projections 31 which are disposed on one of the damper holder 30 and the housing 20. Preferably, the damper holder 30 is made of a hybrid material of a resin (polypropylene) and tungsten, and has a specific gravity of 10.0 or larger.

摘要翻译： 在具有：具有磁铁14和磁轭11的磁路13的扬声器装置10中， 放置在磁路13的磁隙12中的音圈16; 以及在隔膜与音圈16连接的状态下可振动的隔膜17，隔膜17的周缘固定在壳体20上，阻尼器保持件30设置在阻尼器27为止的部分 阻尼器保持器30经由设置在阻尼器保持器30和壳体20中的一个上的三个或更多个突起31与壳体20点接触。优选地，阻尼器保持器30由混合物 树脂（聚丙烯）和钨的材料，比重为10.0以上。

公开(公告)号：US20050250144A1

公开(公告)日：2005-11-10

申请号：US11109156

申请日：2005-04-19

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/12 ,  C12N9/16 ,  C12N15/09 ,  C12P21/06

CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C12N2799/021

摘要： Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Two novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.

摘要翻译： 已经在螺旋研究所（螺旋克隆，日本专利申请2000-183767）中确定了已经分离的克隆的克隆和/或磷酸酶样结构的克隆及其结构的选择。 通过使用已知的激酶和磷酸酶的氨基酸序列作为查询，通过对所有螺旋克隆进行同源性检索来提供两个新基因。 这些基因预期参与细胞内信号转导。 可以通过使用能够检测信号转导的报告基因测定系统来测试本发明基因的生理功能。 本发明的蛋白质可用作药物发现和开发新药物中的靶分子。

公开(公告)号：US5221852A

公开(公告)日：1993-06-22

申请号：US829189

申请日：1992-02-03

申请人： Eiichi Nagai ,  Tetsuo Nishikawa

发明人： Eiichi Nagai ,  Tetsuo Nishikawa

IPC分类号： H01L29/762 ,  H01L21/339 ,  H01L27/148 ,  H01L29/768 ,  H04N5/335 ,  H04N5/341 ,  H04N5/357 ,  H04N5/372

CPC分类号： H01L29/76833 ,  H01L27/14831

摘要： A charge coupled device (CCD) has a charge storage region and a potential barrier region. The CCD includes a first layer made of a first conductivity type semiconductor, a second layer made of a second conductivity type semiconductor and provided on the first layer, where the first and second conductivity types are mutually opposite types selected from n-type and p-type semiconductors, a third layer made of a first conductivity type semiconductor, impurity diffusion regions provided in at least a surface part of the third layer and having an impurity density higher than that of the third layer, a first gate electrode provided on the third layer between two mutually adjacent impurity diffusion regions, and a second gate electrode provided on each impurity diffusion region of the third layer. The impurity diffusion region forms the charge storage region of the CCD and the third layer between the two mutually adjacent impurity diffusion regions forms the potential barrier region of the CCD.

摘要翻译： 电荷耦合器件（CCD）具有电荷存储区域和势垒区域。 CCD包括由第一导电类型半导体制成的第一层，由第二导电类型半导体制成的第二层，设置在第一层上，其中第一和第二导电类型是相互相反的类型，选自n型和p-型， 型半导体，由第一导电型半导体制成的第三层，在第三层的至少表面部分设置的杂质浓度高于第三层的杂质浓度的杂质扩散区，设置在第三层上的第一栅电极 在两个相互相邻的杂质扩散区之间，以及设置在第三层的每个杂质扩散区上的第二栅电极。 杂质扩散区域形成CCD的电荷存储区域，并且两个相互相邻的杂质扩散区域之间的第三层形成CCD的势垒区域。

公开(公告)号：US20080069826A1

公开(公告)日：2008-03-20

申请号：US11869267

申请日：2007-10-09

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

IPC分类号： A61K39/00 ,  A61P43/00 ,  C07K16/18

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C07K14/4702 ,  C12N2799/021

摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.

摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。