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20 条记录 (耗时 0.019 秒)

    Fixation of nucleotide derivatives to solid carrier
    1.
    发明授权
    Fixation of nucleotide derivatives to solid carrier 有权
    核苷酸衍生物固定于固体载体

    公开(公告)号：US06664051B1

    公开(公告)日：2003-12-16

    申请号：US09706405

    申请日：2000-11-03

    申请人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    IPC分类号： C07H2104

    CPC分类号： C40B40/06 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00677 ,  B82Y30/00 ,  C40B50/06

    摘要： A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

    摘要翻译： 通过以下方法制备DNA芯片或其由连接于固体载体上的核苷酸衍生物组成的类似物的方法：使得在每个末端具有反应性基团的核苷酸衍生物与其中具有反应性基团的固体载体在水相中存在下接触 能够通过核苷酸衍生物的反应性基团和固体载体的反应性基团的重排而产生共价键的转移酶。

    Fluorescent substances
    4.
    发明授权
    Fluorescent substances 失效
    荧光物质

    公开(公告)号：US06642375B2

    公开(公告)日：2003-11-04

    申请号：US09731279

    申请日：2000-12-06

    申请人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    发明人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    IPC分类号： C07H1904

    CPC分类号： G01N33/582

    摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.

    摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。

    Homogenous enzyme immunoassay process
    5.
    发明授权
    Homogenous enzyme immunoassay process 失效
    均质酶免疫测定法

    公开(公告)号：US06287785B1

    公开(公告)日：2001-09-11

    申请号：US09488371

    申请日：2000-01-20

    申请人： Hiroshi Shinoki ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Osamu Seshimoto

    IPC分类号： G01N33533

    CPC分类号： G01N33/535 ,  G01N33/542 ,  G01N33/54306 ,  Y10S435/963 ,  Y10S435/964 ,  Y10S435/969 ,  Y10S435/972 ,  Y10S436/823 ,  Y10S530/866

    摘要： An improved homogeneous enzyme immunoassay process for quantitatively analyzing an antigen by determining the change in the enzymatic activity caused by a reaction between the antigen and an enzyme-labeled antibody. The antigen is reacted with an enzyme-labeled antibody, followed by the reaction with a second antibody capable of recognizing and binding to a different epitope and then with a third antibody capable of recognizing and binding to the second antibody. The enzymatic activity of the labeling enzyme is determined by a water-insoluble substrate. Using the water-insoluble substrate, steric hindrance is enhanced. A highly-sensitive analysis can be carried out by a simple operation even when the antigen has a molecular weight falling within an intermediate range, for example, a range of M.W. 10,000 to 70,000.

    摘要翻译： 一种改进的均匀酶免疫测定方法，用于通过测定由抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析抗原。 使抗原与酶标记的抗体反应，然后与能够识别和结合不同表位的第二抗体反应，然后与能够识别和结合第二抗体的第三抗体反应。 标记酶的酶活性由水不溶性底物决定。 使用水不溶性底物，空间位阻增强。 即使当抗原具有分子量落在中间范围内时，也可以通过简单的操作进行高灵敏度分析，例如，10,000至70000MW的范围。

    Measurement chip for surface plasmon resonance biosensor
    9.
    发明授权
    Measurement chip for surface plasmon resonance biosensor 有权
    表面等离子体共振生物传感器测量芯片

    公开(公告)号：US06726881B2

    公开(公告)日：2004-04-27

    申请号：US10230340

    申请日：2002-08-29

    申请人： Hiroshi Shinoki ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Osamu Seshimoto

    IPC分类号： G01N2175

    CPC分类号： G01N21/553 ,  G01N33/54393 ,  G01N33/553

    摘要： The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.

    摘要翻译： 本发明提供了一种用于表面等离子体共振生物传感器的测量芯片，其包括透明基板，位于透明基板上的金属膜和固定在金属膜上的有机硅膜，其中有机硅膜固定在金属上 膜通过能够与金属表面上的原子结合的官能团。 本发明的生物传感器用测量芯片可以容易地制造。 使用本发明的测量芯片，即使只固定少量的生理活性物质，也能够以良好的灵敏度测定目标物质。

    Immunoassay element containing a pulverized water-insoluble polysaccharide and process for immunoassay
    10.
    发明授权
    Immunoassay element containing a pulverized water-insoluble polysaccharide and process for immunoassay 失效
    含有粉碎水不溶性多糖的免疫测定元件和免疫测定方法

    公开(公告)号：US5547848A

    公开(公告)日：1996-08-20

    申请号：US393240

    申请日：1995-02-23

    申请人： Hiroshi Shinoki ,  Toshikage Hiraoka ,  Masashi Ogawa

    发明人： Hiroshi Shinoki ,  Toshikage Hiraoka ,  Masashi Ogawa

    IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/558 ,  G01N33/535 ,  G01N33/548

    CPC分类号： G01N33/54386 ,  G01N33/558

    摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity. When the ligand is a low molecular weight antigen, competitive reactions between the ligand, enzyme-labelled antibody and conjugate of the antigen and high molecular weight compound are utilized. When the ligand is a macromolecular antigen, a reaction between the ligand and an enzyme-labelled antibody is utilized directly. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer for detecting the thus formed diffusible material. The non-diffusible substrate is composed of a pulverized insoluble polysaccharide. The reagent layer may further contain a fragmenting enzyme for further fragmenting the non-diffusible material. Also provided are processes for quantitatively analyzing both of low molecular weight and macromolecular antigens contained in any samples by the use of the immunoassay elements of the invention.

    摘要翻译： 用于通过确定酶活性的变化定量分析配体的免疫测定元件。 当配体是低分子量抗原时，配体，酶标记抗体与抗原结合物和高分子量化合物之间的竞争性反应被利用。 当配体是大分子抗原时，直接使用配体和酶标记的抗体之间的反应。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和用于检测由此形成的可扩散材料的试剂层。 非扩散性基材由粉碎的不溶性多糖组成。 试剂层可以进一步包含用于进一步碎裂非扩散材料的碎裂酶。 还提供了通过使用本发明的免疫测定元件定量分析任何样品中所含的低分子量和大分子抗原的方法。