摘要:
A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要:
A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要:
A microfluidic device which electrochemically regulates a pH of a fluid, and a method of regulating a pH of a fluid using the microfluidic device, include a chamber which includes a cathode formed of a metal adsorbing hydrogen gas, and an anode formed of a metal having a higher standard oxidation potential than, and does not react with, water.
摘要:
Disclosed herein are a method of fabricating a microfluidic device, and a microfluidic device fabricated by the method. The method includes coating an adhesive material on a first substrate having a fluid port to form an adhesive layer thereon, arranging a second substrate having a microstructure formed therein with the surface of the first substrate on which the adhesive layer is formed, such that the fluid port and the microstructure correspond to each other, and heating the substrates at about 50 to about 180 degrees Celsius to bind the first substrate to the second substrate.
摘要:
A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要:
Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.
摘要:
A method of separating microorganisms from a sample includes introducing a sample into an apparatus which controls a concentration of at least one salt, the apparatus includes a reaction chamber defined between a cation exchange membrane and an anion exchange membrane, a first electrode chamber defined between the anion exchange membrane and a first electrode, the first electrode chamber containing a first ion exchange medium, a second electrode chamber defined between the cation exchange membrane and a second electrode, the second electrode chamber containing a second ion exchange medium, applying a voltage between the first electrode and the second electrode to electrodialyze the sample in the reaction chamber and reduce the concentration of the at least one salt in the sample and allowing the sample having the reduced concentration of the at least one salt to contact a microorganism capturing means.
摘要:
Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.
摘要:
A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.
摘要:
An ion exchangeable mixture containing a polymeric compound consisting of an ion exchange resin, an acrylamide mixture containing at least one bisacrylamide and at least one acrylamide, and a copolymer obtained by reacting the polymeric compound with the acrylamide mixture, and a method of producing the same are provided. The ion exchangeable membrane produced by using the ion exchangeable mixture has significantly smaller electric resistance than conventional ion exchangeable membranes, and has excellent selective permeability because the ion exchangeable membrane is electrically charged. The ion exchangeable membrane can be produced under very mild production conditions, and thus can be produced very easily. Furthermore, the ion exchangeable membrane can be also formed into film during a crosslinking reaction in a solvent of water, and thus is advantageous in that the ion exchangeable membrane can be freely produced into desired sizes, shapes and forms.