公开(公告)号：US20220323300A1

公开(公告)日：2022-10-13

申请号：US17639973

申请日：2020-09-09

申请人： James DEE ,  George GAGNE ,  Daniel LAMOTHE ,  EMD Millipore Corporation

发明人： Shannon Cleveland ,  Marina Varlamova ,  John Saragosa ,  David DeCoste ,  Benjamin Cacace ,  Daniel Lamothe ,  George Gagne ,  James Dee ,  Wilson Moya ,  Stefano Berti Perez

IPC分类号： A61J1/10 ,  C12M1/00 ,  C12M1/34 ,  B32B27/12 ,  B32B27/32 ,  B32B3/30 ,  B32B5/02 ,  G01M3/32

摘要： A biocontainer having a first film, the film having an interior and exterior side; articulating elements disposed on or within the first film, the articulating elements comprising at least one a folded hinge, a sealed joint, a thinned pathway, a bowed path, an embedded polymeric or metallic cylindrical fiber or rod; and a second film, optionally comprising articulating elements, joined to the first film, to form a biocontainer having a closed volume, wherein the articulating elements permit the biocontainer to expand and collapse along the articulating elements.

公开(公告)号：US09816901B2

公开(公告)日：2017-11-14

申请号：US14438961

申请日：2013-12-13

申请人： EMD Millipore Corporation

发明人： Benjamin Cacace

IPC分类号： G01N30/00 ,  B01L3/00 ,  G01N1/34 ,  G01N1/40 ,  C07K1/14 ,  C07K1/36 ,  B01J39/26 ,  B01J41/20 ,  B01J47/022 ,  B01J47/014 ,  B01J47/127

CPC分类号： G01N1/34 ,  B01J39/26 ,  B01J41/20 ,  B01J47/014 ,  B01J47/022 ,  B01J47/127 ,  B01L3/505 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/123 ,  C07K1/14 ,  C07K1/36 ,  G01N1/405

摘要： The invention relates to a liquid sample preparation device such as a disposable, collapsible, flexible polymeric bag containing an adsorptive curtain of a functionalized shaped polymeric fiber bed for the direct capture of biomolecules from liquid samples. The functionalized shaped polymeric fiber bed includes fibrillated, ridged or winged-shaped fiber structures that significantly increases the surface area of the fiber resulting in enhanced separation, retention and/or purification of liquid samples containing biomolecules of interest as the liquid samples contact the adsorptive curtain of functionalized shaped fibers. Liquid samples include unclarified liquid feeds or other liquids containing one or more biomolecules of interest, including, but not limited to vaccines, recombinant proteins, cells, stem cells, monoclonal antibodies (mAbs), proteins, antibody, peptides, oligopeptides, nucleic acids, oligonucleotides, RNA, DNA, oligosaccharides and polysaccharides.

公开(公告)号：US20170165638A1

公开(公告)日：2017-06-15

申请号：US15323471

申请日：2015-08-19

申请人： EMD Millipore Corporation

发明人： John Paul Amara ,  John Boyle ,  David Yavorsky ,  Benjamin Cacace

IPC分类号： B01J20/285 ,  B01D15/32 ,  B01D15/38 ,  B01J47/127 ,  B01J20/28 ,  B01J39/19 ,  B01J41/13 ,  B01D15/36 ,  C12N7/00

摘要： Chromatography media including a high surface area thermoplastic porous nanofiber and an ion-exchange ligand functionality on the surface of the fiber. The porous nanofibers display a convoluted structure that is comprised of discrete bundles of highly entangled nanofibrils that may be fibrillated or ridged. The porous fibers can be prepared through the extraction of a dissolvable mineral or polymeric porogen that is embedded into the fiber during its manufacture in a melt extrusion process.

公开(公告)号：US10449517B2

公开(公告)日：2019-10-22

申请号：US15323471

申请日：2015-08-19

申请人： EMD Millipore Corporation

发明人： John Paul Amara ,  John Boyle ,  David Yavorsky ,  Benjamin Cacace

IPC分类号： B01J20/285 ,  B01D15/32 ,  B01J20/26 ,  B01J20/28 ,  B01J20/281 ,  B01J20/32 ,  C07K1/16 ,  B01D15/36 ,  B01D15/38 ,  B01J39/19 ,  B01J47/127 ,  B01J41/13 ,  C12N7/00 ,  B01J39/26 ,  B01J41/20 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22

摘要： Chromatography media including a high surface area thermoplastic porous nanofiber and an ion-exchange ligand functionality on the surface of the fiber. The porous nanofibers display a convoluted structure that is comprised of discrete bundles of highly entangled nanofibrils that may be fibrillated or ridged. The porous fibers can be prepared through the extraction of a dissolvable mineral or polymeric porogen that is embedded into the fiber during its manufacture in a melt extrusion process.

公开(公告)号：US20150292991A1

公开(公告)日：2015-10-15

申请号：US14438961

申请日：2013-12-13

申请人： EMD MILLIPORE CORPORATION

发明人： Benjamin Cacace

IPC分类号： G01N1/34

CPC分类号： G01N1/34 ,  B01J39/26 ,  B01J41/20 ,  B01J47/014 ,  B01J47/022 ,  B01J47/127 ,  B01L3/505 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/123 ,  C07K1/14 ,  C07K1/36 ,  G01N1/405

摘要： The invention relates to a liquid sample preparation device such as a disposable, collapsible, flexible polymeric bag containing an adsorptive curtain of a functionalized shaped polymeric fiber bed for the direct capture of biomolecules from liquid samples. The functionalized shaped polymeric fiber bed includes fibrillated, ridged or winged-shaped fiber structures that significantly increases the surface area of the fiber resulting in enhanced separation, retention and/or purification of liquid samples containing biomolecules of interest as the liquid samples contact the adsorptive curtain of functionalized shaped fibers. Liquid samples include unclarified liquid feeds or other liquids containing one or more biomolecules of interest, including, but not limited to vaccines, recombinant proteins, cells, stem cells, monoclonal antibodies (mAbs), proteins, antibody, peptides, oligopeptides, nucleic acids, oligonucleotides, RNA, DNA, oligosaccharides and polysaccharides.

摘要翻译： 本发明涉及一种液体样品制备装置，例如一次性可折叠的柔性聚合物袋，其包含用于从液体样品直接捕获生物分子的官能化成形聚合物纤维床的吸附帘。 功能化的聚合物纤维床包括原纤化的，脊状的或翼状的纤维结构，其显着增加纤维的表面积，导致当液体样品接触吸附窗帘时，含有生物分子的液体样品的分离，保留和/或纯化增强 的功能化成形纤维。 液体样品包括未澄清的液体进料或含有一种或多种感兴趣的生物分子的其它液体，包括但不限于疫苗，重组蛋白，细胞，干细胞，单克隆抗体（mAb），蛋白质，抗体，肽，寡肽，核酸， 寡核苷酸，RNA，DNA，寡糖和多糖。