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1.发明授权Phytases, nucleic acids encoding them and methods for making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07824895B2
公开(公告)日：2010-11-02
申请号：US11933174
申请日：2007-10-31
申请人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
发明人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
IPC分类号： C07H21/00 , C12N15/00 , C12N5/10 , C12N1/21 , C12P21/00 , C12N9/16 , C07K14/00 , C12Q1/68 , C12Q1/44
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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公开(公告)号：US07432098B2
公开(公告)日：2008-10-07
申请号：US11056354
申请日：2005-02-11
申请人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
发明人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
IPC分类号： C12N9/16 , C12N11/02 , C12Q1/34 , A61K38/46 , C07K14/00 , A23J1/00 , C12P21/00 , C12N1/21 , C12N5/10 , C07H21/00
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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3.发明授权Phytases, nucleic acids encoding them and methods of making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07432097B2
公开(公告)日：2008-10-07
申请号：US10933115
申请日：2004-09-01
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
IPC分类号： C12N11/00 , C12N9/16 , A61K38/46 , C07K14/00 , C12N15/00 , C12N1/21 , C12N5/10 , C12P21/00 , C12Q1/44 , C07H21/00
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US07078035B2
公开(公告)日：2006-07-18
申请号：US10156660
申请日：2002-05-24
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
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5.发明授权Amylases, nucleic acids encoding them and methods for making and using them 有权淀粉酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07560126B2
公开(公告)日：2009-07-14
申请号：US10385305
申请日：2003-03-06
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin A. Gray , Janne S. Kerovuo , Malgorzata Slupska , Nelson R. Barton , Eileen O'Donoghue , Eric J. Mathur , Jay M. Short
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin A. Gray , Janne S. Kerovuo , Malgorzata Slupska , Nelson R. Barton , Eileen O'Donoghue , Eric J. Mathur , Jay M. Short
CPC分类号： C12N9/2451 , A21D8/042 , A23K10/14 , A23K20/189 , A23L29/06 , A23L29/35 , C11D3/386 , C12C5/004 , C12N9/2417 , C12Y302/01001 , C12Y302/01002
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。
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公开(公告)号：US06855365B2
公开(公告)日：2005-02-15
申请号：US09866379
申请日：2001-05-24
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , Eric J. Mathur
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , Eric J. Mathur
IPC分类号： A01H5/00 , A01K67/027 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/16 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/20 , A23L1/211 , A23L1/30 , A61K38/00 , C07K16/40 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/16 , C12N15/09 , C12P21/08 , C12Q1/42 , G01N33/53 , G01N33/566 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/34
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US07078504B2
公开(公告)日：2006-07-18
申请号：US10000997
申请日：2001-11-30
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.
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8.发明授权Dehalogenases, nucleic acids encoding them and methods for making and using them 有权脱卤素酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US09453211B2
公开(公告)日：2016-09-27
申请号：US13442514
申请日：2012-04-09
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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9.发明申请Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them 有权脱卤素酶，编码它们的核酸及其制备和使用方法公开(公告)号：US20100112666A1
公开(公告)日：2010-05-06
申请号：US12683906
申请日：2010-01-07
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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公开(公告)号：US07671189B2
公开(公告)日：2010-03-02
申请号：US11418828
申请日：2006-05-05
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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