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1.发明申请PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权PHYTASES，NUCLEIC ACID ENCODING THEM AND METHODS FOR MAKING AND USING THEM公开(公告)号：US20090062139A1
公开(公告)日：2009-03-05
申请号：US11933174
申请日：2007-10-31
申请人： Jay M. SHORT , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
发明人： Jay M. SHORT , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
IPC分类号： C40B30/04 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N1/15 , C12N1/19 , C40B40/08 , C12N15/00 , C12Q1/68 , C12P21/06
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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公开(公告)号：US07432098B2
公开(公告)日：2008-10-07
申请号：US11056354
申请日：2005-02-11
申请人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
发明人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
IPC分类号： C12N9/16 , C12N11/02 , C12Q1/34 , A61K38/46 , C07K14/00 , A23J1/00 , C12P21/00 , C12N1/21 , C12N5/10 , C07H21/00
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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3.发明授权Phytases, nucleic acids encoding them and methods for making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07824895B2
公开(公告)日：2010-11-02
申请号：US11933174
申请日：2007-10-31
申请人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
发明人： Jay M. Short , Kevin A. Gray , Nelson R. Barton , James B. Garrett , Eileen O'Donoghue , Dan E. Robertson
IPC分类号： C07H21/00 , C12N15/00 , C12N5/10 , C12N1/21 , C12P21/00 , C12N9/16 , C07K14/00 , C12Q1/68 , C12Q1/44
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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4.发明授权Phytases, nucleic acids encoding them and methods of making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07432097B2
公开(公告)日：2008-10-07
申请号：US10933115
申请日：2004-09-01
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
IPC分类号： C12N11/00 , C12N9/16 , A61K38/46 , C07K14/00 , C12N15/00 , C12N1/21 , C12N5/10 , C12P21/00 , C12Q1/44 , C07H21/00
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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5.发明申请Chimeric Cannulae Proteins, Nucleic Acids Encoding Them And Methods For Making And Using Them 审中-公开嵌合插管蛋白，编码它们的核酸和制造和使用它们的方法公开(公告)号：US20080274155A1
公开(公告)日：2008-11-06
申请号：US10593846
申请日：2005-03-24
申请人： Nelson R. Barton , Eileen O'Donoghue , Ryan Short , Gerhard Frey , David Weiner , Dan E. Robertson , Steven Briggs , Paul Zorner
发明人： Nelson R. Barton , Eileen O'Donoghue , Ryan Short , Gerhard Frey , David Weiner , Dan E. Robertson , Steven Briggs , Paul Zorner
IPC分类号： A61F2/00 , C07K14/195 , C12N9/00 , C12N9/40 , C12N9/10 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N1/19 , C12N1/15 , A01K67/027 , A01H5/00 , C12P1/00 , A61K38/16 , A01K1/015 , B32B9/04 , A61K39/00 , A61K39/395 , A61K31/70 , C12N5/06 , A61K35/12
CPC分类号： C07K14/195 , C07K2319/00 , Y10T428/249921
摘要： The invention provides chimeric cannulae polypeptides and nanotubules and methods for making and using them. In one aspect, the invention provides compositions and methods for the identification, separation or synthesis of proteins or ligands. In one aspect, the invention provides compositions and methods for making and using nanotubules. In one aspect, the invention provides compositions and methods for the selection and purification of chiral compositions from racemic mixtures. In one aspect, the chimeric proteins and polymers (e.g., nanotubules, tubules, bundles, balls, fibers, filaments, sheets, threads, textiles) of the invention comprise a detectable moiety, e.g., a fluorescent protein. In one aspect, the invention provides a flame retardant or heat resistant device comprising a sheeting, a covering, a coating or an adhesive comprising a chimeric protein of the invention.
摘要翻译： 本发明提供了嵌合插管多肽和纳米管，以及制备和使用它们的方法。 一方面，本发明提供用于鉴定，分离或合成蛋白质或配体的组合物和方法。 一方面，本发明提供了制备和使用纳米管的组合物和方法。 一方面，本发明提供了用于从外消旋混合物中选择和纯化手性组合物的组合物和方法。 在一个方面，本发明的嵌合蛋白质和聚合物（例如纳米管，小管，束，球，纤维，丝，片，丝，纺织品）包含可检测部分，例如荧光蛋白。 一方面，本发明提供一种阻燃或耐热装置，其包含片剂，覆盖物，包衣或包含本发明的嵌合蛋白质的粘合剂。
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公开(公告)号：US07078504B2
公开(公告)日：2006-07-18
申请号：US10000997
申请日：2001-11-30
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.
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7.发明授权Dehalogenases, nucleic acids encoding them and methods for making and using them 有权脱卤素酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US09453211B2
公开(公告)日：2016-09-27
申请号：US13442514
申请日：2012-04-09
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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8.发明申请Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them 有权脱卤素酶，编码它们的核酸及其制备和使用方法公开(公告)号：US20100112666A1
公开(公告)日：2010-05-06
申请号：US12683906
申请日：2010-01-07
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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公开(公告)号：US07671189B2
公开(公告)日：2010-03-02
申请号：US11418828
申请日：2006-05-05
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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10.发明申请Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them 有权脱卤素酶，编码它们的核酸及其制备和使用方法公开(公告)号：US20130115675A1
公开(公告)日：2013-05-09
申请号：US13442514
申请日：2012-04-09
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
IPC分类号： C12N9/14
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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