公开(公告)号：US20060155195A1

公开(公告)日：2006-07-13

申请号：US11361256

申请日：2006-02-24

申请人： John Maier ,  Shona Stewart ,  Jeffrey Cohen ,  Matthew Nelson

发明人： John Maier ,  Shona Stewart ,  Jeffrey Cohen ,  Matthew Nelson

IPC分类号： A61B6/00 ,  G01J3/44 ,  A61B18/18 ,  G01J3/40 ,  A61B5/00 ,  A61B5/05

CPC分类号： G01N21/65 ,  A61B5/0075 ,  A61B5/0084 ,  G01N2021/6484 ,  G01N2021/656 ,  G01N2201/08

摘要： A method and system to differentiate a tissue margins during various medical procedures. A region containing a biological tissue is irradiated, with a substantially monochromatic light. Raman spectroscopic data is obtained from the irradiated region. A boundary between a neoplastic portion and a non-neoplastic portion, in the region containing the biological tissue, is differentiated by evaluating the Raman spectroscopic data for at least one Raman spectroscopic value characteristic of either the neoplastic portion or the non-neoplastic portion. The neoplastic portion is selected for physical manipulation based on the differentiation of the boundary between the neoplastic portion and the non-neoplastic portion.

公开(公告)号：US20120150028A1

公开(公告)日：2012-06-14

申请号：US13316703

申请日：2011-12-12

申请人： Patrick J. Treado ,  John S. Maier ,  Shona Stewart ,  Jeffrey Cohen ,  Matthew Nelson

发明人： Patrick J. Treado ,  John S. Maier ,  Shona Stewart ,  Jeffrey Cohen ,  Matthew Nelson

IPC分类号： A61B6/00

CPC分类号： A61B5/0075 ,  A61B5/0071 ,  A61B5/0084 ,  A61B5/4064 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/44 ,  G01N21/65 ,  G01N2021/6484 ,  G01N2201/08

摘要： System and method for differentiating tissue margins in a biological sample using pulsed laser excitation and time-gated detection. A region containing a biological tissue is irradiated with substantially monochromatic pulsed laser light to thereby produce Raman scattered photons. The Raman scattered photons are detected using time-gated detection to thereby obtain a Raman spectroscopic image from the irradiated region characteristic of either a neoplastic portion or a non-neoplastic portion of the region containing the biological tissue. A boundary between a neoplastic portion and a non-neoplastic portion is differentiated and the boundary location in the Raman spectroscopic image is displayed.

摘要翻译： 使用脉冲激光激发和时间门控检测来区分生物样品中组织边缘的系统和方法。 用基本单色的脉冲激光照射含有生物组织的区域，从而产生拉曼散射的光子。 使用时间门控检测来检测拉曼散射的光子，从而从包含生物体组织的区域的肿瘤部位或非肿瘤部位的特征的照射区域获得拉曼光谱图像。 肿瘤部位和非肿瘤部位之间的边界被区分，并且显示拉曼光谱图像中的边界位置。

公开(公告)号：US20070178067A1

公开(公告)日：2007-08-02

申请号：US11598248

申请日：2006-11-09

申请人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

发明人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

IPC分类号： A61K48/00 ,  C12Q1/00 ,  G06F19/00

CPC分类号： G01N33/5005 ,  G01N15/1459 ,  G01N21/65 ,  G01N33/574 ,  G01N33/57407 ,  G01N2021/656

摘要： A method and system of differentially manipulating cells where the cells, suspended in a fluid, are irradiated with substantially monochromatic light. A Raman data set is obtained from the irradiated cells and where the data set is characteristic of a disease status. The data set is assessed to identify diseased cells. A Raman chemical image of the irradiated cells is also obtained. Based on the assessment and the Raman chemical image, the fluid in which the cells are suspended is differentially manipulated. The diseased cells are directed to a first location and other non-diseased cells are directed to a second location as part of the differential manipulation. The diseased cells may be treated with a physical stress, a chemical stress, and a biological stress and then returned to a patient from whom the diseased cells were obtained prior to the irradiation.

摘要翻译： 一种差异操纵细胞的方法和系统，其中悬浮在流体中的细胞用基本上单色的光照射。 从照射的细胞获得拉曼数据集，其中数据集是疾病状态的特征。 评估数据集以鉴定患病细胞。 还获得了照射的细胞的拉曼化学图像。 基于评估和拉曼化学图像，细胞悬浮的流体被差异地操纵。 病变细胞被引导到第一位置，并且其他非病变细胞被引导到第二位置作为差异操纵的一部分。 患病细胞可以用物理应力，化学应力和生物应激治疗，然后返回到在照射之前从其获得患病细胞的患者。

公开(公告)号：US20050277816A1

公开(公告)日：2005-12-15

申请号：US11000591

申请日：2004-11-30

申请人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

发明人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

IPC分类号： A61B5/00

CPC分类号： A61B5/0084 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/415 ,  A61B5/417 ,  A61B5/418 ,  A61B5/4519 ,  G01N21/65

摘要： Raman molecular imaging is used to differentiate between normal and diseased cells or tissue. For instance benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view.

摘要翻译： 拉曼分子成像用于区分正常和病变细胞或组织。 例如，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾脏，乳腺和结肠，以及非上皮组织，如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。

公开(公告)号：US20120078524A1

公开(公告)日：2012-03-29

申请号：US13136366

申请日：2011-07-29

申请人： Shona Stewart ,  Jeffrey Cohen ,  Amy Drauch ,  John Maier

发明人： Shona Stewart ,  Jeffrey Cohen ,  Amy Drauch ,  John Maier

IPC分类号： G01N33/48 ,  G01N21/64 ,  G01J3/44

CPC分类号： A61B5/0059 ,  A61B5/417 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/32 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/6458 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  G02B21/16 ,  G02B21/365 ,  G06F19/325 ,  G16H50/70

摘要： A system and method for determining a diagnosis of a test biological sample. A system comprising a first illumination source to illuminate a sample, a first detector for generating a fluorescence data set of said sample, a means for determining a region of interest, a second illumination source to illuminate said region of interest, a second detector to generate a Raman data set of said region of interest, and a means for determining a diagnosis of said sample. A method comprising illuminating a sample, generating a fluorescence data set of said sample, and assessing the fluorescence data set to identify a region of interest, illuminating a region of interest, and generating Raman data set. This Raman data set may be assessed to determine a diagnosis of the sample. A diagnosis may include a metabolic state, a clinical outcome, a disease progression, a disease state, and combinations thereof.

摘要翻译： 用于确定测试生物样品的诊断的系统和方法。 一种系统，包括用于照亮样品的第一照明源，用于产生所述样品的荧光数据集的第一检测器，用于确定感兴趣区域的装置，用于照亮所述感兴趣区域的第二照明源，产生 所述感兴趣区域的拉曼数据集，以及用于确定所述样本的诊断的装置。 一种方法，包括照亮样品，产生所述样品的荧光数据集，以及评估所述荧光数据集以识别感兴趣区域，照亮感兴趣区域，以及产生拉曼数据集。 可以评估该拉曼数据集以确定样品的诊断。 诊断可以包括代谢状态，临床结果，疾病进展，疾病状态及其组合。

公开(公告)号：US07956996B2

公开(公告)日：2011-06-07

申请号：US12206500

申请日：2008-09-08

申请人： John Maier ,  Jeffrey Cohen ,  Shona Stewart

发明人： John Maier ,  Jeffrey Cohen ,  Shona Stewart

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/02 ,  A61B5/0059 ,  A61B5/417 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/36 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/65 ,  G01N33/57415 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  Y02A90/26

摘要： A system and method to provide a diagnosis of the breast disease state of a test breast sample. A database containing a plurality of reference Raman data sets is provided where each reference Raman data set has an associated known breast sample and an associated known breast disease state. A test breast sample is irradiated with substantially monochromatic light to generate scattered photons resulting in a test Raman data set. The test Raman data set is compared to the plurality of reference Raman data sets using a chemometric technique. Based on the comparison, a diagnosis of a breast disease state of the test breast sample is provided. The breast disease state includes invasive ductal carcinoma or invasive lobular carcinoma disease state.

摘要翻译： 一种用于提供测试乳房样品的乳腺疾病状态诊断的系统和方法。 提供了包含多个参考拉曼数据集的数据库，其中每个参考拉曼数据集具有相关联的已知乳房样本和相关联的已知乳腺疾病状态。 用基本上单色的光照射测试乳样品以产生散射的光子，得到测试拉曼数据集。 使用化学计量技术将测试拉曼数据集与多个参考拉曼数据集进行比较。 基于比较，提供了测试乳房样品的乳腺疾病状态的诊断。 乳腺疾病状态包括浸润性导管癌或侵袭性小叶癌疾病状态。

公开(公告)号：US20120310538A1

公开(公告)日：2012-12-06

申请号：US13450579

申请日：2012-04-19

申请人： Shona Stewart ,  Arny Drauch ,  Jeffrey Cohen ,  John Maier

发明人： Shona Stewart ,  Arny Drauch ,  Jeffrey Cohen ,  John Maier

IPC分类号： G01J3/46 ,  G06F19/00 ,  G01J5/02

CPC分类号： G06K9/00127

摘要： The present disclosure provides for a system and method for analyzing biological samples to thereby provide a diagnosis. A system may comprise an illumination source, a filter and a detector configured to generate at least one of: a visible data set representative of a biological sample, a SWIR data set representative of a biological sample, and combinations thereof. A method may comprise illuminating a biological sample to generate a plurality of photons, filtering a said plurality of interacted photons, detecting

摘要翻译： 本公开提供了用于分析生物样品从而提供诊断的系统和方法。 系统可以包括照明源，滤光器和检测器，其被配置为产生代表生物样品的可见数据集，表示生物样品的SWIR数据集及其组合中的至少一个。 一种方法可以包括照射生物样品以产生多个光子，过滤所述多个相互作用的光子，检测

公开(公告)号：US20100262378A1

公开(公告)日：2010-10-14

申请号：US12755463

申请日：2010-06-23

申请人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

发明人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

IPC分类号： G06F19/00 ,  G01J3/44 ,  G06K9/62

CPC分类号： A61B5/0084 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/415 ,  A61B5/417 ,  A61B5/418 ,  A61B5/4519 ,  G01N21/65

摘要： A method for generating an image of a sample that is informative of the disease state of a cell in the sample. A sample including the cell is irradiated with monochromatic light. The Raman scattered light is assessed. A digital brightfield image of the Raman scattered light is generated and combined with the Raman scattered light emitted by the cell whereby the Raman scattered light is informative of the disease state of the cell in the sample. The method can also be used to determine the metabolic activity of the cell, the inflammatory status of the cell and/or the infected status of the cell in the sample.

摘要翻译： 一种产生样品图像的方法，其用于提供样品中细胞的疾病状态。 用单色光照射包含电池的样品。 评估拉曼散射光。 产生拉曼散射光的数字明场图像，并与细胞发射的拉曼散射光组合，由此拉曼散射光提供样品中细胞的疾病状态。 该方法还可以用于确定细胞的代谢活性，细胞的炎性状态和/或样品中细胞的感染状态。

公开(公告)号：US20080319324A1

公开(公告)日：2008-12-25

申请号：US12206500

申请日：2008-09-08

申请人： John Maier ,  Shona Stewart ,  Jeffrey Cohen

发明人： John Maier ,  Shona Stewart ,  Jeffrey Cohen

IPC分类号： A61B6/00

CPC分类号： G01J3/02 ,  A61B5/0059 ,  A61B5/417 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/36 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/65 ,  G01N33/57415 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  Y02A90/26

摘要： A system and method to provide a diagnosis of the breast disease state of a test breast sample. A database containing a plurality of reference Raman data sets is provided where each reference Raman data set has an associated known breast sample and an associated known breast disease state. A test breast sample is irradiated with substantially monochromatic light to generate scattered photons resulting in a test Raman data set. The test Raman data set is compared to the plurality of reference Raman data sets using a chemometric technique. Based on the comparison, a diagnosis of a breast disease state of the test breast sample is provided. The breast disease state includes invasive ductal carcinoma or invasive lobular carcinoma disease state.

摘要翻译： 一种用于提供测试乳房样品的乳腺疾病状态诊断的系统和方法。 提供了包含多个参考拉曼数据集的数据库，其中每个参考拉曼数据集具有相关联的已知乳房样本和相关联的已知乳腺疾病状态。 用基本上单色的光照射测试乳房样品以产生散射光子，得到测试拉曼数据集。 使用化学计量技术将测试拉曼数据集与多个参考拉曼数据集进行比较。 基于比较，提供了测试乳房样品的乳腺疾病状态的诊断。 乳腺疾病状态包括浸润性导管癌或侵袭性小叶癌疾病状态。

公开(公告)号：US07990533B2

公开(公告)日：2011-08-02

申请号：US12834370

申请日：2010-07-12

申请人： John Maier ,  Shona Stewart ,  Jeffrey Cohen

发明人： John Maier ,  Shona Stewart ,  Jeffrey Cohen

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/10 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/417 ,  A61B5/7264 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/2823 ,  G01J3/32 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  G02B21/365 ,  G06F19/00 ,  G16H50/70

摘要： A system and method for determining at least one of: a disease state, a metabolic state, a clinical outcome, and a disease progression of a test renal or prostate sample. A test Raman data set is obtained from the sample wherein said test Raman data set may comprise at least one of a plurality of Raman spectra and a plurality of spatially accurate wavelength resolved Raman images. The test Raman data set is compared to a plurality of reference Raman data sets using a chemometric technique. For analysis of renal samples, each of these reference Raman data sets may have an associated known renal sample and an associated known metabolic state, clinical outcome, and/or disease progression. For analysis of prostate samples, each of these reference Raman data sets may have an associated known prostate sample and an associated known disease state, metabolic state, clinical outcome, and/or disease progression.

摘要翻译： 用于确定测试的肾或前列腺样品的疾病状态，代谢状态，临床结果和疾病进展中的至少一个的系统和方法。 从样品获得测试拉曼数据集，其中所述测试拉曼数据集可以包括多个拉曼光谱和多个空间上准确的波长分辨拉曼图像中的至少一个。 使用化学计量技术将测试拉曼数据集与多个参考拉曼数据集进行比较。 对于肾样品的分析，这些参考拉曼数据集中的每一个可以具有相关联的已知肾样品和相关联的已知代谢状态，临床结果和/或疾病进展。 对于前列腺样品的分析，这些参考拉曼数据集中的每一个可以具有相关联的已知前列腺样品和相关的已知疾病状态，代谢状态，临床结果和/或疾病进展。