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公开(公告)号：US20120058460A1
公开(公告)日：2012-03-08
申请号：US13222887
申请日：2011-08-31
申请人： Johnathan S. Coursey , Kenton C. Hasson , Sami Kanderian , Gregory H. Owen , Hongye Liang , Scott Corey , Brian Bean
发明人： Johnathan S. Coursey , Kenton C. Hasson , Sami Kanderian , Gregory H. Owen , Hongye Liang , Scott Corey , Brian Bean
CPC分类号： B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/143 , B01L2200/147 , B01L2400/082 , G01N33/582 , Y10T137/0318
摘要： The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
摘要翻译： 本发明在一个方面提供了使用温度依赖性荧光染料来控制s。的方法和系统。 在一些实施例中，本发明使用一种或多种技术来增强s塞的可视性，增强系统区分s's的能力，并且增强系统识别s the的位置的能力。
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公开(公告)号：US09861985B2
公开(公告)日：2018-01-09
申请号：US13222887
申请日：2011-08-31
申请人： Johnathan S. Coursey , Kenton C. Hasson , Sami Kanderian , Gregory H. Owen , Hongye Liang , Scott Corey , Brian Bean
发明人： Johnathan S. Coursey , Kenton C. Hasson , Sami Kanderian , Gregory H. Owen , Hongye Liang , Scott Corey , Brian Bean
CPC分类号： B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/143 , B01L2200/147 , B01L2400/082 , G01N33/582 , Y10T137/0318
摘要： The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
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3.发明授权公开(公告)号：US09114399B2
公开(公告)日：2015-08-25
申请号：US13223290
申请日：2011-08-31
申请人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
发明人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
CPC分类号： B01L3/502746 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2200/028 , B01L2200/10 , B01L2300/0663 , B01L2300/0816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0487 , C12Q1/686 , G01N21/77 , G01N35/08 , G01N35/1095 , G01N2021/7786 , C12Q2561/113 , C12Q2565/629
摘要： The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
摘要翻译： 本发明涉及用于在聚合酶链反应（PCR）和热熔体应用中实时处理核酸的系统和方法。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。
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公开(公告)号：US20120052560A1
公开(公告)日：2012-03-01
申请号：US13223290
申请日：2011-08-31
申请人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
发明人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
IPC分类号： C12M1/40
CPC分类号： B01L3/502746 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2200/028 , B01L2200/10 , B01L2300/0663 , B01L2300/0816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0487 , C12Q1/686 , G01N21/77 , G01N35/08 , G01N35/1095 , G01N2021/7786 , C12Q2561/113 , C12Q2565/629
摘要： The present invention relates generally to systems and methods for the rapid serial processing of multiple nucleic acid assays. More particularly, the present invention provides for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
摘要翻译： 本发明一般涉及用于多重核酸测定的快速连续加工的系统和方法。 更具体地说，本发明提供了在聚合酶链反应（PCR）和热熔体应用中核酸的实时处理。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。
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5.发明授权Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes 有权具有集成电阻加热器电极的微流体装置包括用于控制和测量这种加热器电极的温度的系统和方法公开(公告)号：US09527083B2
公开(公告)日：2016-12-27
申请号：US12165043
申请日：2008-06-30
CPC分类号： B01L7/525 , B01L3/5027 , B01L3/502715 , B01L7/52 , B01L2200/147 , B01L2300/0816 , B01L2300/1805 , B01L2300/1827 , B01L2300/1833 , C12Q1/686 , G01K7/16
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature each heater electrode by determining the resistance of each heater electrode.
摘要翻译： 本发明涉及用于控制具有多个微流体通道的集成薄膜器件的方法和装置。 在一个实施例中，提供了一种微流体装置，其包括具有多个微流体通道的微流体芯片和多个多路加热器电极，其中加热器电极是多路复用电路的一部分，其包括将加热器电极连接到电源 ，每个加热器电极与微流体通道中的一个相关联。 微流体装置还包括控制系统，其被配置为通过改变占空比来调节施加到每个加热器电极的功率，所述控制系统还被配置为通过确定每个加热器电极的电阻来确定每个加热器电极的温度。
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公开(公告)号：US20110048547A1
公开(公告)日：2011-03-03
申请号：US12825476
申请日：2010-06-29
CPC分类号： B01L7/52 , B01L3/502715 , B01L7/525 , B01L2200/14 , B01L2200/147 , B01L2300/0645 , B01L2300/1805 , B01L2300/1827 , C12Q3/00 , H05B1/02 , Y10T137/6606
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed resistive thermal detectors (RTDs). Each of the RTDs is associated with one of the microfluidic channels. The RTDs are connected to a power supply through individual electrodes and pairs of common electrodes. Adjacent RTDs may be driven with alternating polarities, and the current in the common electrodes may be minimized using a virtual ground circuit. The compact microfluidic device is capable of fast heating and highly precise thermal control. The compact microfluidic device is also capable using the RTDs to sense temperature without their heating capability.
摘要翻译： 本发明涉及用于控制具有多个微流体通道的集成薄膜器件的方法和装置。 在一个实施例中，提供了包括具有多个微流体通道的微流体芯片和多个多路复用电阻热检测器（RTD）的微流体装置。 每个RTD与微流体通道中的一个相关联。 RTD通过单独的电极和公共电极对连接到电源。 可以用交替极性驱动相邻的RTD，并且可以使用虚拟接地电路来使公共电极中的电流最小化。 紧凑的微流体装置能够快速加热和高度精确的热控制。 紧凑型微流体装置还能够使用RTD来感测温度而不加热能力。
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公开(公告)号：US09061278B2
公开(公告)日：2015-06-23
申请号：US12825476
申请日：2010-06-29
CPC分类号： B01L7/52 , B01L3/502715 , B01L7/525 , B01L2200/14 , B01L2200/147 , B01L2300/0645 , B01L2300/1805 , B01L2300/1827 , C12Q3/00 , H05B1/02 , Y10T137/6606
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed resistive thermal detectors (RTDs). Each of the RTDs is associated with one of the microfluidic channels. The RTDs are connected to a power supply through individual electrodes and pairs of common electrodes. Adjacent RTDs may be driven with alternating polarities, and the current in the common electrodes may be minimized using a virtual ground circuit. The compact microfluidic device is capable of fast heating and highly precise thermal control. The compact microfluidic device is also capable using the RTDs to sense temperature without their heating capability.
摘要翻译： 本发明涉及用于控制具有多个微流体通道的集成薄膜器件的方法和装置。 在一个实施例中，提供了包括具有多个微流体通道的微流体芯片和多个多路复用电阻热检测器（RTD）的微流体装置。 每个RTD与微流体通道中的一个相关联。 RTD通过单独的电极和公共电极对连接到电源。 可以用交替极性驱动相邻的RTD，并且可以使用虚拟接地电路来使公共电极中的电流最小化。 紧凑的微流体装置能够快速加热和高度精确的热控制。 紧凑型微流体装置还能够使用RTD来感测温度而不加热能力。
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8.发明申请公开(公告)号：US20100285571A1
公开(公告)日：2010-11-11
申请号：US12437775
申请日：2009-05-08
摘要： The invention relates to systems and methods for calibrating and using resistance temperature detectors. In one embodiment, the system includes a calibration circuit comprising a resistance temperature detector in a bridge circuit with at least one potentiometer, and a programmable gain amplifier coupled to the bridge circuit. Embodiments of the invention further comprise methods for calibrating the bridge circuit and the programmable gain amplifier for use with the resistance temperature detector and methods for determining the self heating voltage of the bridge circuit.
摘要翻译： 本发明涉及用于校准和使用电阻温度检测器的系统和方法。 在一个实施例中，该系统包括校准电路，该校准电路包括具有至少一个电位器的桥接电路中的电阻温度检测器和耦合到该桥接电路的可编程增益放大器。 本发明的实施例还包括用于校准桥接电路的方法和用于电阻温度检测器的可编程增益放大器以及用于确定桥接电路的自身发热电压的方法。
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9.发明授权Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes 有权具有集成电阻加热器电极的微流体装置包括用于控制和测量这种加热器电极的温度的系统和方法公开(公告)号：US08306773B2
公开(公告)日：2012-11-06
申请号：US12872068
申请日：2010-08-31
IPC分类号： G06F19/00
CPC分类号： G01K7/16 , B01L3/5027 , B01L7/525 , B01L2300/0816 , B01L2300/1827 , G01K3/10 , G06F17/12
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
摘要翻译： 本发明涉及用于控制具有多个微流体通道的集成薄膜器件的方法和装置。 在一个实施例中，提供了一种微流体装置，其包括具有多个微流体通道的微流体芯片和多个多路加热器电极，其中加热器电极是多路复用电路的一部分，其包括将加热器电极连接到电源 ，每个加热器电极与微流体通道中的一个相关联。 微流体装置还包括控制系统，其被配置为通过改变占空比来调节施加到每个加热器电极的功率，所述控制系统还被配置为通过确定每个加热器电极的电阻来确定每个加热器电极的温度。
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公开(公告)号：US20110077897A1
公开(公告)日：2011-03-31
申请号：US12872068
申请日：2010-08-31
CPC分类号： G01K7/16 , B01L3/5027 , B01L7/525 , B01L2300/0816 , B01L2300/1827 , G01K3/10 , G06F17/12
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
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