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    Method of producing a polyunsaturated fatty acid containing culture and polyunsaturated fatty acid containing oil using microorganisms
    2.
    发明授权
    Method of producing a polyunsaturated fatty acid containing culture and polyunsaturated fatty acid containing oil using microorganisms 失效
    使用微生物生产含有不饱和脂肪酸的培养物和含有多不饱和脂肪酸的油的方法

    公开(公告)号：US06461839B2

    公开(公告)日：2002-10-08

    申请号：US09749035

    申请日：2000-12-28

    申请人： Toshihiro Yokochi ,  Toro Nakahara ,  Masakazu Yamaoka ,  Ryuichiro Kurane

    发明人： Toshihiro Yokochi ,  Toro Nakahara ,  Masakazu Yamaoka ,  Ryuichiro Kurane

    IPC分类号： C12P764

    CPC分类号： C12P7/6427 ,  A23D9/00 ,  C11B1/00 ,  C12N1/10 ,  C12N1/26 ,  C12P7/6472 ,  C12R1/90

    摘要： Present invention provides a method to efficiently manufacture an oil-containing product including long chain polyunsaturated fatty acid, or a high added value oil containing long chain polyunsaturated fatty acids. The method comprises culturing a microorganism belonging to the Genus Labyrinthula. The Labyrinthula microorganism is cultured in a medium containing oil or fatty acid as a carbon source, and the produced long chain polyunsaturated fatty acid is recovered from the culture. With the present invention, it is possible to efficiently produce from the vegetable oil and the like, an oil containing product comprising long chain polyunsaturated fatty acids such as docosahexanoic acid having a carbon number of 20 or more, and two or more unsaturated bonds, or an oil containing the long chain polyunsaturated fatty acid. Further, the method of the present invention may be used to reform oil by converting the plant oil and like to high added value oil containing the long chain polyunsaturated fatty acid.

    摘要翻译： 本发明提供了一种有效制造含有长链多不饱和脂肪酸或含有长链多不饱和脂肪酸的高附加值油的含油产品的方法。 该方法包括培养属于Labyrinthula属的微生物。 将Labyrinthula微生物在含有油或脂肪酸作为碳源的培养基中培养，并从培养物中回收生成的长链多不饱和脂肪酸。 通过本发明，可以从植物油等高效地制造含有碳数为20以上的二十二碳六烯酸的长链多不饱和脂肪酸和两个以上的不饱和键的含油产品，或 含有长链多不饱和脂肪酸的油。 此外，本发明的方法可用于通过将植物油转化为含有长链多不饱和脂肪酸的高附加值油来改性油。

    Nucleic acid probe and novel method of assaying nucleic acid using the same
    3.
    发明授权
    Nucleic acid probe and novel method of assaying nucleic acid using the same 失效
    核酸探针和使用其测定核酸的新方法

    公开(公告)号：US07273700B2

    公开(公告)日：2007-09-25

    申请号：US10399407

    申请日：2002-03-27

    申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

    发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q2565/101 ,  C12Q2565/107 ,  C12Q2565/1015

    摘要： A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

    摘要翻译： 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸，该对荧光染料包括能够服用的荧光染料（供体染料） 作为能够作为受体染料的供体染料和荧光染料（受体染料），其中核酸探针具有这样的碱基序列并用荧光染料标记，使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。

    Novel nucleic acid probe and novel method of assaying nucleic acid using the same
    4.
    发明申请
    Novel nucleic acid probe and novel method of assaying nucleic acid using the same 失效
    新型核酸探针和使用其的新型核酸分析方法

    公开(公告)号：US20050048485A1

    公开(公告)日：2005-03-03

    申请号：US10399407

    申请日：2002-03-27

    申请人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

    发明人： Ryuichiro Kurane ,  Takahiro Kanagawa ,  Yoichi Kamagata ,  Masaki Torimura ,  Shinya Kurata ,  Kazutaka Yamada ,  Toyokazu Yokomaku

    IPC分类号： C12Q1/68 ,  G01N33/58 ,  C07H21/04

    CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q2565/101 ,  C12Q2565/107 ,  C12Q2565/1015

    摘要： A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

    摘要翻译： 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸，该对荧光染料包括能够服用的荧光染料（供体染料） 作为能够用作受体染料的供体染料和荧光染料（受体染料），其中核酸探针具有这样的碱基序列并用荧光染料标记，使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。

    Method for isolating a microbe
    7.
    发明授权
    Method for isolating a microbe 失效
    隔离微生物的方法

    公开(公告)号：US06391576B1

    公开(公告)日：2002-05-21

    申请号：US09517744

    申请日：2000-03-03

    申请人： Takayasu Tsuchida ,  Akira Manome ,  Ryuichiro Kurane

    发明人： Takayasu Tsuchida ,  Akira Manome ,  Ryuichiro Kurane

    IPC分类号： C12Q124

    CPC分类号： C12Q1/24

    摘要： The present invention provides a method for isolating a microbe whereby a sample of microbe cells is encapsulated in agarose gel particulates, wherein some of the particulates contain a single cell, and the other particulates contain more than one cell; incubating the particulates in nutritional and environmental conditions that enable the microbe contained in the sample solution that can grow on a plate of a plate culture method to grow in the agarose gel particulate; and isolating the particulates having single cells from the group of the particulates having more than one cell.

    摘要翻译： 本发明提供一种分离微生物的方法，其中将微生物细胞的样品包封在琼脂糖凝胶颗粒中，其中一些微粒包含单个细胞，而其它颗粒含有多于一个细胞; 在营养和环境条件下孵育颗粒物，使得能够在平板培养方法板上生长的样品溶液中包含的微生物在琼脂糖凝胶颗粒中生长; 并从具有多于一个电池的颗粒组中分离出具有单个电池的颗粒。

    Polysaccharide and a method of producing it
    8.
    发明授权
    Polysaccharide and a method of producing it 失效
    多糖及其生产方法

    公开(公告)号：US5378832A

    公开(公告)日：1995-01-03

    申请号：US94091

    申请日：1993-07-20

    申请人： Ryuichiro Kurane ,  Yasuhiro Nohata ,  Michio Shiomi ,  Shuichi Ishino ,  Akira Yotsuji ,  Hideki Murata ,  Seiji Sugimoto

    发明人： Ryuichiro Kurane ,  Yasuhiro Nohata ,  Michio Shiomi ,  Shuichi Ishino ,  Akira Yotsuji ,  Hideki Murata ,  Seiji Sugimoto

    IPC分类号： B01F17/56 ,  C08B37/00 ,  C12P19/04 ,  C12N1/20

    CPC分类号： C12P19/04 ,  C08B37/006 ,  Y10S435/829

    摘要： The present invention provides a polymer comprising a polysaccharide having excellent water absorption properties, moisture absorption properties, moisture retention properties and thickening properties. Said polysaccharide has the following properties: (A) the principal constituents of the sugar composition are rhamnose, fucose, glucose and glucuronic acid which are present in a molar ratio of (1-4):2:(1-8):(1-4); (B) elemental analysis (wt %):C: 36 .+-.3H: 7 .+-.1O: 56 .+-.4,containing 9-13% of crystalline water(C) solubility:slightly soluble in water; soluble in alkalies; insoluble in methanol, ethanol and acetone;(D) UV absorption spectrum:no absorption detected at 280 nm characteristic of proteins (peptides) or at 260 nm characteristic of nucleic acids; and(E) IR absorption spectrum:an absorption pattern characteristic of polysaccharides is observed near 800-1200.sup.-iSaid polysaccharide is produced by a fermentation method using a natural medium or a synthetic medium of Alcaligenes microorganism.

    摘要翻译： PCT No.PCT / JP92 / 00695 Sec。 371日期：1993年7月20日 102（e）日期1993年7月20日PCT提交1993年7月20日PCT公布。 公开号WO93 / 11163 日期：6月10日，1993。本发明提供了包含具有优异的吸水性，吸湿性，保湿性和增稠性的多糖的聚合物。 所述多糖具有以下性质：（A）糖组合物的主要成分是以（1-4）：2：（1-8）：（1）的摩尔比存在的鼠李糖，岩藻糖，葡萄糖和葡萄糖醛酸 -4）; （B）元素分析（重量％）：C：36 +/- 3 H：7 +/- 1 O：56 +/- 4，含有9-13％的结晶水（C）溶解度：微溶于水; 溶于碱; 不溶于甲醇，乙醇和丙酮; （D）紫外线吸收光谱：蛋白质（肽）的特征性或280nm特征性的核酸在280nm处未检测到吸收; 和（E）IR吸收光谱：在800-1200-i附近观察到多糖的吸收图案特征。所述多糖通过使用天然培养基或产碱杆菌微生物的合成培养基的发酵方法产生。

    Polysaccharide, and water absorbent, moisture absorbent or humectant and thickening agent chiefly made of the polysaccharide
    9.
    发明授权
    Polysaccharide, and water absorbent, moisture absorbent or humectant and thickening agent chiefly made of the polysaccharide 失效
    多糖和吸水剂，吸湿剂或保湿剂和增稠剂主要由多糖制成

    公开(公告)号：US5175279A

    公开(公告)日：1992-12-29

    申请号：US735633

    申请日：1991-07-25

    申请人： Ryuichiro Kurane ,  Tomoo Suzuki ,  Yasuhiro Nohata

    发明人： Ryuichiro Kurane ,  Tomoo Suzuki ,  Yasuhiro Nohata

    IPC分类号： C08B37/00 ,  C12P19/04

    CPC分类号： C12P19/04 ,  C08B37/006 ,  Y10S435/829

    摘要： A polysaccharide (Biopolymer B-16) being produced by cultivating Alcaligenes latus strain B-16 (FERM-2015) and having at least one function selected from water absorption, moisture absorption, humectant capability, thickening capability, suspension stability, emulsion stability and dispersant capability along with high biodegradability and which can be used without creating any environmental hazard such as secondary pollution, said Biopolymer B-16 consisting essentially of rhamnose, fucose, glucose, mannose and glucuronic acid which are present in a molar ratio of(1-10):(2-10):(4-20):(1):(1-5).

    摘要翻译： 通过培养产碱杆菌菌株B-16（FERM-2015）并具有至少一种选自吸水性，吸湿性，湿润性能，增稠能力，悬浮稳定性，乳液稳定性和分散剂的功能而生产的多糖（Biopolymer B-16） 所述生物聚合物B-16主要由鼠李糖，岩藻糖，葡萄糖，甘露糖和葡萄糖醛酸组成，其摩尔比为（1-10） ）:( 2-10）：（4-20）:( 1）：（1-5）。