摘要:
The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.
摘要:
The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.
摘要:
The present invention provides apparatus (1) for conducting sequential nucleic acid amplification reactions. The apparatus (1) comprises a platform (2) having a sample compartment (5) and a plurality of reaction compartments (10). The sample compartment (5) is adapted to receive a fluid sample for conducting a first amplification reaction and the platform (2) is adapted to substantially evenly distribute the fluid sample into the plurality of reaction compartments (10) for conducting second amplification reactions. The present invention also extends to a kit, a method and a device for conducting sequential nucleic acid amplification reactions.
摘要:
The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.
摘要:
A process for producing an enzyme-resistant starch uses an aqueous feed composition that comprises (i) starch that contains at least about 50% by weight amylose, (ii) water, and (iii) alcohol. The concentration of starch in the feed composition is between about 5% and about 50% by weight, and the pH of the feed composition is between about 3.5 and about 6.5. In a first heating step, the feed composition is heated to a temperature between about 130-170° C. for about 0.1-3.0 hours. The feed composition is cooled to a temperature between about 4-70° C. for about 0.1-6.0 hours. In a second heating step, the feed composition is heated to a temperature between about 110-150° C. for about 0.1-10.0 hours. The starch is separated from the majority of the water and alcohol and is dried.
摘要:
The invention relates to improved methods of amplifying and optionally quantifying and/or identifying a plurality of selected nucleic acid molecules from a pool of nucleic acid molecules. A first round of multiplex amplification used where the amplification reaction is allowed to proceed to a point prior to that at which significant competition between amplicons for reaction components has occurred. This is the followed by a second round of amplification that typically includes a fluorescent reporter to allow for each of the selected nucleic acid sequences to be quantified. The methods are useful for the amplification and quantification of nucleic acids from a variety of sources, such as gene expression products, whereby many such products may be amplified and quantified from very limited samples and from degraded archival samples.
摘要:
A gasket having the form of a frame is provided with a curved portion at an inner or outer periphery thereof. When used in combination with a similarly configured gasket, the two gaskets may together, upon compression, form a pinch seal. The curved portion may be provided with a chemically resistant material.
摘要:
A core network entity includes a core network entity processing circuit. The core network entity processing circuit calculates a plurality of dormancy timer adjustment parameters in response to a received service request from a user equipment, and adjusts a dormancy timer for the user equipment based on (i) at least one load condition for the wireless network, and (ii) a comparison between each of the plurality of dormancy timer adjustment parameters and a corresponding one of a plurality of dormancy timer adjustment parameter threshold values. The core network entity then outputs the adjusted dormancy timer to a base station serving the user equipment.
摘要:
Systems and methods are disclosed for transmitting a text/multimedia message over a bearer interface of a mobile network. A first network element of the mobile network, such as an MSC for a Mobile Terminated (MT) text/multimedia message or a BSS for a Mobile Originated (MO) text/multimedia message, receives a text/multimedia message. The first network element communicates with a second network element of the mobile network using a signaling interface (e.g., an IOS A1 interface) to select a bearer connection on a non-voice circuit-mode bearer interface (e.g., an IOS A5 interface), and routes the text/multimedia message to the second network element over the selected connection on the non-voice circuit-mode bearer interface. The first network element may also select a bearer connection on a voice bearer interface (e.g., an IOS A2 interface) and route the text/multimedia message to the second network element over the selected bearer connection.
摘要:
Systems and methods are disclosed for transmitting a text/multimedia message over a bearer interface of a mobile network. A first network element of the mobile network, such as an MSC for a Mobile Terminated (MT) text/multimedia message or a BSS for a Mobile Originated (MO) text/multimedia message, receives a text/multimedia message. The first network element communicates with a second network element of the mobile network using a signaling interface (e.g., an IOS A1 interface) to select a bearer connection on a non-voice circuit-mode bearer interface (e.g., an IOS A5 interface), and routes the text/multimedia message to the second network element over the selected connection on the non-voice circuit-mode bearer interface. The first network element may also select a bearer connection on a voice bearer interface (e.g., an IOS A2 interface) and route the text/multimedia message to the second network element over the selected bearer connection.