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公开(公告)号：US20050268355A1
公开(公告)日：2005-12-01
申请号：US11169096
申请日：2005-06-28
申请人： Kenneth Gruys , Timothy Mitsky , Ganesh Kishore , Steven Slater , Stephen Padgette , David Stark
发明人： Kenneth Gruys , Timothy Mitsky , Ganesh Kishore , Steven Slater , Stephen Padgette , David Stark
CPC分类号： C12N9/0006 , C12N9/00 , C12N9/1029 , C12N9/88 , C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12P7/625
摘要： Nucleic acid sequences, vectors, recombinant host cells, transgenic plants, and methods for their preparation are disclosed. The nucleic acid sequences encode threonine deaminase proteins that catalyze the conversion of threonine to α-ketobutyrate. One or both of the amino acids at positions 447 and 481 of the encoded proteins can be selected from groups of particular amino acids. For example, the amino acid at position 447 can be alanine, isoleucine, valine, phenylalanine, tryptophan, or methionine. The amino acid at position 481 can be alanine, isoleucine, proline, phenylalanine, tryptophan, or methionine.
摘要翻译： 公开了核酸序列，载体，重组宿主细胞，转基因植物及其制备方法。 核酸序列编码苏氨酸脱氨酶蛋白，其催化苏氨酸转化为α-酮丁酸酯。 所编码的蛋白质的447位和481位氨基酸中的一个或两个可选自特定氨基酸的组。 例如，447位氨基酸可以是丙氨酸，异亮氨酸，缬氨酸，苯丙氨酸，色氨酸或甲硫氨酸。 位置481处的氨基酸可以是丙氨酸，异亮氨酸，脯氨酸，苯丙氨酸，色氨酸或甲硫氨酸。
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公开(公告)号：US20050289668A1
公开(公告)日：2005-12-29
申请号：US10840688
申请日：2004-05-06
申请人： Lisa Weaver , Timothy Mitsky , William Rapp , Kenneth Gruys , Jihong Liang , Gabriela Vaduva
发明人： Lisa Weaver , Timothy Mitsky , William Rapp , Kenneth Gruys , Jihong Liang , Gabriela Vaduva
CPC分类号： C12N9/88 , C12N15/8251
摘要： The present invention provides DNA constructs comprising exogenous polynucleotides encoding a threonine deaminase and/or AHAS. Transgenic plants transformed with the constructs, as well as seed and progeny dervied from these plants, are also provided. The transgenic plants have an increased level of one or more amino acids as compared to a non-transgenic plant of the same species.
摘要翻译： 本发明提供了包含编码苏氨酸脱氨酶和/或AHAS的外源多核苷酸的DNA构建体。 还提供了用这些植物转化的转基因植物，以及从这些植物衍生的种子和后代。 与相同物种的非转基因植物相比，转基因植物具有增加的一种或多种氨基酸水平。
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公开(公告)号：US07217865B2
公开(公告)日：2007-05-15
申请号：US10138927
申请日：2002-05-03
申请人： Lisa M Weaver , Jihong Liang , Ridong Chen , Soon Seog Jeong , Timothy Mitsky , Steven Slater , William Rapp
发明人： Lisa M Weaver , Jihong Liang , Ridong Chen , Soon Seog Jeong , Timothy Mitsky , Steven Slater , William Rapp
CPC分类号： C12N15/8254 , C12N9/88
摘要： The present invention provides transgenic plants transformed with an isolated DNA encoding a monomeric anthranilate synthase. The present invention also provides an isolated DNA encoding a monomeric anthranilate synthase from Agrobacterium tumefaciens. Transformation vectors and transformed plants containing the isolated DNA and seeds derived therefrom, are also provided.
摘要翻译： 本发明提供用编码单体邻氨基苯甲酸合酶的分离的DNA转化的转基因植物。 本发明还提供了编码来自根癌农杆菌的单体邻氨基苯甲酸合酶的分离的DNA。 还提供了转化载体和含有分离的DNA和从其衍生的种子的转化植物。
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公开(公告)号：US20050215505A1
公开(公告)日：2005-09-29
申请号：US11069543
申请日：2005-02-28
申请人： Soon Jeong , Ridong Chen , Timothy Mitsky
发明人： Soon Jeong , Ridong Chen , Timothy Mitsky
CPC分类号： C12N9/14 , A61K48/00 , C12Y306/01005
摘要： The present invention provides for design and therapeutic use of ADPase enhanced polypeptides, pharmaceutical compositions, and methods useful for preventing and reversing platelet aggregation and recruitment for the treatment and prevention of vascular disorders in mammals.
摘要翻译： 本发明提供了用于预防和逆转血小板聚集和募集用于治疗和预防哺乳动物血管疾病的ADP酶增强多肽，药物组合物和方法的设计和治疗应用。
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公开(公告)号：US06541259B1
公开(公告)日：2003-04-01
申请号：US09549848
申请日：2000-04-14
申请人： Michael W. Lassner , Beth Savidge , Timothy Mitsky , James Weiss , Martha Ann Post-Beittenmiller
发明人： Michael W. Lassner , Beth Savidge , Timothy Mitsky , James Weiss , Martha Ann Post-Beittenmiller
IPC分类号： C07H2104
CPC分类号： C12N9/0006 , A23D9/007 , A23K20/174 , C11B3/001 , C11B3/12 , C12N9/1085 , C12N9/88 , C12N15/8243 , C12N15/8247 , C12P17/06
摘要： Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.
摘要翻译： 提供核酸序列和方法用于产生具有改变的生育酚含量和组成的植物和种子。 该方法特别用于增加植物中的生育酚水平，以及在宿主植物细胞中提供期望的生育酚组合物。
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公开(公告)号：US20080086787A1
公开(公告)日：2008-04-10
申请号：US11773392
申请日：2007-07-03
申请人： Henry Valentin , Timothy Mitsky
发明人： Henry Valentin , Timothy Mitsky
CPC分类号： C12N9/001 , C07K14/47 , C12N9/90 , C12N15/8243
摘要： The present invention is in the field of plant genetics and biochemistry. More specifically, the invention relates to genes associated with the tocopherol biosynthesis pathway. The present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with the genes of the tocopherol biosynthesis pathway. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express proteins associated with the tocopherol pathway. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.
摘要翻译： 本发明在植物遗传学和生物化学领域。 更具体地，本发明涉及与生育酚生物合成途径相关的基因。 本发明提供并包括与生育酚生物合成途径的基因相关的核酸分子，蛋白质和抗体。 本发明还提供了利用这些试剂的方法，例如在基因分离，基因分析和转基因植物的生产中。 此外，本发明包括被修饰以表达与生育酚途径相关的蛋白质的转基因植物。 此外，本发明包括从生育酚生物合成途径生产产物的方法。
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公开(公告)号：US07141718B2
公开(公告)日：2006-11-28
申请号：US10437169
申请日：2003-05-13
CPC分类号： C12N9/0006 , A23D9/007 , A23K20/174 , C11B3/001 , C11B3/12 , C12N9/1085 , C12N9/88 , C12N15/8243 , C12N15/8247 , C12P17/06
摘要： Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.
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