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1.发明授权公开(公告)号：US09174222B2
公开(公告)日：2015-11-03
申请号：US13232781
申请日：2011-09-14
申请人： Lotien Richard Huang , Thomas Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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2.发明授权Devices and method for enrichment and alteration of cells and other particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US08585971B2
公开(公告)日：2013-11-19
申请号：US13452054
申请日：2012-04-20
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： G01N33/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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3.发明申请Devices And Method For Enrichment And Alteration Of Cells And Other Particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US20120225473A1
公开(公告)日：2012-09-06
申请号：US13452054
申请日：2012-04-20
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： C12M1/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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4.发明授权Devices and methods for enrichment and alteration of cells and other particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US08021614B2
公开(公告)日：2011-09-20
申请号：US11449149
申请日：2006-06-08
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： G01N33/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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5.发明申请DEVICES AND METHOD FOR ENRICHMENT AND ALTERATION OF CELLS AND OTHER PARTICLES 有权用于细胞和其他颗粒的增殖和改变的装置和方法公开(公告)号：US20120196273A1
公开(公告)日：2012-08-02
申请号：US13232781
申请日：2011-09-14
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： C12Q1/70 , G01N33/566 , G01N33/567 , C12Q1/02 , F03B11/00 , B01L99/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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6.发明申请Fractionation of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis 审中-公开使用不对称脉冲场电泳分离大分子公开(公告)号：US20090014332A1
公开(公告)日：2009-01-15
申请号：US12181827
申请日：2008-07-29
IPC分类号： B01D57/02
CPC分类号： C12N15/101 , Y10T436/10 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
摘要翻译： 提供了用于分离带电大分子如DNA的方法和装置。 将DNA溶液加载到包括障碍物阵列的矩阵中。 施加具有不同取向的两个不同场的交变电场。 交变电场是不对称的，因为一个场的持续时间或强度比另一个场强更强，否则是不对称的。 因此，DNA分子根据位点进行分级，并被驱动到基质的远侧，其中分离的DNA被回收。 分馏电场可用于负载和回收DNA以连续操作过程。
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7.发明申请METHOD FOR CONTINUOUS PARTICLE SEPARATION USING OBSTACLE ARRAYS ASYMMETRICALLY ALIGNED TO FIELDS 有权使用不对称地对齐的不正确的阵列的连续粒子分离方法公开(公告)号：US20120006728A1
公开(公告)日：2012-01-12
申请号：US13180823
申请日：2011-07-12
IPC分类号： B07B1/00
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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8.发明授权Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权使用与场不对称的障碍物阵列进行连续粒子分离的方法公开(公告)号：US07150812B2
公开(公告)日：2006-12-19
申请号：US10693091
申请日：2003-10-23
IPC分类号： G01N27/447 , G01N27/453 , G01N30/02 , G01N15/06
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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9.发明授权Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权使用与场不对称的障碍物阵列进行连续粒子分离的方法公开(公告)号：US08282799B2
公开(公告)日：2012-10-09
申请号：US13180823
申请日：2011-07-12
IPC分类号： G01N27/447 , G01N27/453 , G01N30/02 , G01N15/06
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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10.发明授权公开(公告)号：US06881317B2
公开(公告)日：2005-04-19
申请号：US10022189
申请日：2001-12-18
IPC分类号： C07H21/04 , B01L3/00 , C12N15/10 , G01N27/447 , C02F1/40 , C02F11/00 , C25B11/00 , G01N1/00 , G01N15/06
CPC分类号： C12N15/101 , Y10T436/10 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
摘要翻译： 提供了用于分离带电大分子如DNA的方法和装置。 将DNA溶液加载到包括障碍物阵列的矩阵中。 施加具有不同取向的两个不同场的交变电场。 交变电场是不对称的，因为一个场的持续时间或强度比另一个场强更强，否则是不对称的。 因此，DNA分子根据位点进行分级，并被驱动到基质的远侧，其中分离的DNA被回收。 分馏电场可用于负载和回收DNA以连续操作过程。
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