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    Paramyxoviruses comprising modified transcription start sequence
    4.
    发明授权
    Paramyxoviruses comprising modified transcription start sequence 失效
    副粘病毒包括修饰的转录起始序列

    公开(公告)号：US07144579B2

    公开(公告)日：2006-12-05

    申请号：US09979908

    申请日：2001-11-28

    申请人： Yoshiyuki Nagai ,  Atsushi Kato ,  Mamoru Hasegawa ,  Makoto Inoue

    发明人： Yoshiyuki Nagai ,  Atsushi Kato ,  Mamoru Hasegawa ,  Makoto Inoue

    IPC分类号： C07H19/00 ,  C12N15/63 ,  C12N15/66 ,  A61K39/12 ,  A61K39/155

    CPC分类号： C07K14/005 ,  A61K48/00 ,  C12N15/86 ,  C12N2760/18822 ,  C12N2760/18843

    摘要： The present invention provides virus vectors of the family Paramyxoviridae in which the transcription start (S) sequence has been modified so as to modify the expression of genes located downstream thereof, a method for producing the vectors, and uses thereof. By measuring the transcription initiation efficiency of the S sequence of each gene carried by Sendai viruses (SeV), it was clarified that the S sequence of F gene has a significantly lower ability to promote transcription than the other three S sequences. When the S sequence of the F gene of wild type Sendai virus was substituted by the S sequence of the P/M/HN gene-type showing a high transcription initiation efficiency, the F gene of the resultant Sendai virus mutant and genes located downstream thereof show elevated expression levels. It was also revealed that this mutant proliferates more quickly than the wild type. The vectors of this invention are useful in elevating the expression of foreign genes and producing pharmaceutical compositions and vaccines. Furthermore, by lowering virus gene expression from virus vectors, it is possible to suppress transcription and/or replication and reduce cytotoxicity of the vector genome.

    摘要翻译： 本发明提供了副粘病毒科的病毒载体，其中已经修饰了转录起始（S）序列，以便修饰其下游的基因的表达，生成载体的方法及其用途。 通过测量仙台病毒（SeV）携带的每个基因的S序列的转录起始效率，阐明了F基因的S序列比其他三个S序列具有显着较低的促进转录能力。 当野生型仙台病毒的F基因的S序列被显示高转录起始效率的P / M / HN基因型的S序列取代时，得到的仙台病毒突变体的F基因和位于其下游的基因 显示升高的表达水平。 还显示，该突变体比野生型增殖更快。 本发明的载体可用于提高外源基因的表达并产生药物组合物和疫苗。 此外，通过降低来自病毒载体的病毒基因表达，可以抑制转录和/或复制并降低载体基因组的细胞毒性。

    Method for production of dendritic cell
    6.
    发明授权
    Method for production of dendritic cell 有权
    树突状细胞的生产方法

    公开(公告)号：US08283163B2

    公开(公告)日：2012-10-09

    申请号：US12600103

    申请日：2008-05-12

    申请人： Makoto Inoue ,  Mamoru Hasegawa ,  Yoshikazu Yonemitsu ,  Yui Harada

    发明人： Makoto Inoue ,  Mamoru Hasegawa ,  Yoshikazu Yonemitsu ,  Yui Harada

    IPC分类号： C12N5/071 ,  C12N5/02

    CPC分类号： C12N5/0639 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26

    摘要： The present invention provides methods for producing DCs, which comprise the step of culturing DC precursor cells in the presence of multiple cytokines, dendritic cells produced thereby, and uses thereof. The methods of the present invention enable production of large quantities of DC precursors with a high ability to differentiate into DCs. The present invention enables one to obtain large quantities of DCs from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immunotherapy, treatment of infection, and such. Thus, an enhancement is expected for the effect of DC vaccines.

    摘要翻译： 本发明提供了生产DC的方法，其包括在多种细胞因子存在下培养DC前体细胞的步骤，由此产生的树突状细胞及其用途。 本发明的方法能够生产具有高分化成DC的能力的大量DC前体。 本发明能够从少量的DC前体细胞获得大量的DC，因此能够更容易地增加DC-基抗肿瘤免疫治疗中的DC给药次数，感染治疗等。 因此，预期DC疫苗的效果将得到改善。

    METHOD FOR PRODUCTION OF DENDRITIC CELL
    7.
    发明申请
    METHOD FOR PRODUCTION OF DENDRITIC CELL 有权
    生产细胞的方法

    公开(公告)号：US20100184214A1

    公开(公告)日：2010-07-22

    申请号：US12600103

    申请日：2008-05-12

    申请人： Makoto Inoue ,  Mamoru Hasegawa ,  Yoshikazu Yonemitsu ,  Yui Harada

    发明人： Makoto Inoue ,  Mamoru Hasegawa ,  Yoshikazu Yonemitsu ,  Yui Harada

    IPC分类号： C12N5/071 ,  C12N5/02

    CPC分类号： C12N5/0639 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26

    摘要： The present invention provides methods for producing DCs, which comprise the step of culturing DC precursor cells in the presence of multiple cytokines, dendritic cells produced thereby, and uses thereof. The methods of the present invention enable production of large quantities of DC precursors with a high ability to differentiate into DCs. The present invention enables one to obtain large quantities of DCs from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immunotherapy, treatment of infection, and such. Thus, an enhancement is expected for the effect of DC vaccines.

    摘要翻译： 本发明提供了生产DC的方法，其包括在多种细胞因子存在下培养DC前体细胞的步骤，由此产生的树突状细胞及其用途。 本发明的方法能够生产具有高分化成DC的能力的大量DC前体。 本发明能够从少量的DC前体细胞获得大量的DC，因此能够更容易地增加DC-基抗肿瘤免疫治疗中的DC给药次数，感染治疗等。 因此，预期DC疫苗的效果将得到改善。

    Envelope gene-deficient Paramyxovirus vector
    9.
    发明授权
    Envelope gene-deficient Paramyxovirus vector 有权
    包膜基因缺陷型副粘病毒载体

    公开(公告)号：US07226786B2

    公开(公告)日：2007-06-05

    申请号：US10316535

    申请日：2002-12-10

    申请人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Fumino Tokito ,  Takahiro Hirata ,  Tsuyoshi Tokusumi ,  Makoto Inoue ,  Yumiko Tokusumi

    发明人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Fumino Tokito ,  Takahiro Hirata ,  Tsuyoshi Tokusumi ,  Makoto Inoue ,  Yumiko Tokusumi

    IPC分类号： C12N15/00 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2760/18823 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2760/18861 ,  C12N2800/30 ,  C12N2810/6081

    摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.

    摘要翻译： 通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。 此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。 此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。 此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。 通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。 此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。 从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。 此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。 用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。