公开(公告)号：US20100183630A1

公开(公告)日：2010-07-22

申请号：US11922881

申请日：2006-06-29

申请人： Marcelo Filgueira ,  Daniel Chelsky ,  Joel Lanoix ,  Kevin Eng ,  Pierre Thibault ,  Denis Faubert ,  Navdeep Jaitly ,  Sylvain Brunet ,  Frank R.M. Aguilera ,  John S.-C. Tsang ,  Michael Hu ,  Joachim B. Ostermann ,  Marguerite Boulos ,  Paul E. Kearney ,  Heather Butler ,  Sajani Swamy ,  Lyes Hamaidi

发明人： Marcelo Filgueira ,  Daniel Chelsky ,  Joel Lanoix ,  Kevin Eng ,  Pierre Thibault ,  Denis Faubert ,  Navdeep Jaitly ,  Sylvain Brunet ,  Frank R.M. Aguilera ,  John S.-C. Tsang ,  Michael Hu ,  Joachim B. Ostermann ,  Marguerite Boulos ,  Paul E. Kearney ,  Heather Butler ,  Sajani Swamy ,  Lyes Hamaidi

IPC分类号： C07K16/30 ,  C07K16/46 ,  A61K39/395 ,  G01N33/574 ,  A61P35/04

CPC分类号： G01N33/57419 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/4748 ,  G01N2500/04

摘要： Surprisingly, the present inventors have discovered that expression of TAT-005 protein in human patients is associated with cancer, and that the over-expressed protein is present in plasma membrane fractions. Thus, the present inventors have discovered that TAT-005 is associated with abnormal development and growth, and may be useful as a target for the identification of anti-cancer com\ pounds, including antibodies for use in immunotherapy. Accordingly, the present invention provides methods for the identification of compounds that inhibit TAT-005 expression or activity, comprising: contacting a candidate compound with a TAT-005 and detecting the presence or absence of binding between said compound and said TAT-005, or detecting a change in TAT-005 expression or activity. Methods are also included for the identification of compounds that modulate TAT-005 expression or activity, comprising: administering a compound to a cell or cell population, and detecting a change in TAT-005 expression or activity. The methods of the invention are useful for the identification of anti-cancer compounds.

摘要翻译： 令人惊奇的是，本发明人已经发现，人类患者中TAT-005蛋白的表达与癌症相关，并且过度表达的蛋白质存在于质膜组分中。 因此，本发明人已经发现，TAT-005与异常发育和生长相关，并且可用作鉴定抗癌药物的靶标，包括用于免疫治疗的抗体。 因此，本发明提供了用于鉴定抑制TAT-005表达或活性的化合物的方法，包括：使候选化合物与TAT-005接触并检测所述化合物与所述TAT-005之间是否存在结合，或 检测TAT-005表达或活性的变化。 还包括用于鉴定调节TAT-005表达或活性的化合物的方法，包括：向细胞或细胞群体施用化合物，并检测TAT-005表达或活性的变化。 本发明的方法可用于鉴定抗癌化合物。

公开(公告)号：US20070106065A1

公开(公告)日：2007-05-10

申请号：US11344933

申请日：2006-01-31

申请人： Paul Kearney ,  Heather Butler ,  Navdeep Jaitly ,  Sajani Swamy ,  Lyes Hamaidi ,  Marcelo Filgueira ,  Pierre Thibault ,  Denis Faubert ,  Daniel Chelsky ,  Joel Lanoix ,  Sylvain Brunet ,  Kevin Eng ,  Frank Aguilera ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Marguerite Boulos

发明人： Paul Kearney ,  Heather Butler ,  Navdeep Jaitly ,  Sajani Swamy ,  Lyes Hamaidi ,  Marcelo Filgueira ,  Pierre Thibault ,  Denis Faubert ,  Daniel Chelsky ,  Joel Lanoix ,  Sylvain Brunet ,  Kevin Eng ,  Frank Aguilera ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Marguerite Boulos

IPC分类号： C07K14/82 ,  C07K16/30 ,  C12P21/06 ,  A61K39/395 ,  A61K39/00 ,  C12P21/08 ,  C12P21/04

CPC分类号： C07K14/82 ,  C07K14/4748 ,  C07K16/3046

摘要： Surprisingly, the present inventors have discovered that expression of TAT-001 protein in human patients is associated with cancer, and that the overexpressed protein is present in plasma membrane fractions. Thus, the present inventors have discovered that TAT-001 is associated with abnormal development and growth, and may be useful as a target for the identification of anti-cancer compounds, including antibodies for use in immunotherapy. Accordingly, the present invention provides methods for the identification of compounds that inhibit TAT-001 expression or activity, comprising: contacting a candidate compound with a TAT-001 and detecting the presence or absence of binding between said compound and said TAT-001, or detecting a change in TAT-001 expression or activity. Methods are also included for the identification of anti-cancer compounds and compounds that modulate TAT-001 expression or activity, comprising: administering a compound to a cell or cell population, and detecting a change in TAT-001 expression or activity.

摘要翻译： 令人惊奇的是，本发明人已经发现，人类患者中TAT-001蛋白的表达与癌症有关，并且过表达的蛋白质存在于质膜组分中。 因此，本发明人已经发现，TAT-001与异常发育和生长有关，可用作鉴定抗癌化合物的靶标，包括用于免疫治疗的抗体。 因此，本发明提供了用于鉴定抑制TAT-001表达或活性的化合物的方法，包括：使候选化合物与TAT-001接触并检测所述化合物与所述TAT-001之间是否存在结合，或 检测TAT-001表达或活性的变化。 还包括用于鉴定调节TAT-001表达或活性的抗癌化合物和化合物的方法，包括：向细胞或细胞群施用化合物，并检测TAT-001表达或活性的变化。

公开(公告)号：US20060269944A1

公开(公告)日：2006-11-30

申请号：US11429113

申请日：2006-05-04

申请人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

发明人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

IPC分类号： C12Q1/68 ,  G06F19/00 ,  G01N33/48

CPC分类号： G01N33/6848 ,  Y10T436/24

摘要： The present invention is directed to computer automated methods and systems for identifying and characterizing biomolecules in a biological sample. Mass spectrometry measurements are obtained on biomolecules in a sample. These measurements are analyzed to determine the abundance of the biomolecules in the sample, and the abundance measurements are coupled with one or more distinguishing characteristics of biomolecules they are associated with, thereby permitting computer-mediated comparison of abundances of biomolecules from multiple biological samples.

摘要翻译： 本发明涉及用于识别和表征生物样品中生物分子的计算机自动化方法和系统。 在样品中的生物分子上获得质谱测量。 分析这些测量以确定样品中生物分子的丰度，并且丰度测量与其相关联的生物分子的一个或多个区别特征相结合，从而允许计算机介导的来自多个生物样品的生物分子丰度的比较。

公开(公告)号：US20060003385A1

公开(公告)日：2006-01-05

申请号：US11188954

申请日：2005-07-25

申请人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

发明人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00

CPC分类号： G01N33/6848 ,  Y10T436/24

摘要： The present invention is directed to computer automated methods and systems for identifying and characterizing biomolecules in a biological sample. Mass spectrometry measurements are obtained on biomolecules in a sample. These measurements are analyzed to determine the abundance of the biomolecules in the sample, and the abundance measurements are coupled with one or more distinguishing characteristics of biomolecules they are associated with, thereby permitting computer-mediated comparison of abundances of biomolecules from multiple biological samples.

公开(公告)号：US20060031023A1

公开(公告)日：2006-02-09

申请号：US11246325

申请日：2005-10-06

申请人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

发明人： Frank Aguilera ,  Denis Faubert ,  Marguerite Boulos ,  John Tsang ,  Michael Hu ,  Joachim Ostermann ,  Paul Kearney ,  Pierre Thibault

IPC分类号： G06F19/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N33/6848 ,  Y10T436/24

摘要： The present invention is directed to computer automated methods and systems for identifying and characterizing biomolecules in a biological sample. Mass spectrometry measurements are obtained on biomolecules in a sample. These measurements are analyzed to determine the abundance of the biomolecules in the sample, and the abundance measurements are coupled with one or more distinguishing characteristics of biomolecules they are associated with, thereby permitting computer-mediated comparison of abundances of biomolecules from multiple biological samples.

公开(公告)号：US08393209B2

公开(公告)日：2013-03-12

申请号：US12936153

申请日：2009-03-12

申请人： Pierre Thibault

发明人： Pierre Thibault

IPC分类号： G01F23/26 ,  G08B21/00

CPC分类号： G01F23/268

摘要： Capacitive detector and measuring device integrating it; the detector including: a ribbon made of a dielectric material, first and second electrodes in the form of combs formed on a first surface of said ribbon and a third electrode formed on the other surface of said ribbon opposite said first and second electrodes; the thickness (e) of the dielectric ribbon is less than or equal to said determined period (λ) divided by four times PI, i.e.: λ/4π≧e; and the ratio resulting from the division, as numerator, of the capacitance (C) between two adjacent branches of said first and second electrodes in the presence of at least one fluid with a dielectric permittivity (∈f) and, as denominator, by the dielectric permittivity (∈f) of the fluid multiplied by the capacitance (Co) between the two adjacent branches of the first and second electrodes in the presence of the vacuum is greater than or equal to one, i.e.: C/(∈f*Co)≧1. Method for manufacturing a detector fulfilling the above conditions.

摘要翻译： 整合电容检测器和测量装置; 所述检测器包括：由介电材料制成的带，形成在所述带的第一表面上的梳形形式的第一和第二电极以及形成在所述带的与所述第一和第二电极相对的另一表面上的第三电极; 电介质带的厚度（e）小于或等于所述确定的周期（λ）除以PI的四倍，即：λ/ 4＆pgr;≥e; 以及在存在具有介电常数（εf）的至少一种流体的情况下，作为分子除以所述第一和第二电极的两个相邻分支之间的电容（C）所产生的比率，并且作为分母，由 在存在真空的情况下，流体的介电常数（εf）乘以第一和第二电极的两个相邻分支之间的电容（Co）大于或等于1，即：C /（∈f* Co ）≧1。 制造满足上述条件的检测器的方法。

公开(公告)号：US20120276529A1

公开(公告)日：2012-11-01

申请号：US13349459

申请日：2012-01-12

申请人： Frédéric Galisson ,  Louiza Mahrouche ,  Eric Bonneil ,  Mounira Chelbi-Alix ,  Sylvain Meloche ,  Pierre Thibault

发明人： Frédéric Galisson ,  Louiza Mahrouche ,  Eric Bonneil ,  Mounira Chelbi-Alix ,  Sylvain Meloche ,  Pierre Thibault

IPC分类号： G01N33/566 ,  C07K16/18 ,  C12Q1/68 ,  G01N21/64 ,  G01N27/62

CPC分类号： G01N33/6848 ,  C07K14/47 ,  C07K16/18 ,  C12Q1/25 ,  C12Q1/37 ,  G01N33/5008 ,  G01N33/6842 ,  G01N2333/9015 ,  G01N2440/00

摘要： Disclosed herein are substantially pure nucleic acids encoding mutated SUMO isoforms, polypeptides, vectors, cells and methods of their use to identify and quantify protein SUMOylation in mammalian cells. Also disclosed is a dual affinity method for detecting a mutated SUMOylated protein substrate fragment.

摘要翻译： 本文公开的是编码突变的SUMO同种型，多肽，载体，细胞及其用于鉴定和定量哺乳动物细胞中的蛋白质SUMO化的方法的基本上纯的核酸。 还公开了用于检测突变的SUMO化蛋白质底物片段的双重亲和力方法。

公开(公告)号：US06605472B1

公开(公告)日：2003-08-12

申请号：US09169146

申请日：1998-10-09

申请人： Cameron Skinner ,  Thompson Tang ,  D. Jed Harrison ,  Nicolas Bings ,  Can Wang ,  Gregor Ocvirk ,  Jianjun Li ,  Pierre Thibault

发明人： Cameron Skinner ,  Thompson Tang ,  D. Jed Harrison ,  Nicolas Bings ,  Can Wang ,  Gregor Ocvirk ,  Jianjun Li ,  Pierre Thibault

IPC分类号： G01N3300

CPC分类号： B01L3/502707 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0421 ,  G01N27/44791 ,  G01N30/6034 ,  G01N30/6095 ,  G01N30/7266 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A method is provided for joining a microchip device to a capillary tube. The microchip device has a capillary channel opening onto an edge surface of the device. A short hole is drilled into the edge surface, aligned with the capillary channel. The drilling is done with a flat bottom, preferably by a two-step drilling process. Then, the end of the capillary can be inserted into the hole so that its end is substantially flush with the flat bottom of the hole, thereby eliminating dead volume. Testing has shown that this connection provides very little band broadening of samples transported through the capillary channel into the capillary tube. The tip of the capillary tube can be tapered, so that it is suitable for use as an electrospray source for a mass spectrometer.

摘要翻译： 提供了一种将微芯片器件连接到毛细管上的方法。 微芯片装置具有在装置的边缘表面上开口的毛细通道。 在边缘表面钻出一个短孔，与毛细管通道对齐。 钻孔采用平坦的底部，优选通过两步钻孔工艺进行。 然后，毛细管的端部可以插入孔中，使得其端部与孔的平坦底部基本齐平，从而消除死体积。 测试表明，这种连接提供了通过毛细管通道进入毛细管的样品的极小带宽扩大。 毛细管的尖端可以是锥形的，使得其适合用作质谱仪的电喷雾源。

公开(公告)号：US09046527B2

公开(公告)日：2015-06-02

申请号：US13349459

申请日：2012-01-12

申请人： Frédéric Galisson ,  Louiza Mahrouche ,  Eric Bonneil ,  Mounira Chelbi-Alix ,  Sylvain Meloche ,  Pierre Thibault

发明人： Frédéric Galisson ,  Louiza Mahrouche ,  Eric Bonneil ,  Mounira Chelbi-Alix ,  Sylvain Meloche ,  Pierre Thibault

IPC分类号： C07K16/18 ,  C07K1/22 ,  C12P21/08 ,  C07K5/103 ,  G01N33/53 ,  G01N30/72 ,  G01N33/68 ,  C07K14/47 ,  C12Q1/25 ,  C12Q1/37 ,  G01N33/50

CPC分类号： G01N33/6848 ,  C07K14/47 ,  C07K16/18 ,  C12Q1/25 ,  C12Q1/37 ,  G01N33/5008 ,  G01N33/6842 ,  G01N2333/9015 ,  G01N2440/00

摘要： Disclosed herein are substantially pure nucleic acids encoding mutated SUMO isoforms, polypeptides, vectors, cells and methods of their use to identify and quantify protein SUMOylation in mammalian cells. Also disclosed is a dual affinity method for detecting a mutated SUMOylated protein substrate fragment.

摘要翻译： 本文公开的是编码突变的SUMO同种型，多肽，载体，细胞及其用于鉴定和定量哺乳动物细胞中的蛋白质SUMO化的方法的基本上纯的核酸。 还公开了用于检测突变的SUMO化蛋白质底物片段的双重亲和力方法。

公开(公告)号：US5587791A

公开(公告)日：1996-12-24

申请号：US313690

申请日：1994-09-27

申请人： Claude Belleville ,  Richard Van Neste ,  Serge Caron ,  Alain C. Houle ,  Pierre Thibault

发明人： Claude Belleville ,  Richard Van Neste ,  Serge Caron ,  Alain C. Houle ,  Pierre Thibault

IPC分类号： G01R15/24 ,  G01B9/02 ,  G02B6/00

CPC分类号： G01R15/246

摘要： The interferometric current sensor is for measuring an electrical current. It comprises a light source for generating a light beam; a single mode birefringent waveguide having a linear birefringence and a circular birefringence, the circular and linear birefringences having respectively given orders of magnitude, the order of magnitude of the circular birefringence being equal or higher than the order of magnitude of the linear birefringence, the waveguide having a first loop portion for encircling the electrical current; a beamsplitter having a first input port optically coupled to the light source, and second and third input/output ports optically coupled to ends of the waveguide for launching counter-propagating light beams into the respective ends of the waveguide and for receiving the counter-propagating light beams therefrom; and a pseudo-depolarizer optically coupled in series with the waveguide for converting each of the counter-propagating light beams into a predetermined ratio of useful counter-propagating light signals and useless counter-propagating light signals. It also comprises an optical detector optically coupled to the ends of the waveguide via the beamsplitter for detecting a light intensity, the light intensity is representative of the current.

摘要翻译： 干涉电流传感器用于测量电流。 它包括用于产生光束的光源; 具有线性双折射和圆形双折射的单模双折射波导，圆形和线性双折射分别具有数量级，圆形双折射的数量级等于或高于线性双折射的数量级，波导 具有用于环绕电流的第一回路部分; 具有光耦合到光源的第一输入端口的分束器和光学耦合到波导的端部的第二和第三输入/输出端口，用于将反向传播的光束发射到波导的相应端部并且用于接收反向传播的 光束; 以及与波导串联光学耦合的伪去偏振器，用于将每个反向传播的光束转换成有效的反向传播光信号和无用的反向传播光信号的预定比例。 它还包括光学检测器，其通过分光器光学耦合到波导的端部，用于检测光强度，光强度代表电流。