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1.发明授权Hemoglobin mutants with increased soluble expression and/or reduced nitric oxide scavenging 失效具有增加的可溶性表达和/或减少的一氧化氮清除的血红蛋白突变体公开(公告)号：US06455676B1
公开(公告)日：2002-09-24
申请号：US09403208
申请日：2000-04-25
申请人： Michael J. Weickert , Christopher B. Glascock , Antony J. Mathews , Douglas D. Lemon , Daniel H. Doherty , John S. Olson
发明人： Michael J. Weickert , Christopher B. Glascock , Antony J. Mathews , Douglas D. Lemon , Daniel H. Doherty , John S. Olson
IPC分类号： C07K14805
CPC分类号： C07K14/805 , A61K38/00
摘要： The invention relates to novel recombinant hemoglobins having reduced nitric oxide scavenging and/or increased high soluble expression. The invention further relates to methods of increasing the soluble expression of recombinant hemoglobin by adding exogenous hemin in molar excess of the heme binding sites of recombinant hemoglobin.
摘要翻译： 本发明涉及具有减少的一氧化氮清除和/或增加的高可溶性表达的新型重组血红蛋白。 本发明还涉及通过在重组血红蛋白的血红素结合位点的摩尔过量中加入外源血红素来增加重组血红蛋白的可溶性表达的方法。
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公开(公告)号：US08309796B2
公开(公告)日：2012-11-13
申请号：US13170011
申请日：2011-06-27
申请人： Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
发明人： Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
CPC分类号： C12P7/6427 , C12N9/93 , C12N15/52 , C12N15/8247 , C12N15/8273 , C12P7/62 , C12P7/625 , C12P7/6472
摘要： Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.
摘要翻译： 公开了嵌合多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。
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公开(公告)号：US20120064573A1
公开(公告)日：2012-03-15
申请号：US13088830
申请日：2011-04-18
IPC分类号： C12P21/00
CPC分类号： C12P21/02 , A61K38/27 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/56 , C07K14/61 , C07K14/72 , C12P21/005
摘要： The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.
摘要翻译： 本发明涉及制备具有游离半胱氨酸的可溶性蛋白质的新方法，其中宿主细胞暴露于半胱氨酸阻断剂。 然后可以对通过该方法产生的可溶性蛋白质进行修饰以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化的蛋白质。
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4.发明申请公开(公告)号：US20090269804A1
公开(公告)日：2009-10-29
申请号：US12001639
申请日：2007-12-11
CPC分类号： C07K14/52 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/515 , C07K14/535 , C07K14/56 , C07K14/61 , C07K14/78
摘要： The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
摘要翻译： 本发明涉及制备和重折叠具有游离半胱氨酸的不溶性或聚集蛋白质的新方法，其中表达蛋白质的宿主细胞暴露于半胱氨酸阻断剂。 然后可以修饰通过新方法产生的可溶的，重折叠的蛋白质以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化蛋白质。
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公开(公告)号：US07153943B2
公开(公告)日：2006-12-26
申请号：US10298148
申请日：2002-11-15
IPC分类号： C07K14/435 , C07K14/52 , C07K14/53 , C07K14/535 , A61K38/18 , A61K38/22 , A61K45/00 , C12N15/09 , C12N15/16
CPC分类号： A61K38/193 , A61K47/60 , C07K14/475 , C07K14/505 , C07K14/52 , C07K14/535 , C07K14/5431 , C07K14/61
摘要： The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby. Also disclosed are therapeutic methods for using the cysteine variants of the invention.
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6.发明授权Method for preparing recombinant granulocyte colony stimulating factor cysteine muteins 有权重组粒细胞集落刺激因子半胱氨酸突变蛋白的制备方法公开(公告)号：US08932828B2
公开(公告)日：2015-01-13
申请号：US12893764
申请日：2010-09-29
IPC分类号： C12P21/00 , C07K1/00 , C07K1/14 , C07K1/16 , C07K2/00 , C07K14/515 , C07K1/113 , C07K14/505 , C07K14/56 , C07K14/535 , C07K14/52 , C07K14/61 , A61K47/48 , C07K14/78
CPC分类号： C07K14/52 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/515 , C07K14/535 , C07K14/56 , C07K14/61 , C07K14/78
摘要： The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
摘要翻译： 本发明涉及制备和重折叠具有游离半胱氨酸的不溶性或聚集蛋白质的新方法，其中表达蛋白质的宿主细胞暴露于半胱氨酸阻断剂。 然后可以修饰通过新方法产生的可溶的，重折叠的蛋白质以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化蛋白质。
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7.发明申请DERIVATIVES OF GROWTH HORMONE AND RELATED PROTEINS, AND METHODS OF USE THEREOF 有权生长激素和相关蛋白的衍生物及其使用方法公开(公告)号：US20110172151A1
公开(公告)日：2011-07-14
申请号：US12886876
申请日：2010-09-21
IPC分类号： A61K38/22 , C07K14/535 , A61P37/02
CPC分类号： A61K38/193 , A61K47/60 , C07K14/475 , C07K14/505 , C07K14/52 , C07K14/535 , C07K14/5431 , C07K14/61
摘要： The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby. Also disclosed are therapeutic methods for using the cysteine variants of the invention.
摘要翻译： 生长激素超基因家族包含大于20个结构相关的细胞因子和生长因子。 提供了一种用于产生这些蛋白质的位点特异性，生物活性缀合物的通用方法。 该方法包括将半胱氨酸残基添加到蛋白质的非必需区域中，或者使用定点突变将蛋白质中的非必需氨基酸取代半胱氨酸残基，然后将半胱氨酸反应性聚合物或其它类型的半胱氨酸反应性部分共价偶联到 通过添加的半胱氨酸残基的蛋白质。 本文公开了用于添加半胱氨酸残基或将半胱氨酸取代引入蛋白质的优选位点，以及由此产生的蛋白质和蛋白质衍生物。 还公开了使用本发明的半胱氨酸变体的治疗方法。
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公开(公告)号：US5514791A
公开(公告)日：1996-05-07
申请号：US232416
申请日：1994-04-25
申请人： Daniel H. Doherty , Donna M. Ferber , John D. Marrelli , Rebecca W. Vanderslice , Randal A. Hassler
发明人： Daniel H. Doherty , Donna M. Ferber , John D. Marrelli , Rebecca W. Vanderslice , Randal A. Hassler
CPC分类号： C12R1/64 , C08B37/0033 , C09K8/08 , C12N15/00 , C12N15/01 , C12P19/06 , Y10S435/822
摘要： Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta- 1,4! configuration. The inner D-mannose moieties are linked in an alpha- 1,3! configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta- 1,2! configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta- 1,4! configuration. Processes for preparing the polysaccharide polymers are also provided.
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公开(公告)号：US08697398B2
公开(公告)日：2014-04-15
申请号：US13038326
申请日：2011-03-01
CPC分类号： C12N15/63 , C07K14/245 , C07K16/1232 , C08B37/0069 , C08L5/08 , C12N15/52 , C12N15/70 , C12N15/74 , C12P19/04 , C12P19/26
摘要： The invention relates to the field of recombinant DNA technology for the production of chondroitin, including the production of chondroitin sulfate via a combination of recombinant bacterial fermentation and post-fermentation sulfation.
摘要翻译： 本发明涉及用于生产软骨素的重组DNA技术领域，包括通过重组细菌发酵和后发酵硫酸化的组合生产硫酸软骨素。
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公开(公告)号：US20120021470A1
公开(公告)日：2012-01-26
申请号：US13170011
申请日：2011-06-27
申请人： Craig A. WEAVER , Ross Zirkle , Daniel H. Doherty , James G. Metz
发明人： Craig A. WEAVER , Ross Zirkle , Daniel H. Doherty , James G. Metz
CPC分类号： C12P7/6427 , C12N9/93 , C12N15/52 , C12N15/8247 , C12N15/8273 , C12P7/62 , C12P7/625 , C12P7/6472
摘要： Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.
摘要翻译： 公开了嵌合多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。
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