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    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION
    5.
    发明申请
    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION 有权
    二次探针和夹钳组合物和方法进行目标杂交检测

    公开(公告)号：US20100291557A1

    公开(公告)日：2010-11-18

    申请号：US12538823

    申请日：2009-08-10

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Binary probe and clamp composition
    6.
    发明授权
    Binary probe and clamp composition 有权
    二进制探针和夹具组成

    公开(公告)号：US07057025B2

    公开(公告)日：2006-06-06

    申请号：US10112677

    申请日：2002-03-28

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Binary probe and clamp composition and methods for a target hybridization detection
    7.
    发明授权
    Binary probe and clamp composition and methods for a target hybridization detection 有权
    用于靶杂交检测的二进制探针和夹钳组成及方法

    公开(公告)号：US06432642B1

    公开(公告)日：2002-08-13

    申请号：US09232000

    申请日：1999-01-15

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Automated polypeptide synthesis process
    8.
    发明授权
    Automated polypeptide synthesis process 失效
    自动多肽合成过程

    公开(公告)号：US4816513A

    公开(公告)日：1989-03-28

    申请号：US53324

    申请日：1987-05-22

    申请人： John Bridgham ,  Timothy G. Geiser ,  Michael W. Hunkapiller ,  Stephen B. H. Kent ,  Mark P. Marriott ,  Paul O. Ramstad ,  Eric S. Nordman

    发明人： John Bridgham ,  Timothy G. Geiser ,  Michael W. Hunkapiller ,  Stephen B. H. Kent ,  Mark P. Marriott ,  Paul O. Ramstad ,  Eric S. Nordman

    IPC分类号： C07K1/00 ,  B01J19/00 ,  C07K1/04 ,  C40B40/10 ,  C40B60/14 ,  C08L89/00 ,  C07C103/52

    CPC分类号： C07K1/045 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00389 ,  B01J2219/00484 ,  B01J2219/00488 ,  B01J2219/0049 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00725 ,  C40B40/10 ,  C40B60/14

    摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in the production of a peptide for any given selection of cycles.

    摘要翻译： 提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。 该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。 还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。 还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。 活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。 还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统 在肽序列中。 还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。 控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。 此外，已经开发了一种算法，其提供用于任何给定选择周期的肽的生产的最佳效率。

    Restriction enzyme mediated adapter
    10.
    发明授权
    Restriction enzyme mediated adapter 有权
    限制酶介导的适配器

    公开(公告)号：US06258539B1

    公开(公告)日：2001-07-10

    申请号：US09303774

    申请日：1999-04-30

    申请人： Michael W. Hunkapiller ,  John H. Richards

    发明人： Michael W. Hunkapiller ,  John H. Richards

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2525/191 ,  C12Q2521/313 ,  C12Q2525/179 ,  C12Q2525/161

    摘要： The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a polynucleotide composition comprising multiple diverse polynucleotide sequences. The subject methods and compositions may also be applied to analyze or identify single polynucleotides; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides, e.g., cDNA libraries. Most embodiments of the invention involve hybridizing terminus probes (of known base sequence) to adapter-modified restriction fragment generated from polynucleotide for analysis, and subsequently joining the terminus probes and internal fragment probes to each other. The terminus probe hybridizes to bases of restriction endonuclease recognition site present at the terminus of a restriction fragment generated from the polynucleotide for analysis. The terminus probes and internal fragment probes may be marked so as to facilitate the simultaneous testing of multiple polynucleotides for the presence of many possible nucleotide base sequences. The identity or expression of a particular polynucleotide of interest may be ascertained (or at least partially determined) by producing a short identifier sequence derived from the nucleotide base sequence information obtained from (1) the hybridization of a terminus probe, and (2) the recognition site of a restriction endonuclease used to generate the polynucleotide molecule of interest. Multiple identification sequences may be obtained in parallel, thereby permitting the rapid characterization of a large number of diverse polynucleotides. Parallel processing may be achieved by differentially marking terminus probes or internal fragment probes. Parallel processing may be achieved by using ordered arrays of oligonucleotides that are terminus probes.

    摘要翻译： 本发明涉及用于同时分析包含多种不同多核苷酸序列的多核苷酸组合物的多种不同多核苷酸的方法和组合物。 主题方法和组合物也可以用于分析或鉴定单个多核苷酸; 然而，本发明的方法和组合物特别可用于分析大量不同种群的多核苷酸，例如cDNA文库。 本发明的大多数实施方案涉及将已知碱基序列的末端探针与由多核苷酸产生的衔接子修饰的限制性片段杂交用于分析，然后将末端探针和内部片段探针彼此连接。 末端探针与存在于由多核苷酸产生的限制性片段的末端存在的限制性内切核酸酶识别位点的碱基杂交，用于分析。 可以标记末端探针和内部片段探针，以促进多个多核苷酸的同时测试以存在许多可能的核苷酸碱基序列。 通过产生源自从（1）末端探针的杂交获得的核苷酸碱基序列信息的短标识符序列，可以确定（或至少部分地确定）特定感兴趣的多核苷酸的身份或表达，和（2） 用于产生感兴趣的多核苷酸分子的限制性内切核酸酶的识别位点。 可以并行获得多个鉴定序列，从而允许快速表征大量不同多核苷酸。 通过差异标记末端探针或内部片段探针可以实现并行处理。 可以通过使用作为末端探针的寡核苷酸的有序阵列来实现并行处理。