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    Quantitative assay for detection of newly synthesized RNA in a cell-free system and identification of RNA synthesis inhibitors
    1.
    发明授权
    Quantitative assay for detection of newly synthesized RNA in a cell-free system and identification of RNA synthesis inhibitors 失效
    用于在无细胞系统中检测新合成的RNA的定量测定和RNA合成抑制剂的鉴定

    公开(公告)号：US07985541B2

    公开(公告)日：2011-07-26

    申请号：US10593683

    申请日：2005-03-23

    申请人： Mingjun Huang ,  Yongnian Sun ,  Wengang Yang

    发明人： Mingjun Huang ,  Yongnian Sun ,  Wengang Yang

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/707 ,  C12N2770/24011 ,  C12N2770/24211 ,  C12Q1/6816

    摘要： Methods for detecting populations of newly synthesized and newly initiated RNAs in a cell-free system are described. Suitable templates for the populations of newly synthesized and newly initiated RNAs are viral replicon RNAs, particularly HCV viral replicon RNA. The newly synthesized and newly initiated RNAs may be formed in the presence of a labeled nucleotide analog suitable for detection and/or quantitation of the RNAs. The assay may be employed to identify small molecule inhibitors of RNA synthesis of positive strand RNA viruses such as Hepatitis C Virus.

    摘要翻译： 描述了在无细胞系统中检测新合成和新引发的RNA的群体的方法。 用于新合成和新引发的RNA的群体的合适模板是病毒复制子RNA，特别是HCV病毒复制子RNA。 新合成的和新引发的RNA可以在适于检测和/或定量RNA的标记的核苷酸类似物的存在下形成。 该测定可以用于鉴定正链RNA病毒如丙型肝炎病毒的RNA合成的小分子抑制剂。

    Quantitative assay for detection of newly synthesized rna in a cell-free system and indentification of rna synthesis inhibitors
    2.
    发明申请
    Quantitative assay for detection of newly synthesized rna in a cell-free system and indentification of rna synthesis inhibitors 失效
    用于检测无细胞系统中新合成的rna的定量测定和rna合成抑制剂的鉴定

    公开(公告)号：US20070141558A1

    公开(公告)日：2007-06-21

    申请号：US10593683

    申请日：2005-03-23

    申请人： Mingjun Huang ,  Yongnian Sun ,  Wengan Yang

    发明人： Mingjun Huang ,  Yongnian Sun ,  Wengan Yang

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C12Q1/707 ,  C12N2770/24011 ,  C12N2770/24211 ,  C12Q1/6816

    摘要： Methods for detecting populations of newly synthesized and newly initiated RNAs in a cell-free system are described. Suitable templates for the populations of newly synthesized and newly initiated RNAs are viral replicon RNAs, particularly HCV viral replicon RNA. The newly synthesized and newly initiated RNAs may be formed in the presence of a labeled nucleotide analog suitable for detection and/or quantitation of the RNAs. The assay may be employed to identify small molecule inhibitors of RNA synthesis of positive strand RNA viruses such as Hepatitis C Virus.

    摘要翻译： 描述了在无细胞系统中检测新合成和新引发的RNA的群体的方法。 用于新合成和新引发的RNA的群体的合适模板是病毒复制子RNA，特别是HCV病毒复制子RNA。 新合成的和新引发的RNA可以在适于检测和/或定量RNA的标记的核苷酸类似物的存在下形成。 该测定可以用于鉴定正链RNA病毒如丙型肝炎病毒的RNA合成的小分子抑制剂。

    CELLS EXPRESSING CHIMERIC PROTEINS AND ASSAYS USING SUCH CELLS
    4.
    发明申请
    CELLS EXPRESSING CHIMERIC PROTEINS AND ASSAYS USING SUCH CELLS 审中-公开
    细胞表达CHIMERIC蛋白和使用这种细胞的测定

    公开(公告)号：US20100093018A1

    公开(公告)日：2010-04-15

    申请号：US12597661

    申请日：2008-04-28

    申请人： Xiaohong Hou ,  Mingjun Huang ,  Wengang Yang

    发明人： Xiaohong Hou ,  Mingjun Huang ,  Wengang Yang

    IPC分类号： C12Q1/02 ,  C12N5/00 ,  C12N5/071 ,  C12N15/63 ,  C12N15/85

    CPC分类号： C07K14/005 ,  C07K2319/20 ,  C12N2770/24222 ,  G01N33/5767 ,  G01N2333/183 ,  G01N2500/10

    摘要： Cells expressing chimeric hepatitis C virus NS4A protein are disclosed, as are vectors and methods for preparing such cells and methods of using the cells and the chimeric proteins therein in screening assays for detecting compounds that alter the associative characteristics of NS4A proteins. Disclosed screening assays are suitable for use in high throughput screening of compound libraries to identify compounds with a exhibiting specific anti-viral activity against hepatitis C virus.

    摘要翻译： 公开了表达嵌合丙型肝炎病毒NS4A蛋白的细胞，以及用于制备这种细胞的载体和方法以及其中使用细胞和嵌合蛋白的筛选测定法的方法，用于检测改变NS4A蛋白的缔合特性的化合物。 公开的筛选测定法适用于化合物文库的高通量筛选以鉴定具有针对丙型肝炎病毒的特异性抗病毒活性的化合物。

    SUBSTITUTED TARAXASTANES USEFUL FOR TREATING VIRAL INFECTIONS
    7.
    发明申请
    SUBSTITUTED TARAXASTANES USEFUL FOR TREATING VIRAL INFECTIONS 审中-公开
    替代治疗药物可用于治疗病毒感染

    公开(公告)号：US20070197646A1

    公开(公告)日：2007-08-23

    申请号：US11676803

    申请日：2007-02-20

    申请人： Barton James BRADBURY ,  Mingjun Huang

    发明人： Barton James BRADBURY ,  Mingjun Huang

    IPC分类号： A61K31/203 ,  C07C61/29

    CPC分类号： C07J63/008 ,  C07C62/32

    摘要： Substituted taraxastanes useful for treating viral infections, are provided herein. Thus, in a first aspect, the invention provides compounds of Formula I and the pharmaceutically acceptable salts thereof, wherein the variables R1, R2, and X are defined herein. The compounds described herein are thought to act by inhibiting retroviral maturation, including maturation of encapsulated retroviruses viruses, such as the HIV viruses, HIV-1 and HIV-2. Pharmaceutical compositions comprising such compounds of Formula I are included herein. Methods of using such compounds to treat human patients infected with an HIV virus and reducing the mortality of AIDS are also provided herein.

    摘要翻译： 本文提供了可用于治疗病毒感染的替代的塔西沙星。 因此，在第一方面，本发明提供式I化合物及其药学上可接受的盐，其中R 1，R 2和X 2的定义在本文中。 本文所述的化合物被认为通过抑制逆转录病毒成熟（包括成熟的复制病毒，如HIV病毒，HIV-1和HIV-2）而起作用。 包含式I化合物的药物组合物包括在本文中。 本文还提供了使用这些化合物治疗感染HIV病毒的患者和降低AIDS死亡率的方法。

    Pharmaceutical compositions for and methods of inhibiting HCV replication
    8.
    发明申请
    Pharmaceutical compositions for and methods of inhibiting HCV replication 审中-公开
    用于抑制HCV复制的药物组合物和方法

    公开(公告)号：US20090081636A1

    公开(公告)日：2009-03-26

    申请号：US11401408

    申请日：2006-04-11

    申请人： Mingjun Huang

    发明人： Mingjun Huang

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/5067 ,  C12N2770/24211 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5767

    摘要： The present invention relates generally to replicase complex defect inducers and pharmaceutical compositions containing such inducers. Methods of developing mutants that are resistant to replicase complex defect inducers are also provided. Further included are mutants that can be used in screening for replicase complex defect inducers. Methods of screening test compounds for the ability to induce the formation of replicase complex defects are also described. Also included are methods of inhibition of HCV replication by replicase complex defect inducers.

    摘要翻译： 本发明一般涉及复制酶复合缺陷诱导剂和含有该诱导剂的药物组合物。 还提供了开发对复制酶复合缺陷诱导物具有抗性的突变体的方法。 还包括可用于筛选复制酶复合缺陷诱导物的突变体。 还描述了筛选测试化合物诱导复制酶复合物缺陷形成能力的方法。 还包括通过复制酶复合缺陷诱导剂抑制HCV复制的方法。

    Pharmaceutical Compositions For and Methods of Inhibiting Hcv
    9.
    发明申请
    Pharmaceutical Compositions For and Methods of Inhibiting Hcv 审中-公开
    药物组合物和抑制Hcv的方法

    公开(公告)号：US20080207760A1

    公开(公告)日：2008-08-28

    申请号：US11911330

    申请日：2006-04-11

    申请人： Mingjun Huang

    发明人： Mingjun Huang

    IPC分类号： A61K31/17 ,  C12Q1/70 ,  C07C335/00 ,  A61P31/14

    CPC分类号： G01N33/5067 ,  C12N2770/24211 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5767

    摘要： The present invention relates generally to replicase complex defect inducers and pharmaceutical compositions containing such inducers. Methods of developing mutants that are resistant to replicase complex defect inducers are also provided. Further included are mutants that can be used in screening for replicase complex defect inducers. Methods of screening test compounds for the ability to induce the formation of replicase complex defects are also described. Also included are methods of inhibition of HCV replication by replicase complex defect inducers.

    摘要翻译： 本发明一般涉及复制酶复合缺陷诱导剂和含有该诱导剂的药物组合物。 还提供了开发对复制酶复合缺陷诱导物具有抗性的突变体的方法。 还包括可用于筛选复制酶复合缺陷诱导物的突变体。 还描述了筛选测试化合物诱导复制酶复合物缺陷形成能力的方法。 还包括通过复制酶复合缺陷诱导剂抑制HCV复制的方法。