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公开(公告)号：US5856163A
公开(公告)日：1999-01-05
申请号：US347335
申请日：1994-12-22
申请人： Miyoko Hashida , Masanobu Abo , Yukiko Takamura , Ole Kirk , Torben Halkier , Sven Pedersen , Shamkant Anant Patkar , Mogens Trier Hansen
发明人： Miyoko Hashida , Masanobu Abo , Yukiko Takamura , Ole Kirk , Torben Halkier , Sven Pedersen , Shamkant Anant Patkar , Mogens Trier Hansen
IPC分类号： C12N15/09 , C12N1/14 , C12N1/15 , C12N9/20 , C12N11/02 , C12P19/02 , C12R1/645 , C12R1/69 , C14C1/00 , D21C5/00 , D21H11/02 , D21H21/02 , D21C3/00
摘要： A new species of the genus Hyphogyma is disclosed, as are new lipases obtainable therefrom, Also disclosed is a process for obtaining the enzymes.
摘要翻译： PCT No.PCT / DK93 / 00194 Sec。 371日期1994年12月22日第 102（e）日期1994年12月22日PCT Filed 1993年6月3日PCT公布。 公开号WO93 / 24619 日期：1993年12月9日公开了一种新的嗜血杆菌属，以及由此获得的新脂肪酶。还公开了获得酶的方法。
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公开(公告)号：US6020180A
公开(公告)日：2000-02-01
申请号：US111556
申请日：1998-07-08
申请人： Allan Svendsen , Shamkant Anant Patkar , Michi Egel-Mitani , Kim Borch , Ib Groth Clausen , Mogens Trier Hansen
发明人： Allan Svendsen , Shamkant Anant Patkar , Michi Egel-Mitani , Kim Borch , Ib Groth Clausen , Mogens Trier Hansen
IPC分类号： C12N15/09 , C12N1/19 , C12N1/21 , C12N9/20 , C12N15/55 , C12P7/64 , C12R1/19 , C12R1/69 , C12S3/08 , D21C9/08 , D21H21/02 , C07H21/04 , C12N15/63
CPC分类号： D21H21/02 , C12N9/20 , C07K2299/00
摘要： A DNA sequence encoding a lipase having the amino acid sequence shown in SEQ ID NO:2. The lipase has a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant of the amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase.
摘要翻译： 编码具有SEQ ID NO：2所示的氨基酸序列的脂肪酶的DNA序列。 脂肪酶具有胰蛋白酶样催化三单元组，其包括位于脂肪酶分子的主要疏水性，细长结合口袋中的活性丝氨酸，并且位于脂肪酶结构的脂质接触区的临界位置，不同于 芳族氨基酸残基，其中氨基酸残基在水解期间或期间与脂质底物相互作用，其中氨基酸残基的脂肪酶变体已经被芳族氨基酸残基取代，从而赋予变体比增加的比活性 与母体脂肪酶的相同。
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公开(公告)号：US06413559B1
公开(公告)日：2002-07-02
申请号：US09718709
申请日：2000-11-22
申请人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek Østergaard , Shamkant Anant Patkar , Kim Hansen
发明人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek Østergaard , Shamkant Anant Patkar , Kim Hansen
IPC分类号： A21D200
摘要： The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.
摘要翻译： 本发明涉及一种表现出来自真菌微生物的氨基肽酶活性的35kDa酶，包含编码所述酶的DNA序列的DNA构建体，包含所述DNA构建体的重组表达载体和包含所述DNA序列的细胞。 本发明还提供了生产表现出氨基肽酶活性的酶的方法，以及包含所述酶的酶制剂，包含本发明的氨基肽酶的面包改良组合物或面团改良组合物。 最后，本发明涉及所述显示氨基肽酶活性的酶或酶制剂或其组合物的用途。
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公开(公告)号：US5994113A
公开(公告)日：1999-11-30
申请号：US929922
申请日：1997-09-15
申请人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
发明人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
IPC分类号： G02C13/00 , A21D8/04 , A23J3/34 , C07H21/04 , C11D3/386 , C11D7/42 , C12N1/16 , C12N1/19 , C12N9/48 , C12N15/00 , C12N15/09 , C12R1/19 , C12R1/69
摘要： The present invention relates to a enzyme exhibiting aminopeptidase activity, a method for producting said enzyme, and enzyme preparation containing said enzyme exhibithing aminopeptidase activity, and use of said enzyme for various industrial purposes.
摘要翻译： 本发明涉及一种显示氨基肽酶活性的酶，一种产生所述酶的方法，以及含有展示氨基肽酶活性的酶的酶制剂，以及所述酶用于各种工业目的。
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公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞 序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US06495357B1
公开(公告)日：2002-12-17
申请号：US09007288
申请日：1998-01-14
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号： C12N920
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及一种具有脂肪分解活性的修饰酶，能够一周期洗涤大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA序列的宿主细胞 或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US06200792B1
公开(公告)日：2001-03-13
申请号：US09580064
申请日：2000-05-30
申请人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek Østergaard , Shamkant Anant Patkar , Kim Hansen
发明人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek Østergaard , Shamkant Anant Patkar , Kim Hansen
IPC分类号： C12N948
摘要： The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.
摘要翻译： 本发明涉及一种表现出氨基肽酶活性的酶，一种生产该酶的方法，一种含有显示氨基肽酶活性的酶的酶制剂，以及所述酶用于各种工业目的。
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公开(公告)号：US6143546A
公开(公告)日：2000-11-07
申请号：US342394
申请日：1999-06-29
申请人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
发明人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
IPC分类号： G02C13/00 , A21D8/04 , A23J3/34 , C07H21/04 , C11D3/386 , C11D7/42 , C12N1/16 , C12N1/19 , C12N9/48 , C12N15/00 , C12N15/09 , C12R1/19 , C12R1/69
摘要： The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.
摘要翻译： 本发明涉及一种表现出氨基肽酶活性的酶，一种生产该酶的方法，一种含有显示氨基肽酶活性的酶的酶制剂，以及所述酶用于各种工业目的。
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公开(公告)号：US6143545A
公开(公告)日：2000-11-07
申请号：US387922
申请日：1999-09-01
申请人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
发明人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
CPC分类号： C11B3/003 , A21D8/042 , A23J7/00 , A23K20/189
摘要： The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorus content, wherein said method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of said enzyme for a number of industrial applications.
摘要翻译： 本发明涉及一种降低食用油中含磷成分含量的方法，该方法包括大量不可水合的磷含量，其中所述方法包括使用磷脂酶。 此外，本发明涉及具有磷脂酶活性的酶，编码具有磷脂酶活性的酶的克隆DNA序列，该酶的制备方法以及所述酶在许多工业应用中的用途。
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公开(公告)号：US06103505A
公开(公告)日：2000-08-15
申请号：US988111
申请日：1997-12-09
申请人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
发明人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
CPC分类号： C11B3/003 , A21D8/042 , A23J7/00 , A23K20/189
摘要： The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorous content, wherein the method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of enzyme for a number of industrial applications.
摘要翻译： 本发明涉及一种降低食用油中含磷成分含量的方法，该方法包括大量不可水合的磷含量，其中该方法包括使用磷脂酶。 此外，本发明涉及具有磷脂酶活性的酶，编码具有磷脂酶活性的酶的克隆DNA序列，该酶的制备方法和用于多种工业应用的酶的用途。
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