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    Protein structure analysis method, protein structure analyzing instrument, program and recording medium
    1.
    发明申请
    Protein structure analysis method, protein structure analyzing instrument, program and recording medium 审中-公开
    蛋白质结构分析方法，蛋白质结构分析仪器，程序和记录介质

    公开(公告)号：US20090319234A1

    公开(公告)日：2009-12-24

    申请号：US10973096

    申请日：2004-10-26

    申请人： Naoyuki Yamada ,  Eiichiro Suzuki ,  Kazuo Hirayama ,  Hideaki Umeyama

    发明人： Naoyuki Yamada ,  Eiichiro Suzuki ,  Kazuo Hirayama ,  Hideaki Umeyama

    IPC分类号： G06F17/50

    CPC分类号： G01N33/6848 ,  C07K1/00 ,  G01N33/68 ,  G01N33/6851

    摘要： This invention fragments ions ionized by an electro-spray ionization method or the like for a target protein whose three-dimensional structure is to be predicted, to fragment ions by a hexapole CID method or the like, and measures a fragmentation spectrum. The present invention determines fragment ion assignment information on an amino acid sequence of the target protein based on the measured fragmentation spectrum. The present invention specifies a region of the amino acid sequence of the target protein in which region the ions are dissociated to the fragment ions according to the determined fragment ion assignment information, and determines easily cleavable domain information on the amino acid sequence of the target protein according to the specified region. The present invention predicts the three-dimensional structure of the target protein. The present invention outputs predicted three-dimensional structure prediction data and the determined easily cleavable domain information while making them correspond to each other.

    摘要翻译： 本发明通过电喷雾离子化法等离子化对于要预测其三维结构的靶蛋白进行离子化离子化，通过六极CID法等分离离子，测定碎裂光谱。 本发明基于测定的断裂谱，确定靶蛋白的氨基酸序列的片段离子分配信息。 本发明根据确定的片段离子分配信息指定靶蛋白的氨基酸序列的区域，其中离子被离解为片段离子，并且确定易于切割的关于靶蛋白的氨基酸序列的结构域信息 根据指定区域。 本发明预测了靶蛋白的三维结构。 本发明输出预测的三维结构预测数据和确定的容易切割的域信息，同时使它们彼此对应。

    Polynucleotide encoding a mutant transglutaminase
    3.
    发明授权
    Polynucleotide encoding a mutant transglutaminase 失效
    编码突变型转谷氨酰胺酶的多核苷酸

    公开(公告)号：US07553650B2

    公开(公告)日：2009-06-30

    申请号：US11505438

    申请日：2006-08-17

    申请人： Tatsuki Kashiwagi ,  Nobuhisa Shimba ,  Kohki Ishikawa ,  Ei-ichiro Suzuki ,  Keiichi Yokoyama ,  Kazuo Hirayama

    发明人： Tatsuki Kashiwagi ,  Nobuhisa Shimba ,  Kohki Ishikawa ,  Ei-ichiro Suzuki ,  Keiichi Yokoyama ,  Kazuo Hirayama

    IPC分类号： C12N9/10 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/1044 ,  C07K14/195 ,  C07K2299/00 ,  C12N15/102 ,  C12Y203/02013

    摘要： The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.

    摘要翻译： 本发明涉及基于来自链霉菌（MTG）的MTG的三维结构和由此制备的突变体MTG来设计和制备突变转谷氨酰胺酶的方法。 本发明提供了一种基于三维结构修饰MTG的方法，并且通过该方法改善了在基板上具有反应性的转谷氨酰胺酶。 在本发明中，基于通过MTG晶体的X射线晶体结构分析获得的三维结构外推基质的MTG的结合位点，通过置换，插入或缺失氨基酸来设计和生产突变型转谷氨酰胺酶 位于转谷氨酰胺酶底物结合位点的残基。

    1-Substituted 4-carbamoyl-1,2,4-triazol-5-one derivatives and herbicide
    4.
    发明授权
    1-Substituted 4-carbamoyl-1,2,4-triazol-5-one derivatives and herbicide 有权
    1-取代的4-氨基甲酰基-1,2,4-三唑-5-酮衍生物和除草剂

    公开(公告)号：US6077814A

    公开(公告)日：2000-06-20

    申请号：US367822

    申请日：1999-08-23

    申请人： Ken Morita ,  Tsunehiro Kido ,  Kazuo Hirayama ,  Hiroyuki Okita ,  Toshiharu Ohno ,  Yoshihisa Watanabe ,  Masahide Onoe

    发明人： Ken Morita ,  Tsunehiro Kido ,  Kazuo Hirayama ,  Hiroyuki Okita ,  Toshiharu Ohno ,  Yoshihisa Watanabe ,  Masahide Onoe

    IPC分类号： A01N47/38 ,  C07D249/12 ,  C07D401/12 ,  A01N35/04 ,  A01N43/653

    CPC分类号： C07D401/12 ,  A01N47/38 ,  C07D249/12

    摘要： A 1-phenyl, naphthyl or aralkyl-4-(N,N-di-substituted carbamoyl)-1,2,4-triazol-5-one derivative represented by the general formula (I) ##STR1## wherein A is an unsubstituted or substituted phenyl group, 1-naphthyl group, 5,6,7,8-tetrahydro-1-naphthyl group or an aralkyl group such as an unsubstituted or substituted benzyl group and so on, R.sub.1 is a lower alkyl group and so on, and R.sub.2 is an unsubstituted or substituted phenyl group and so on, was prepared as novel compounds. This 1-substituted-4-carbamoyl-1,2,4-triazol-5-one derivative does not substantially exhibit phytotoxicity to various agricultural crop plants and therefore is useful as a selective herbicide.

    摘要翻译： PCT No.PCT / JP98 / 00803 Sec。 371日期1999年8月23日 102（e）日期1999年8月23日PCT提交1998年2月26日PCT公布。 公开号WO98 / 38176 日期：1998年9月3日由通式（I）表示的1-苯基，萘基或芳烷基-4-（N，N-二取代氨基甲酰基）-1,2,4-三唑-5-酮衍生物，其中A为 未取代或取代的苯基，1-萘基，5,6,7,8-四氢-1-萘基或芳烷基如未取代或取代的苄基等，R 1为低级烷基等 并且R2是未取代或取代的苯基等，作为新化合物制备。 该1-取代-4-氨基甲酰基-1,2,4-三唑-5-酮衍生物基本上不显示对各种农作物植物的植物毒性，因此可用作选择性除草剂。

    Method for modifying transglutaminases from microorganisms
    9.
    发明申请
    Method for modifying transglutaminases from microorganisms 失效
    从微生物修饰转谷氨酰胺酶的方法

    公开(公告)号：US20060275872A1

    公开(公告)日：2006-12-07

    申请号：US11505438

    申请日：2006-08-17

    申请人： Tatsuki Kashiwagi ,  Nobuhisa Shimba ,  Kohki Ishikawa ,  Ei-ichiro Suzuki ,  Keiichi Yokoyama ,  Kazuo Hirayama

    发明人： Tatsuki Kashiwagi ,  Nobuhisa Shimba ,  Kohki Ishikawa ,  Ei-ichiro Suzuki ,  Keiichi Yokoyama ,  Kazuo Hirayama

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/10

    CPC分类号： C12N9/1044 ,  C07K14/195 ,  C07K2299/00 ,  C12N15/102 ,  C12Y203/02013

    摘要： The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.

    摘要翻译： 本发明涉及基于来自链霉菌（MTG）的MTG的三维结构和由此制备的突变体MTG来设计和制备突变转谷氨酰胺酶的方法。 本发明提供了一种基于三维结构修饰MTG的方法，并且通过该方法改善了在基板上具有反应性的转谷氨酰胺酶。 在本发明中，基于通过MTG晶体的X射线晶体结构分析获得的三维结构外推基质的MTG的结合位点，通过置换，插入或缺失氨基酸来设计和生产突变型转谷氨酰胺酶 位于转谷氨酰胺酶底物结合位点的残基。