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    NUCLEIC ACID DETECTION
    2.
    发明申请
    NUCLEIC ACID DETECTION 审中-公开
    核酸检测

    公开(公告)号：US20120183965A1

    公开(公告)日：2012-07-19

    申请号：US13261083

    申请日：2010-06-14

    申请人： David Ward ,  David Edge ,  Nelson Nazareth

    发明人： David Ward ,  David Edge ,  Nelson Nazareth

    IPC分类号： G01N21/64 ,  C12M1/40 ,  G01N21/17

    CPC分类号： G01N21/6428 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2400/0421 ,  C12Q1/686 ,  G01N21/645 ,  G01N27/447 ,  G01N27/44726 ,  G01N2021/0346 ,  G01N2021/6439 ,  G01N2021/6482 ,  C12Q2565/125 ,  C12Q2565/102 ,  C12Q2537/143

    摘要： Apparatus for detecting a plurality of analytes in a sample and comprising: a reaction vessel; means for subjecting a sample in the reaction vessel to a multiplication process; a separation stage operable to separate amplimer constituents according to size; optical detection means for quantifying the sizes present and determining the colour of each size; and means for comparing the resulting quantification and colours with known data to determine the nature of the or each or a proportion of the or each target amplimers present. Processes employing the apparatus are also described.

    摘要翻译： 用于检测样品中多种分析物的装置，包括：反应容器; 用于使反应容器中的样品经受乘法的装置; 分离阶段，可操作以根据尺寸分离放大成分; 光学检测装置，用于量化存在的尺寸并确定每种尺寸的颜色; 以及用于将得到的定量和颜色与已知数据进行比较以确定存在的或每个或每个目标扩增子的比例的性质的装置。 还描述了使用该装置的过程。

    Apparatus for and methods of the disruption of a biological cell
    8.
    发明授权
    Apparatus for and methods of the disruption of a biological cell 有权

    公开(公告)号：US11447767B2

    公开(公告)日：2022-09-20

    申请号：US13261540

    申请日：2011-06-15

    申请人： Nelson Nazareth ,  David Ward ,  David Edge

    发明人： Nelson Nazareth ,  David Ward ,  David Edge

    IPC分类号： C12N15/10 ,  B01L7/00 ,  C12N1/06 ,  F25B21/04 ,  C12N1/08 ,  B01L3/00

    摘要： Disrupting a biological cell includes freezing, boiling or perhaps alternately freezing and boiling material containing the biological cell using a thermoelectric cell with a working face, and a base face whereof is contiguous with a heat source/sink at a substantially constant temperature. Apparatus for the disruption process includes a peltier cell, a base face, which is flexibly attached to a heat source/sink held at a constant temperature, and a working face contiguous with a reaction vessel or holder thereof. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel or holder for heating, with the TEC used purely for cooling. The materials of the base face tend to inhibit disintegration of the peltier cell brought about by expansion and contraction by heat.

    CELL DISRUPTION
    9.
    发明申请
    CELL DISRUPTION 审中-公开
    细胞破坏

    公开(公告)号：US20130115670A1

    公开(公告)日：2013-05-09

    申请号：US13261540

    申请日：2011-06-15

    申请人： Nelson Nazareth ,  David Ward ,  David Edge

    发明人： Nelson Nazareth ,  David Ward ,  David Edge

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1003 ,  B01L3/5029 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/044 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  C12N1/066 ,  C12N1/08 ,  F25B21/04

    摘要： A process for the disruption of a biological cell comprising freezing, boiling or perhaps alternately freezing and boiling the material containing the biological cell using a thermoelectric cell a base face whereof is contiguous with a heat sink/source held at a substantially constant temperature and a working face . Apparatus for carrying out the disruption process comprises a peltier cell a base face of which is flexibly attached to a heat sink arranged to be kept at a constant temperature of around 50° C. and a working face of which is contiguous with a reaction vessel or a reaction vessel holder. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel holder for heating with the TEC used purely for cooling The peltier cell base face is constructed of materials which tend to inhibit disintegration of the peltier cell brought about by expansion and contraction under heat.

    摘要翻译： 一种用于破坏生物细胞的方法，包括使用热电池冷冻，沸腾或者可能交替地冷冻和沸腾含有生物细胞的材料，其基面与保持在基本上恒定的温度的热沉/源连接，并且工作 脸。 用于进行破碎过程的装置包括一个珀耳帖细胞，其基面被柔性地附接到散热器，该散热器被设置成保持在约50℃的恒定温度，其工作面与反应容器相连， 反应容器支架。 在皮肤细胞中的电压反转使得工作面交替地达到低于冰点和沸点以上的温度，和/或使用电阻丝在容器保持器上用于纯化用于冷却的TEC加热珀耳帖细胞基面是 由倾向于抑制由加热引起的膨胀和收缩引起的珀耳帖细胞崩解的材料构成。

    THERMAL CYCLING APPARATUS AND PROCESS
    10.
    发明申请
    THERMAL CYCLING APPARATUS AND PROCESS 审中-公开
    热循环装置和工艺

    公开(公告)号：US20100203595A1

    公开(公告)日：2010-08-12

    申请号：US12450028

    申请日：2008-03-06

    申请人： David Ward ,  Nelson Nazareth

    发明人： David Ward ,  Nelson Nazareth

    IPC分类号： C12P19/34 ,  F28F13/06 ,  F25B21/04 ,  C12M1/00 ,  C12M1/34

    CPC分类号： F25B21/04 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/147 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/185

    摘要： A thermal cycling apparatus 9 and process includes at least one reaction vessel 14 which is associated with a thermoelectric cooler 12 (TEC), such as a Peltier cell, and arranged to provide both heating and cooling of the reaction vessel. A first side of the TEC 12 is associated with the at least one reaction vessel 14 and a second side of the TEC is arranged in use to be maintained at a temperature intermediate the highest temperature and the lowest temperature used in a thermal cycling operation. Electric current is supplied to the TEC 12 in one direction whereby the said first side becomes hotter than the second side, and then in the other direction whereby the first side becomes cooler than the second side.

    摘要翻译： 热循环装置9和方法包括至少一个与热电冷却器12（TEC）相关联的反应容器14，例如珀尔帖（Peltier）电池，并且布置成提供反应容器的加热和冷却。 TEC 12的第一侧与至少一个反应容器14相关联，TEC的第二侧被布置在使用中以保持在热循环操作中使用的最高温度和最低温度之间的温度。 在一个方向上向TEC12供给电流，由此所述第一侧变得比第二侧更热，然后在另一个方向上使第一侧变得比第二侧更冷。