公开(公告)号：US20070184449A1

公开(公告)日：2007-08-09

申请号：US10591087

申请日：2005-03-22

申请人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

发明人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

IPC分类号： C40B40/02 ,  C40B40/08 ,  C40B50/06

CPC分类号： C12P7/40 ,  C12N2503/02 ,  C12P7/02 ,  C12P13/02 ,  C12P33/00

摘要： The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.

摘要翻译： 本发明涉及在将底物{Ai}转化为目标产物{B}中具有确定功能的代谢途径的直接选择，其在工业，制药或农业食品领域是感兴趣的。 更具体地，本发明涉及在宏基因组文库中检测参与具有已知产物{B}的生化反应的新生物合成途径。 所述新型代谢途径的选择和表征使得{B}可以酶促产生。 本发明提供了所述分子的化学合成（B）的替代方案。 此外，最重要的是，本发明可以专门用于靶向和利用唯一能够将{Ai}转化为{B}的代谢途径，同时消除可能分解目标产物{B}的相关代谢途径。

公开(公告)号：US07846874B2

公开(公告)日：2010-12-07

申请号：US10591087

申请日：2005-03-22

申请人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

发明人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

IPC分类号： C40B30/04 ,  C40B20/04

CPC分类号： C12P7/40 ,  C12N2503/02 ,  C12P7/02 ,  C12P13/02 ,  C12P33/00

摘要： The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterization of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.

摘要翻译： 本发明涉及在将底物{Ai}转化为目标产物{B}中具有确定功能的代谢途径的直接选择，其在工业，制药或农业食品领域是感兴趣的。 更具体地，本发明涉及在宏基因组文库中检测参与具有已知产物{B}的生化反应的新生物合成途径。 所述新型代谢途径的选择和表征使得{B}可以酶促产生。 本发明提供了所述分子的化学合成（B）的替代方案。 此外，最重要的是，本发明可以专门用于靶向和利用唯一能够将{Ai}转化为{B}的代谢途径，同时消除可能分解目标产物{B}的相关代谢途径。

公开(公告)号：US20100323390A1

公开(公告)日：2010-12-23

申请号：US12872280

申请日：2010-08-31

申请人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

发明人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

IPC分类号： C12Q1/02 ,  C40B30/06 ,  C12P33/02 ,  C12P7/22 ,  C12P7/42 ,  C12P13/02

CPC分类号： C12P7/40 ,  C12N2503/02 ,  C12P7/02 ,  C12P13/02 ,  C12P33/00

摘要： The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.

摘要翻译： 本发明涉及在将底物{Ai}转化为目标产物{B}中具有确定功能的代谢途径的直接选择，其在工业，制药或农业食品领域是感兴趣的。 更具体地，本发明涉及在宏基因组文库中检测参与具有已知产物{B}的生化反应的新生物合成途径。 所述新型代谢途径的选择和表征使得{B}可以酶促产生。 本发明提供了所述分子的化学合成（B）的替代方案。 此外，最重要的是，本发明可以专门用于靶向和利用唯一能够将{Ai}转化为{B}的代谢途径，同时消除可能分解目标产物{B}的相关代谢途径。

公开(公告)号：US07910522B2

公开(公告)日：2011-03-22

申请号：US10522037

申请日：2003-07-17

申请人： Renaud Nalin ,  Petar Pujic ,  Karine Tuphile ,  Benjamin Gillet

发明人： Renaud Nalin ,  Petar Pujic ,  Karine Tuphile ,  Benjamin Gillet

IPC分类号： C40B50/06 ,  C12Q1/68

CPC分类号： C07K14/195 ,  C07H21/00 ,  C12N9/22 ,  C12N15/1034 ,  C12N15/1079 ,  C12N15/1086 ,  C12N15/70 ,  C12N15/76

摘要： The present invention relates to methods for the identification or cloning of polynucleotides encoding a selected phenotype, particularly from environmental DNA. In a specific embodiment, the method comprises (i) cloning environmental DNA fragments into E. coli cloning vectors to produce a metagenomic library, (ii) identifying or selecting cloning vectors in the library which contain DNA fragments having a particular characteristic of interest, (iii) modifying the identified or selected cloning vectors into shuttle or expression vectors for transfer and integration in a selected host cell, (iv) transferring the modified cloning vectors into the selected host-cell and (v) identifying or cloning the DNA fragments contained in the modified cloning vectors which encode the selected phenotype in the selected host cell.

摘要翻译： 本发明涉及用于鉴定或克隆编码所选表型的多核苷酸，特别是环境DNA的方法。 在一个具体实施方案中，所述方法包括（i）将环境DNA片段克隆到大肠杆菌克隆载体中以产生宏基因组文库，（ii）在文库中鉴定或选择含有具有特定特征特征的DNA片段的克隆载体（ iii）将所鉴定或选择的克隆载体修饰为穿梭或表达载体，用于转移和整合到所选择的宿主细胞中，（iv）将修饰的克隆载体转移到所选择的宿主细胞中，以及（v）鉴定或克隆含有 编码选择的宿主细胞中选择的表型的修饰的克隆载体。

公开(公告)号：US20050282166A1

公开(公告)日：2005-12-22

申请号：US10522037

申请日：2003-07-17

申请人： Renaud Nalin ,  Petar Pujic ,  Karine Tuphile ,  Benjamin Gillet

发明人： Renaud Nalin ,  Petar Pujic ,  Karine Tuphile ,  Benjamin Gillet

IPC分类号： C07H21/00 ,  C07K14/00 ,  C07K14/195 ,  C12N9/22 ,  C12N15/10 ,  C12N15/70 ,  C12N15/76 ,  C12Q1/68 ,  C12N15/74

CPC分类号： C07K14/195 ,  C07H21/00 ,  C12N9/22 ,  C12N15/1034 ,  C12N15/1079 ,  C12N15/1086 ,  C12N15/70 ,  C12N15/76

摘要： The present invention relates to methods for the identification or cloning of polynucleotides encoding a selected phenotype, particularly from environmental DNA. In a specific embodiment, the method comprises (i) cloning environmental DNA fragments into E coli cloning vectors to produce a metagenomic library, (ii) identifying or selecting cloning vectors in said library which contain DNA fragments having a particular characteristic of interest, (iii) modifying the identified or selected cloning vectors into shuttle or expression vectors for transfer and integration in a selected host cell, (iv) transferring the modified cloning vectors into said selected host-cell and (v) identifying or cloning the DNA fragments contained in said modified cloning vectors which encode said selected phenotype in said selected host cell.

摘要翻译： 本发明涉及用于鉴定或克隆编码所选表型的多核苷酸，特别是环境DNA的方法。 在一个具体实施方案中，所述方法包括（i）将环境DNA片段克隆到大肠杆菌克隆载体中以产生宏基因组文库，（ii）在所述文库中鉴定或选择含有具有特定特征特征的DNA片段的克隆载体， 将所鉴定或选择的克隆载体修饰为穿梭或表达载体，以转移和整合到所选择的宿主细胞中，（iv）将修饰的克隆载体转移到所述选择的宿主细胞中，以及（v）鉴定或克隆所述 修饰的克隆载体，其在所述选择的宿主细胞中编码所述选择的表型。

公开(公告)号：US20110183930A1

公开(公告)日：2011-07-28

申请号：US13052633

申请日：2011-03-21

申请人： DANIEL AURIOL ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

发明人： DANIEL AURIOL ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

IPC分类号： A61K31/7048 ,  C12P19/60 ,  C07H17/07 ,  A61P35/00 ,  A61P9/00 ,  A61P31/04 ,  A61P37/08 ,  C12P19/44 ,  C07H15/203 ,  A61K31/7034

CPC分类号： C07H17/075 ,  C07H15/203 ,  C07H15/24 ,  C07H15/26 ,  C12P17/02 ,  C12P19/02 ,  C12P19/44 ,  C12P19/60

摘要： The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-α-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic, antimutagenic, antineoplastic, anti-thrombotic and vasodilatory formulations, or in any other field of application.

摘要翻译： 本发明涉及通过将选自邻苯二酚或其衍生物的酚类与蔗糖的葡萄糖部分的酶缩合制备酚衍生物。 所述酚衍生物的生产通过葡糖基转移酶（EC 2.4.1.5）来实现。 所选择的酚类的这些O-α-葡萄糖苷是新的，在水中的溶解度高于其亲本多酚的溶解度，并且在化妆品和药物组合物中有用，例如抗氧化，抗病毒，抗菌，免疫刺激，抗过敏，抗高血压， 抗血栓形成，抗血栓形成，抗血栓形成，抗血栓形成，血栓形成和抗血栓形成，抗血栓形成，抗血栓形成，抗血栓形成，抗胆固醇血症，抗脂质过氧化物，肝保护，抗炎，抗致癌，​​抗诱变，抗肿瘤，抗血栓形成和血管扩张制剂。

公开(公告)号：US20060188967A1

公开(公告)日：2006-08-24

申请号：US11338364

申请日：2006-01-24

申请人： Renaud Nalin ,  Patrick Robe ,  Van Van

发明人： Renaud Nalin ,  Patrick Robe ,  Van Van

IPC分类号： C12P19/34

CPC分类号： G01N27/44773 ,  C12N15/1003

摘要： A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.

摘要翻译： 公开了一种从环境样品中所含的不可培养生物体间接提取尺寸大于300kb的DNA的方法。 该方法包括从样品中分离生物体并将分离的生物体包埋在琼脂糖块中，随后将生物体裂解，DNA进行第一次交替场电泳以提取尺寸较大的DNA片段并将其与细胞分离 碎片 第一次电泳迁移之后是酶限制步骤和额外的电泳迁移。 本发明还包括通过该方法获得的DNA。

公开(公告)号：US20090233876A1

公开(公告)日：2009-09-17

申请号：US12304212

申请日：2007-06-13

申请人： Daniel Auriol ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

发明人： Daniel Auriol ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

IPC分类号： A61K31/7048 ,  C12P19/60 ,  C07H17/06 ,  A61P35/00

CPC分类号： C07H17/075 ,  C07H15/203 ,  C07H15/24 ,  C07H15/26 ,  C12P17/02 ,  C12P19/02 ,  C12P19/44 ,  C12P19/60

摘要： The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-α-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrhythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic antimutagenic, antineoplastic, anti-thrombotic, and vasodilatory formulations, or in any other field of application.

摘要翻译： 本发明涉及通过将选自邻苯二酚或其衍生物的酚类与蔗糖的葡萄糖部分的酶缩合制备酚衍生物。 所述酚衍生物的生产通过葡糖基转移酶（EC 2.4.1.5）来实现。 所选择的酚类的这些O-α-葡萄糖苷是新的，在水中的溶解度高于其亲本多酚的溶解度，并且在化妆品和药物组合物中有用，例如抗氧化，抗病毒，抗菌，免疫刺激，抗过敏，抗高血压， 抗缺血性，抗心律不齐，抗血栓形成，低胆固醇血症，抗脂质过氧化物，肝保护，抗炎，抗致癌性抗诱发剂，抗肿瘤剂，抗血栓形成和血管扩张制剂，或在任何其他应用领域。

公开(公告)号：US06989249B2

公开(公告)日：2006-01-24

申请号：US10282190

申请日：2002-10-28

申请人： Renaud Nalin ,  Patrick Robe ,  Van Tran Van

发明人： Renaud Nalin ,  Patrick Robe ,  Van Tran Van

IPC分类号： C12P19/34 ,  C12N1/08 ,  C07H21/00 ,  C07H1/08

CPC分类号： G01N27/44773 ,  C12N15/1003

摘要： A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.

公开(公告)号：US20100015667A1

公开(公告)日：2010-01-21

申请号：US12295364

申请日：2007-04-03

申请人： Fabrice Lefevre ,  Renaud Nalin ,  Cyrille Jarrin

发明人： Fabrice Lefevre ,  Renaud Nalin ,  Cyrille Jarrin

IPC分类号： C12P19/34 ,  C12N15/66

CPC分类号： C12N15/1027

摘要： The present invention relates to a method for in vitro recombining polynucleotides sequences of interest from at least two initial different polynucleotide sequences of interest by cleavage/ligation of circular DNA molecules.

摘要翻译： 本发明涉及通过切割/连接环状DNA分子来从至少两个初始不同的感兴趣的多核苷酸序列体外重组感兴趣的多核苷酸序列的方法。