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    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION
    1.
    发明申请
    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION 审中-公开
    DROPLET标记和放大的方法和系统

    公开(公告)号：US20170029813A1

    公开(公告)日：2017-02-02

    申请号：US15303893

    申请日：2015-04-17

    申请人： President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusets General Hospital

    发明人： David A. Weitz ,  Anthony John Iafrate ,  Zongli Zheng ,  Huidan Zhang

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    摘要： The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfiuidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

    摘要翻译： 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过将多个核酸“标签”并入可选地扩增核酸，例如在液滴内，液滴暴露于的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

    MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS
    6.
    发明申请
    MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS 有权

    公开(公告)号：US20230078810A1

    公开(公告)日：2023-03-16

    申请号：US17964771

    申请日：2022-10-12

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Christian Boehm ,  Amy Rowat ,  Sarah Koester ,  Jeremy Agresti

    IPC分类号： B01L3/00 ,  B01L7/00 ,  G01N33/50 ,  G01N21/64

    摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time-resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.

    Fluid injection
    8.
    发明授权
    Fluid injection 有权

    公开(公告)号：US11358105B2

    公开(公告)日：2022-06-14

    申请号：US17145684

    申请日：2021-01-11

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Adam R. Abate ,  Tony Hung ,  Pascaline Mary

    IPC分类号： B01F25/314 ,  F16K99/00 ,  B01J19/00 ,  B01L3/00 ,  B01F33/302 ,  B01F33/3031 ,  G01N27/447 ,  G01N35/10

    摘要： The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

    Coalescence of droplets
    9.
    发明授权
    Coalescence of droplets 有权

    公开(公告)号：US11141730B2

    公开(公告)日：2021-10-12

    申请号：US14896218

    申请日：2014-06-12

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Thomas E. Kodger ,  Donald Aubrecht ,  Ilke Akartuna

    IPC分类号： B01L3/00 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00

    摘要： The present invention generally relates to microfluidics, and, in particular, to systems and methods for coalescing or fusing droplets. In certain aspects, two or more droplets within a microfluidic channel are brought together and caused to coalesce without using electric fields or charges. For example, in certain embodiments, droplets stabilized with a surfactant may be disrupted, e.g., by exposing the droplets to a solvent able to alter the surfactant, which may partially destabilize the droplets and allow them to coalesce. In some instances, the droplets may also be physically disrupted to facilitate coalesce. In addition, in some cases, the positions of one or more droplets may be controlled within a channel using a groove in a wall of the channel. For example, a droplet may at least partially enter the groove such that the position of the droplet is at least partially controlled by the groove.

    DROPLET CREATION TECHNIQUES
    10.
    发明申请
    DROPLET CREATION TECHNIQUES 有权

    公开(公告)号：US20210229099A1

    公开(公告)日：2021-07-29

    申请号：US17148287

    申请日：2021-01-13

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Adam R. Abate

    IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F3/08

    摘要： The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.