公开(公告)号：US20210128790A1

公开(公告)日：2021-05-06

申请号：US17022062

申请日：2020-09-15

Applicant: President and Fellows of Harvard College

Inventor： Rajiv Desai ,  Neel Satish Joshi ,  David J. Mooney ,  Sandeep T. Koshy ,  Alexander Stafford

IPC: A61L27/52 ,  A61L27/26 ,  C07D237/26 ,  A61L27/20 ,  A61L27/22 ,  A61L27/38 ,  A61L27/54 ,  C07K14/78 ,  C08B37/00

Abstract: The present disclosure provides click-crosslinked hydrogels and methods of use.

公开(公告)号：US20190315799A1

公开(公告)日：2019-10-17

申请号：US16302315

申请日：2017-05-16

Applicant: President and Fellows of Harvard College

Inventor： Neel Satish Joshi ,  Noémie-Manuelle Dorval Courchesne ,  Anna M. Duraj-Thatte ,  Peter Q. Nguyen

IPC: C07K1/34 ,  C07K14/47 ,  C08J5/18 ,  B01D61/14 ,  D01F4/06

Abstract: Provided herein are filtration-based purification methods for amyloid fibers, such as curli fibers, directly from microbial culture, and their fabrication into free-standing thin films. Additionally, methods for recycling amyloid fibers thing films by, for example, disassembly and re-assembly, are disclosed herein.

公开(公告)号：US11278577B2

公开(公告)日：2022-03-22

申请号：US16328303

申请日：2017-08-31

Applicant: President and Fellows of Harvard College

Inventor： Peter Q. Nguyen ,  Neel Satish Joshi

IPC: C12P21/06 ,  A61K35/74 ,  C07K14/245 ,  C12P21/02 ,  A61P1/00 ,  A61K9/00 ,  A61K9/02 ,  A61K9/20 ,  A61K9/48 ,  C07K19/00

Abstract: Engineered bacteria that secrete therapeutic polypeptides, pharmaceutical compositions comprising the bacteria, methods for producing recombinant polypeptides, and methods for using the bacteria for diagnostic and therapeutic purposes are provided.

公开(公告)号：US11098133B2

公开(公告)日：2021-08-24

申请号：US16302687

申请日：2017-05-19

Applicant: President and Fellows of Harvard College

Inventor： Anna M. Duraj-Thatte ,  Noémie-Manuelle Dorval Courchesne ,  Neel Satish Joshi

IPC: C08J3/075 ,  C07K17/04 ,  A61K49/00 ,  A61K9/70 ,  C07K14/245 ,  C07K14/47 ,  C08J5/18 ,  C12N15/62 ,  B82Y30/00 ,  C12N5/00

Abstract: Provided are compositions and methods of making hydrogels, aerogels, films and composites directly from a microbial culture or complex mixture using curli nanofibers.

公开(公告)号：US10995114B2

公开(公告)日：2021-05-04

申请号：US16302315

申请日：2017-05-16

Applicant: President and Fellows of Harvard College

Inventor： Neel Satish Joshi ,  Noémie-Manuelle Dorval Courchesne ,  Anna M. Duraj-Thatte ,  Peter Q. Nguyen

IPC: C07K1/34 ,  B01D61/14 ,  C07K14/47 ,  C08J5/18 ,  D01F4/06

Abstract: Provided herein are filtration-based purification methods for amyloid fibers, such as curli fibers, directly from microbial culture, and their fabrication into free-standing thin films. Additionally, methods for recycling amyloid fibers thing films by, for example, disassembly and re-assembly, are disclosed herein.

公开(公告)号：US10821208B2

公开(公告)日：2020-11-03

申请号：US15301466

申请日：2015-04-06

Applicant: President and Fellows of Harvard College

Inventor： Rajiv Desai ,  Neel Satish Joshi ,  David J. Mooney ,  Sandeep T. Koshy ,  Alexander G. Stafford

IPC: A61L27/52 ,  C07D237/26 ,  A61L27/20 ,  A61L27/54 ,  C08B37/00 ,  A61L27/26 ,  A61L27/22 ,  A61L27/38 ,  C07K14/78

Abstract: The present disclosure provides click-crosslinked hydrogels and methods of use.

公开(公告)号：US20200255480A1

公开(公告)日：2020-08-13

申请号：US16713368

申请日：2019-12-13

Applicant: President and Fellows of Harvard College

Inventor： Neel Satish Joshi ,  Peter Q. Nguyen ,  Zsofia Magarian

IPC: C07K14/245 ,  C07K14/21

Abstract: Described herein are methods and compositions relating to engineered curli fibers, e.g. CsgA polypeptide. In some embodiments, the methods and compositions described herein relate to functionalized biofilms.

公开(公告)号：US20200248190A1

公开(公告)日：2020-08-06

申请号：US16785874

申请日：2020-02-10

Applicant: President and Fellows of Harvard College

Inventor： Neel Satish Joshi ,  Peter Q. Nguyen ,  Anna M. Duraj-Thatte ,  Pichet Praveschotinunt

IPC: C12N15/62 ,  A61K35/74 ,  C12N15/70 ,  C07K14/33 ,  A61P1/00 ,  C07K14/245

Abstract: Methods of making biofilms having non-native functional polypeptides attached thereto are provided.

公开(公告)号：US12060398B2

公开(公告)日：2024-08-13

申请号：US17254019

申请日：2019-06-21

Applicant: President and Fellows of Harvard College

Inventor： Arjirios Sourlis ,  Anna Duraj-Thatte ,  Avinash Manjula Basavanna ,  Neel Satish Joshi

IPC: C07K14/47 ,  A61L15/32 ,  A61L15/42 ,  A61L24/00 ,  A61L24/10 ,  A61L27/22 ,  A61L27/52 ,  B33Y70/00 ,  C07K14/75 ,  C07K14/78 ,  C09D11/04

CPC classification number: C07K14/4711 ,  A61L15/32 ,  A61L15/42 ,  A61L24/0031 ,  A61L24/108 ,  A61L27/227 ,  A61L27/52 ,  B33Y70/00 ,  C07K14/75 ,  C07K14/78 ,  C09D11/04 ,  C07K2319/00

Abstract: Disclosed herein are engineered bacteria that manufacture biofilms from bacterial amyloid structures. These biofilms and biofilm matrices are capable of generating fibrous proteinaceous networks and being used as 3D-printing inks.

公开(公告)号：US11643443B2

公开(公告)日：2023-05-09

申请号：US15777070

申请日：2016-11-18

Applicant: President and Fellows of Harvard College

Inventor： Neel Satish Joshi ,  Noémie-Manuelle Dorval Courchesne

IPC: C12N15/74 ,  C07K14/245 ,  C07K14/47 ,  C07K14/395 ,  C07K14/195 ,  C12N9/08 ,  C12N9/36

CPC classification number: C07K14/245 ,  C07K14/195 ,  C07K14/395 ,  C07K14/47 ,  C07K14/4711 ,  C07K14/4717 ,  C12N9/0065 ,  C12N9/2462 ,  C12Y111/01007 ,  C12Y302/01017 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/735

Abstract: Methods of making engineered protein-based materials, nanofibers, and biofilms from bacterial amyloid-based structures that are capable of mediating long-range electron transport are provided.