公开(公告)号：US20090011493A1

公开(公告)日：2009-01-08

申请号：US12166287

申请日：2008-07-01

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： C12M1/00

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

摘要翻译： 本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。 该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。

公开(公告)号：US06368838B1

公开(公告)日：2002-04-09

申请号：US09373334

申请日：1999-08-12

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： C12N1102

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： Methods and a device are provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere. The cytophobic regions can be wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. Further, the islands or the regions or both may be formed of a self-assembled monolayer (SAM). The methods are for forming a patterned surface for a population of cells and for selectively manipulating individual cells formed on the surface of the plate of the device. Furthermore, the device is used in immobilization of cells at a surface and for controlling shape of the cells.

摘要翻译： 提供了用于将细胞粘附在特定和预定位置的方法和装置。 该装置包括限定表面的板和附着由细胞不粘附细胞渗透区域分离的细胞的多个细胞色素岛。 细胞疏植区域可以足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。 此外，岛或区域或两者可以由自组装单层（SAM）形成。 这些方法用于形成用于细胞群的图案化表面并且用于选择性地操纵形成在装置的板的表面上的单个细胞。 此外，该装置用于在表面固定细胞并用于控制细胞的形状。

公开(公告)号：US5976826A

公开(公告)日：1999-11-02

申请号：US951886

申请日：1997-10-16

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： B01J19/00 ,  B81C1/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C40B60/14 ,  C12Q1/02 ,  C12N11/02 ,  C12N11/08 ,  C12Q1/04

CPC分类号： C12N5/067 ,  B01J19/0046 ,  B81C1/00206 ,  B82Y30/00 ,  B82Y5/00 ,  C12N5/0068 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C2201/034 ,  C12N2535/10 ,  C40B60/14

摘要： A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.

摘要翻译： 提供了用于将细胞粘附在特定和预定位置的装置。 该装置包括限定表面的板和多个细胞亲和岛，所述细胞亲和岛附着细胞，细胞被细胞悬浮区域分离，细胞不粘附并进一步与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。 此外，细胞疏气区域足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。 该装置用于在表面或培养基中培养细胞并用于进行细胞计数的方法。 此外，该装置用于在表面固定细胞并用于控制细胞的形状。

公开(公告)号：US5776748A

公开(公告)日：1998-07-07

申请号：US659537

申请日：1996-06-06

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： B01J19/00 ,  B81C1/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C40B60/14 ,  B32B31/00 ,  C12N11/08 ,  C12N11/14

CPC分类号： C12N5/067 ,  B01J19/0046 ,  B81C1/00206 ,  B82Y30/00 ,  B82Y5/00 ,  C12N5/0068 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C2201/034 ,  C12N2535/10 ,  C40B60/14

摘要： The disclosed invention is a device for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.

摘要翻译： 所公开的发明是用于在特定和预定位置粘附电池的装置。 该装置包括限定表面的板和多个细胞亲和岛，所述细胞亲和岛附着细胞，所述细胞由细胞不粘附的细胞渗透区域分离，并且还与细胞色素岛连续。 岛或区域或两者可以由自组装单层（SAM）形成。 此外，细胞疏气区域足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。 该装置用于在表面或培养基中培养细胞并用于进行细胞计数的方法。 此外，该装置用于在表面固定细胞并用于控制细胞的形状。

公开(公告)号：US07067306B2

公开(公告)日：2006-06-27

申请号：US10032280

申请日：2001-12-21

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： C12Q1/02 ,  C12Q1/04 ,  C12N11/02 ,  C12N11/08

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

摘要翻译： 本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。 该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。

公开(公告)号：US07993905B2

公开(公告)日：2011-08-09

申请号：US12166287

申请日：2008-07-01

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory Stephanopoulos

IPC分类号： C12N1/00 ,  C12N11/00 ,  C12N11/08

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

摘要翻译： 本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。 该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。

公开(公告)号：US07410791B2

公开(公告)日：2008-08-12

申请号：US11331849

申请日：2006-01-13

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory Stephanopoulos

IPC分类号： C12N11/00 ,  C12N11/08 ,  C12N1/00

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

摘要翻译： 本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。 该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。

公开(公告)号：US20060210595A1

公开(公告)日：2006-09-21

申请号：US11331849

申请日：2006-01-13

申请人： Rahul Singhvi ,  Amit Kumar ,  George Whitesides ,  Donald Ingber ,  Gabriel Lopez ,  Daniel Wang ,  Gregory Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George Whitesides ,  Donald Ingber ,  Gabriel Lopez ,  Daniel Wang ,  Gregory Stephanopoulos

IPC分类号： A61F2/06 ,  A61F13/00

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

公开(公告)号：US20090001049A1

公开(公告)日：2009-01-01

申请号：US12187070

申请日：2008-08-06

申请人： George M. Whitesides ,  Younan Xia ,  James L. Wilbur ,  Rebecca J. Jackman ,  Enoch Kim ,  Mara G. Prentiss ,  Milan Mrksich ,  Amit Kumar ,  Christopher B. Gorman ,  Hans Biebuyck ,  Karl K. Berggren

发明人： George M. Whitesides ,  Younan Xia ,  James L. Wilbur ,  Rebecca J. Jackman ,  Enoch Kim ,  Mara G. Prentiss ,  Milan Mrksich ,  Amit Kumar ,  Christopher B. Gorman ,  Hans Biebuyck ,  Karl K. Berggren

IPC分类号： C03C15/00

CPC分类号： B01L3/5085 ,  B01J2219/00382 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01L3/5088 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0851 ,  B05D1/283 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C40B60/14 ,  G02B5/1857 ,  G02B6/124 ,  G02B6/136 ,  G02B2006/12102 ,  G02B2006/12104 ,  G02B2006/12107 ,  G03F7/0002 ,  G03F7/165 ,  H01L21/4867 ,  H01L21/768

摘要： Improved methods of forming a patterned self-assembled monolayer on a surface and derivative articles are provided. According to one method, an elastomeric stamp is deformed during and/or prior to using the stamp to print a self-assembled molecular monolayer on a surface. According to another method, during monolayer printing the surface is contacted with a liquid that is immiscible with the molecular monolayer-forming species to effect controlled reactive spreading of the monolayer on the surface. Methods of printing self-assembled molecular monolayers on nonplanar surfaces and derivative articles are provided, as are methods of etching surfaces patterned with self-assembled monolayers, including methods of etching silicon. Optical elements including flexible diffraction gratings, mirrors, and lenses are provided, as are methods for forming optical devices and other articles using lithographic molding. A method for controlling the shape of a liquid on the surface of an article is provided, involving applying the liquid to a self-assembled monolayer on the surface, and controlling the electrical potential of the surface.

摘要翻译： 提供了在表面和衍生物品上形成图案化自组装单层的改进方法。 根据一种方法，弹性印模在使用印模期间和/或之前变形，以在表面上印刷自组装分子单层。 根据另一种方法，在单层印刷过程中，表面与与分子单层形成物质不混溶的液体接触，以实现单层在表面上的受控的反应性扩散。 提供了在非平面表面和衍生物上印刷自组装分子单层的方法，以及用自组装单层蚀刻表面的方法，包括蚀刻硅的方法。 提供包括柔性衍射光栅，反射镜和透镜的光学元件，以及用于使用光刻成型形成光学器件和其它制品的方法。 提供了一种用于控制制品表面上的液体形状的方法，包括将液体施加到表面上的自组装单层，并控制表面的电位。

公开(公告)号：US06776094B1

公开(公告)日：2004-08-17

申请号：US09164733

申请日：1998-10-01

申请人： George M. Whitesides ,  Amit Kumar

发明人： George M. Whitesides ,  Amit Kumar

IPC分类号： B41K138

CPC分类号： B01L3/5085 ,  B01J2219/00382 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01L3/5088 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0851 ,  B05D1/283 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C40B60/14 ,  G02B5/1857 ,  G02B6/124 ,  G02B6/136 ,  G02B2006/12102 ,  G02B2006/12104 ,  G02B2006/12107 ,  G03F7/0002 ,  G03F7/165 ,  H01L21/4867 ,  H01L21/768

摘要： Improved methods of forming a patterned self-assembled monolayer on a surface and derivative articles are provided. According to one method, an elastomeric stamp is deformed during and/or prior to using the stamp to print a self-assembled molecular monolayer on a surface. According to another method, during monolayer printing the surface is contacted with a liquid that is immiscible with the molecular monolayer-forming species to effect controlled reactive spreading of the monolayer on the surface. Methods of printing self-assembled molecular monolayers on nonplanar surfaces and derivative articles are provided, as are methods of etching surfaces patterned with self-assembled monolayers, including methods of etching silicon. Optical elements including flexible diffraction gratings, mirrors, and lenses are provided, as are methods for forming optical devices and other articles using lithographic molding. A method for controlling the shape of a liquid on the surface of an article is provided, involving applying the liquid to a self-assembled monolayer on the surface, and controlling the electrical potential of the surface.

摘要翻译： 提供了在表面和衍生物品上形成图案化自组装单层的改进方法。 根据一种方法，弹性印模在使用印模期间和/或之前变形，以在表面上印刷自组装分子单层。 根据另一种方法，在单层印刷过程中，表面与与分子单层形成物质不混溶的液体接触，以实现单层在表面上的受控的反应性扩散。 提供了在非平面表面和衍生物上印刷自组装分子单层的方法，以及用自组装单层蚀刻表面的方法，包括蚀刻硅的方法。 提供包括柔性衍射光栅，反射镜和透镜的光学元件，以及用于使用光刻成型形成光学器件和其它制品的方法。 提供了一种用于控制制品表面上的液体形状的方法，包括将液体施加到表面上的自组装单层，并控制表面的电位。