利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.048 秒)

    BIOSYNTHETIC ROUTES TO LONG-CHAIN ALPHA,OMEGA-HYDROXYACIDS, DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS
    1.
    发明申请
    BIOSYNTHETIC ROUTES TO LONG-CHAIN ALPHA,OMEGA-HYDROXYACIDS, DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS 有权
    长链ALPHA，欧米茄 - 羟基酸，DIACIDS及其转化为低聚物和聚合物的生物活性

    公开(公告)号：US20100285545A1

    公开(公告)日：2010-11-11

    申请号：US12436729

    申请日：2009-05-06

    申请人： Richard A. Gross ,  Wenhua Lu ,  Jon Ness ,  Jeremy Minshull

    发明人： Richard A. Gross ,  Wenhua Lu ,  Jon Ness ,  Jeremy Minshull

    IPC分类号： C12P7/64 ,  C12N1/19 ,  C12N15/74

    CPC分类号： C12P7/6427 ,  C12N9/0006 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409

    摘要： A substantially pure Candida host cell for the production of a α-carboxyl-ω-hydroxy fatty acid having a carbon chain length in the range from C6 to C22, a α,ω-dicarboxylic fatty acid having a carbon chain length in the range from C6 to C22, or mixtures thereof, is provided. The Candida host cell is characterized by a first genetic modification class and a second genetic modification class. The first genetic modification class comprises one or more genetic modifications that disrupt the peroxisomal β-oxidation pathway. The second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt at least one gene selected from the group consisting of a CYP52A type cytochrome P450, a fatty alcohol oxidase, and an alcohol dehydrogenase.

    摘要翻译： 用于生产碳链长度在C6至C22范围内的α-羧基-ω-羟基脂肪酸的基本上纯的假丝酵母宿主细胞，具有碳链长度范围的α，ω-二羧酸脂肪酸 C6至C22，或其混合物。 念珠菌宿主细胞的特征在于第一遗传修饰类和第二遗传修饰类。 第一种遗传修饰类包括一种或多种破坏过氧化物酶体和氧化途径的遗传修饰。 第二遗传修饰类包括共同或单独破坏选自CYP52A型细胞色素P450，脂肪醇氧化酶和醇脱氢酶中的至少一种基因的一种或多种遗传修饰。

    Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
    2.
    发明授权
    Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain 有权
    使用遗传修饰的假丝酵母菌株生产α-羧基-ω-羟基脂肪酸

    公开(公告)号：US08158391B2

    公开(公告)日：2012-04-17

    申请号：US12436729

    申请日：2009-05-06

    申请人： Richard A. Gross ,  Wenhua Lu ,  Jon Ness ,  Jeremy Minshull

    发明人： Richard A. Gross ,  Wenhua Lu ,  Jon Ness ,  Jeremy Minshull

    IPC分类号： C12P7/64 ,  C12N9/00 ,  C12N1/00 ,  C12N15/00 ,  C12N15/74

    CPC分类号： C12P7/6427 ,  C12N9/0006 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409

    摘要： A substantially pure Candida host cell for the production of a α-carboxyl-ω-hydroxy fatty acid having a carbon chain length in the range from C6 to C22, a α,ω-dicarboxylic fatty acid having a carbon chain length in the range from C6 to C22, or mixtures thereof, is provided. The Candida host cell is characterized by a first genetic modification class and a second genetic modification class. The first genetic modification class comprises one or more genetic modifications that disrupt the peroxisomal β-oxidation pathway. The second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt at least one gene selected from the group consisting of a CYP52A type cytochrome P450, a fatty alcohol oxidase, and an alcohol dehydrogenase.

    摘要翻译： 用于生产碳链长度在C6至C22范围内的α-羧基-ω-羟基脂肪酸的基本上纯的假丝酵母宿主细胞，具有碳链长度范围的α，ω-二羧酸脂肪酸 C6至C22，或其混合物。 念珠菌宿主细胞的特征在于第一遗传修饰类和第二遗传修饰类。 第一种遗传修饰类包括一种或多种破坏过氧化物酶体和氧化途径的遗传修饰。 第二遗传修饰类包括共同或单独破坏选自CYP52A型细胞色素P450，脂肪醇氧化酶和醇脱氢酶中的至少一种基因的一种或多种遗传修饰。

    Systems and methods for designing and ordering polynucleotides
    3.
    发明申请
    Systems and methods for designing and ordering polynucleotides 有权
    用于设计和排序多核苷酸的系统和方法

    公开(公告)号：US20070043516A1

    公开(公告)日：2007-02-22

    申请号：US11207151

    申请日：2005-08-16

    申请人： Claes Gustafsson ,  Sridhar Govindarajan ,  Jon Ness ,  Alan Villalobos ,  Jeremy Minshull

    发明人： Claes Gustafsson ,  Sridhar Govindarajan ,  Jon Ness ,  Alan Villalobos ,  Jeremy Minshull

    IPC分类号： G06F19/00

    CPC分类号： G06F19/26 ,  G06F19/18 ,  G06F19/20

    摘要： Computer systems, computer program products and methods for designing oligonucleotides are provided. A set of sequence elements is defined. Each sequence element represents an amino acid sequence segment or a nucleic acid sequence segment. The set of sequence elements collectively represent a design nucleic acid sequence. The set of sequence elements are displayed as a plurality icons in a linear or a near linear arrangement such that each respective icon in the plurality of icons uniquely represents a corresponding sequence element in the set of sequence elements. In this representation, neighboring icons in the plurality of icons represent neighboring sequence elements in the set of sequence elements. Each respective icon in the plurality of icons depicts a directional property for the corresponding sequence element in the set of sequence elements. An oligonucleotide selection module is used to identify oligonucleotides in the design nucleic acid sequence.

    摘要翻译： 提供计算机系统，计算机程序产品和设计寡核苷酸的方法。 定义了一组序列元素。 每个序列元件代表氨基酸序列片段或核酸序列片段。 序列元件的集合统称为设计核酸序列。 序列元素的集合被显示为线性或近似线性布置的多个图标，使得多个图标中的每个相应图标唯一地表示序列元素集合中的相应序列元素。 在该表示中，多个图标中的相邻图标表示该组序列元素中的相邻序列元素。 多个图标中的每个相应的图标描绘了序列元素集合中相应的序列元素的方向属性。 寡核苷酸选择模块用于鉴定设计核酸序列中的寡核苷酸。

    Use of codon-varied oligonucleotide synthesis for synthetic shuffling
    8.
    发明授权
    Use of codon-varied oligonucleotide synthesis for synthetic shuffling 失效
    使用密码子变化的寡核苷酸合成进行合成洗牌

    公开(公告)号：US06436675B1

    公开(公告)日：2002-08-20

    申请号：US09408393

    申请日：1999-09-28

    申请人： Mark Welch ,  Jon Ness ,  Claes Gustafsson ,  Willem P. C. Stemmer ,  Jeremy Minshull

    发明人： Mark Welch ,  Jon Ness ,  Claes Gustafsson ,  Willem P. C. Stemmer ,  Jeremy Minshull

    IPC分类号： C12Q168

    CPC分类号： C12N15/1031 ,  B01J2219/00689 ,  C12N15/11

    摘要： Methods of providing shuffling libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.

    摘要翻译： 描述了提供包括密码子变化的寡核苷酸序列的改组文库的方法。 使用三核苷酸或单核苷酸亚磷酰胺序列合成密码子多样的寡核苷酸，并衍生自同源或非同源核酸序列或这些序列的组合。 还描述了重组密码子变化的寡核苷酸序列以加速人造进化的各种方法。 本发明还涉及可选地用于使这些重组方法自动化的各种集成系统。