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    Antibody diversity generation
    8.
    发明申请
    Antibody diversity generation 审中-公开
    抗体多样性生成

    公开(公告)号：US20050038232A1

    公开(公告)日：2005-02-17

    申请号：US10686945

    申请日：2003-10-16

    申请人： Erik Karrer ,  Steven Bass ,  Robert Whalen ,  Phillip Patten

    发明人： Erik Karrer ,  Steven Bass ,  Robert Whalen ,  Phillip Patten

    IPC分类号： C07K16/00 ,  C07K16/12 ,  C12N1/21 ,  C12N15/13 ,  C12N15/49 ,  C07K16/46 ,  C07H21/04

    CPC分类号： C07K16/1271 ,  C07K16/00 ,  C07K16/1275 ,  C07K2317/24

    摘要： Methods for improving antibodies by a variety of DNA diversification and selection procedures are provided. Improvements include increases in affinity, alterations in specificity and effector function, as well as reduced antigenicity, e.g. humanization. Libraries of recombinant antibody sequences are provided, as are cells expressing members of such libraries. Novel phage display vectors are provided. Methods for the coevolution of an antibody and its cognate antigen are provided. Coevolution is used to evolve HIV envelope proteins with increased antigenicity and broadly neutralizing antibodies that interact therewith. Methods of improving antibodies for use in the detection of biological warfare agents are provided.

    摘要翻译： 提供了通过多种DNA多样化和选择程序改进抗体的方法。 改善包括亲和力的增加，特异性和效应子功能的改变，以及降低的抗原性，例如。 人性化。 提供重组抗体序列的文库，以及表达这些文库成员的细胞。 提供了新的噬菌体展示载体。 提供了抗体及其同源抗原共转化方法。 共转化用于发展具有增加的抗原性的HIV包膜蛋白和与之相互作用的广泛中和抗体。 提供了改进用于检测生物战剂的抗体的方法。

    Method of controlling polypeptide production in bacterial cells
    9.
    发明授权
    Method of controlling polypeptide production in bacterial cells 失效
    控制细菌细胞中多肽生成的方法

    公开(公告)号：US5304472A

    公开(公告)日：1994-04-19

    申请号：US989845

    申请日：1992-11-20

    申请人： Steven Bass ,  James R. Swartz

    发明人： Steven Bass ,  James R. Swartz

    IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/245 ,  C07K14/65 ,  C12N1/21 ,  C12N1/38 ,  C12N15/12 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19 ,  C12N1/00 ,  C12N15/08 ,  C12N15/31 ,  C12N15/67

    CPC分类号： C07K14/245 ,  C07K14/65 ,  C12N1/38 ,  C12N15/70 ,  A61K39/00

    摘要： Nucleic acid is provided encoding a molecule having certain variations within the phosphate-binding region of native E. coli PstS. Additionally provided are bacterial cells comprising this nucleic acid under control of the native pstS gene promoter, and optionally further comprising nucleic acid encoding a polypeptide of interest under control of the alkaline phosphatase (AP) promoter. Bacterial cells containing both pstS variant nucleic acid and polypeptide nucleic acid are cultured in a medium at a concentration of inorganic phosphate that at all phases of cell growth is above the level at which the cells are starved for phosphate. Alternatively, the cells are cultured under conditions whereby the concentration of inorganic phosphate in the culture medium is controlled during the production period so that the polypeptide is produced under the control of the partially induced AP promoter.

    摘要翻译： 提供编码在天然大肠杆菌PstS的磷酸结合区域内具有某些变异的分子的核酸。 另外提供了包含在天然pstS基因启动子控制下的该核酸的细菌细胞，以及任选地还包含编码在碱性磷酸酶（AP）启动子控制下的感兴趣多肽的核酸。 含有pstS变体核酸和多肽核酸两者的细菌细胞在无机磷酸盐的浓度的培养基中培养，在细胞生长的所有阶段都高于细胞饥饿磷酸盐的水平。 或者，在生产期间控制培养基中的无机磷酸盐浓度的条件下培养细胞，使得多肽在部分诱导的AP启动子的控制下产生。