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    Plant acyl-ACP thioesterase sequences
    9.
    发明授权
    Plant acyl-ACP thioesterase sequences 失效
    植物酰基-ACP硫酯酶序列

    公开(公告)号：US5723761A

    公开(公告)日：1998-03-03

    申请号：US464523

    申请日：1995-06-02

    申请人： Toni A. Voelker ,  Ling Yuan ,  Jean Kridl ,  Deborah Hawkins ,  Aubrey Jones

    发明人： Toni A. Voelker ,  Ling Yuan ,  Jean Kridl ,  Deborah Hawkins ,  Aubrey Jones

    IPC分类号： A01H5/00 ,  A23D9/00 ,  C11C3/00 ,  C12N5/10 ,  C12N9/16 ,  C12N15/09 ,  C12N15/55 ,  C12N15/82 ,  A01H5/10 ,  C12N5/14 ,  C12N15/29 ,  C12N15/52

    CPC分类号： A23D9/00 ,  C12N15/8247 ,  C12N9/16

    摘要： This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.

    摘要翻译： 本发明涉及植物硫酯酶，特别是具有对棕榈酰-ACP底物具有显着活性的植物酰基-ACP硫酯酶。 描述了可用于在植物种子细胞中表达植物棕榈酰-ACP硫酯酶的DNA构建体。 与其他植物组织相比，这样的构建体将含有编码植物棕榈酰-ACP硫酯酶的DNA序列，该调控元件能够优先指导植物棕榈酰-ACP硫酯酶在种子组织中的表达， 在转基因植物中表达。 本发明还涉及使用编码植物棕榈酰-ACP硫酯酶的DNA序列来修饰植物种子细胞中产生的游离脂肪酸的比例的方法。 本文例示的植物棕榈酰-ACP硫酯酶序列包括Cuphea，韭葱，芒果和榆树。 还提供了作为这些棕榈酰-ACP硫酯酶序列表达的结果，在其种子中具有增加的C16：0脂肪酸水平的转基因植物。

    Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
    10.
    发明授权
    Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity 失效
    工程植物硫酯酶和具有新颖底物特异性的植物硫酯酶的公开

    公开(公告)号：US6150512A

    公开(公告)日：2000-11-21

    申请号：US868458

    申请日：1997-06-03

    申请人： Ling Yuan

    发明人： Ling Yuan

    IPC分类号： C12N15/09 ,  C12N9/16 ,  C12N15/29 ,  C12N15/55 ,  C12N15/82 ,  C12N15/52

    CPC分类号： C12N9/16 ,  C12N15/8247

    摘要： Methods of altering substrate specificity of plant acyl-ACP thioesterases, and engineered plant acyl-ACP thioesterases so produced are provided. The C-terminal two thirds portion of plant thioesterases is identified as desirable for such modifications.DNA sequences and constructs for expression of engineered thioesterases, as well as the novel thioesterases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered thioesterases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition. Of particular interest is a mangosteen Garm FatA1 18:1 thioesterase in which the relative 18:0 activity has been increased. Such FatA thioesterases find use for improved production of stearate in vegetable seed oils.

    摘要翻译： 提供了改变植物酰基-ACP硫酯酶底物特异性的方法，以及如此制备的工程化植物酰基-ACP硫酯酶。 植物硫酯酶的C末端三分之二部分被鉴定为对于这种修饰是理想的。 还提供了用于表达工程化硫酯酶的DNA序列和构建体，以及由其产生的新的硫酯酶。 这样的DNA序列可用于在宿主细胞，特别是油籽作物植物的种子细胞中表达改性的硫酯酶，用于改变脂肪酸组成。 特别令人感兴趣的是山甘菊Garm FatA1 18：1硫酯酶，其中相对18：0的活性已经增加。 这种FatA硫酯酶可用于改善植物油中硬脂酸酯的生产。