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公开(公告)号：US06953663B1
公开(公告)日：2005-10-11
申请号：US10607829
申请日：2003-06-27
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记（例如杂合子或两个纯合子中的任一个）的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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公开(公告)号：US06586186B2
公开(公告)日：2003-07-01
申请号：US09939119
申请日：2001-08-24
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00695 , B01J2219/00722 , C07H21/04 , C12Q1/6837 , C40B40/06
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
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公开(公告)号：US20120258879A1
公开(公告)日：2012-10-11
申请号：US13297072
申请日：2011-11-15
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G16B25/00 , G16B30/00 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析
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公开(公告)号：US06300063B1
公开(公告)日：2001-10-09
申请号：US08853370
申请日：1997-05-08
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00695 , B01J2219/00722 , C07H21/04 , C12Q1/6837 , C40B40/06
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记（例如杂合子或两个纯合子中的任一个）的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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公开(公告)号：US08095323B2
公开(公告)日：2012-01-10
申请号：US12692729
申请日：2010-01-25
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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公开(公告)号：US07674587B2
公开(公告)日：2010-03-09
申请号：US11675980
申请日：2007-02-16
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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7.发明申请公开(公告)号：US20120329677A9
公开(公告)日：2012-12-27
申请号：US11506176
申请日：2006-08-18
申请人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
CPC分类号： C12Q1/6874 , C12Q1/6886
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.
摘要翻译： 本发明提供了固定化探针的阵列，以及采用阵列的方法，用于检测CFTR基因中的突变。
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8.发明申请Capturing sequences adjacent to type IIs restriction sites for genomic library mapping 审中-公开捕获与II型限制性位点相邻的序列用于基因组文库作图公开(公告)号：US20120258868A1
公开(公告)日：2012-10-11
申请号：US13159626
申请日：2011-06-14
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致捕获和确定位于II型限制性位点附近的特异性寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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公开(公告)号：US20120172246A1
公开(公告)日：2012-07-05
申请号：US13342392
申请日：2012-01-03
申请人： Quan N. Nguyen , Robert J. Lipshutz , Gary K. McMaster , Yunqing Ma , Yen-Chieh Wu , Audrey Lin , Chunfai Lai , Cung-Tuong Nguyen
发明人： Quan N. Nguyen , Robert J. Lipshutz , Gary K. McMaster , Yunqing Ma , Yen-Chieh Wu , Audrey Lin , Chunfai Lai , Cung-Tuong Nguyen
CPC分类号： C12Q1/682 , C12Q2525/113 , C12Q2525/161 , C12Q2537/125 , C12Q2537/143
摘要： Methods of detecting various types of nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Detection assays may be conducted at least in vitro, in cellulo, and in situ. Nucleic acids which are optionally captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label probe system with the nucleic acids. Various label probe system embodiments are provided. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供检测各种类型核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测试验可以至少在体外，纤维素和原位进行。 可以例如通过导致标记探针系统与核酸的特异性缔合的协同杂交事件来检测任选地捕获在固体支持物上的核酸。 提供了各种标签探针系统实施例。 还描述了与方法相关的组合物，试剂盒和系统。
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10.发明申请Capturing Sequences Adjacent to type-IIs restriction sites for Genomic Library Mapping 失效捕获序列与基因组图谱绘制的II型限制性位点相邻公开(公告)号：US20100261616A1
公开(公告)日：2010-10-14
申请号：US12272680
申请日：2008-11-17
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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