公开(公告)号：US08524891B2

公开(公告)日：2013-09-03

申请号：US11995244

申请日：2006-07-14

申请人： Robert P. Hammer ,  Steven A. Soper ,  Serhii Pakhomov ,  Timothy J. Jensen ,  Michael W. Allen ,  Irina V. Nesterova ,  Maria da Graça Henriques Vicente

发明人： Robert P. Hammer ,  Steven A. Soper ,  Serhii Pakhomov ,  Timothy J. Jensen ,  Michael W. Allen ,  Irina V. Nesterova ,  Maria da Graça Henriques Vicente

IPC分类号： C07D487/22 ,  C07B47/00

CPC分类号： C07D487/22

摘要： Asymmetrically substituted metal-phthalocyanine compounds are disclosed. These compounds and other phthalocyanine-derivatives are used in bioimaging, bioanalysis, FRET and quenching techniques, photodynamic therapy, DNA analysis for cells, proteins, tissues and other biological entities, and other applications. Near-infrared fluorescence minimizes matrix effects typically seen in other methods of analyzing biochemical entities in cells, proteins, tissues and other biological entities.

摘要翻译： 公开了不对称取代的金属酞菁化合物。 这些化合物和其他酞菁衍生物用于生物成像，生物分析，FRET和淬灭技术，光动力学治疗，细胞，蛋白质，组织和其他生物实体的DNA分析以及其他应用。 近红外荧光最小化通常在分析细胞，蛋白质，组织和其他生物实体中的生物化学实体的其他方法中看到的基质效应。

公开(公告)号：US20090215105A1

公开(公告)日：2009-08-27

申请号：US11995244

申请日：2006-07-14

申请人： Robert P. Hammer ,  Steven a. Soper ,  Serhii Pakhomov ,  Timothy J. Jensen ,  Michael W. Allen ,  Irina v. Nesterova ,  Maria da Graca Henriques Vicente

发明人： Robert P. Hammer ,  Steven a. Soper ,  Serhii Pakhomov ,  Timothy J. Jensen ,  Michael W. Allen ,  Irina v. Nesterova ,  Maria da Graca Henriques Vicente

IPC分类号： C12Q1/02 ,  C07D487/22 ,  C12P19/34

CPC分类号： C07D487/22

摘要： Asymmetrically substituted metal-phthalocyanine compounds are disclosed. These compounds and other phthalo-cyanine-derivatives are used in bioimaging, bioanalysis, FRET and quenching techniques, photodynamic therapy, DNA analysis for cells, proteins, tissues and other biological entities, and other applications. Near-infrared fluorescence minimizes matrix effects typically seen in other methods of analyzing biochemical entities in cells, proteins, tissues and other biological entities.

摘要翻译： 公开了不对称取代的金属酞菁化合物。 这些化合物和其他酞菁衍生物用于生物成像，生物分析，FRET和猝灭技术，光动力学治疗，细胞，蛋白质，组织和其他生物实体的DNA分析和其他应用。 近红外荧光最小化通常在分析细胞，蛋白质，组织和其他生物实体中的生物化学实体的其他方法中看到的基质效应。

公开(公告)号：US09221877B2

公开(公告)日：2015-12-29

申请号：US13119549

申请日：2009-09-16

申请人： William Hansel ,  Sita Aggarwal ,  Robert P. Hammer

发明人： William Hansel ,  Sita Aggarwal ,  Robert P. Hammer

IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K7/23

CPC分类号： A61K47/64 ,  A61K38/00 ,  A61K38/24 ,  A61K47/54 ,  C07K7/23

摘要： Conjugating LHRH to curcumin (LHRH-Curcumin) substantially enhances the bioavailability of curcumin, targets it to cells expressing LHRH receptors, facilitates intravenous administration, and increases the anti-cancer efficacy of curcumin. The conjugate may be used against cancer cells that express the LHRH receptor: pancreas, prostate, breast, testicular, uterine, ovarian, melanoma. LH-Curcumin conjugates may be used against cancer cells that express the LH receptor: prostate, breast, ovary, testis, uterus, pancreas, and melanoma.

摘要翻译： 将LHRH与姜黄素（LHRH-姜黄素）结合显着提高姜黄素的生物利用度，将其靶向表达LHRH受体的细胞，促进静脉内施用，并增加姜黄素的抗癌功效。 该缀合物可用于表达LHRH受体的癌细胞：胰腺，前列腺，乳腺，睾丸，子宫，卵巢，黑素瘤。 LH-姜黄素结合物可用于表达LH受体的癌细胞：前列腺，乳腺，卵巢，睾丸，子宫，胰腺和黑素瘤。

公开(公告)号：US07914981B2

公开(公告)日：2011-03-29

申请号：US10854678

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US08703928B2

公开(公告)日：2014-04-22

申请号：US13229198

申请日：2011-09-09

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07892747B2

公开(公告)日：2011-02-22

申请号：US12725248

申请日：2010-03-16

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07892746B2

公开(公告)日：2011-02-22

申请号：US12725180

申请日：2010-03-16

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07262163B2

公开(公告)日：2007-08-28

申请号：US10414342

申请日：2003-04-14

申请人： Mark L. McLaughlin ,  Thomas S. Yokum ,  Frederick M. Enright ,  Philip H. Elzer ,  Robert P. Hammer

发明人： Mark L. McLaughlin ,  Thomas S. Yokum ,  Frederick M. Enright ,  Philip H. Elzer ,  Robert P. Hammer

IPC分类号： A61K38/03 ,  C07K4/00

CPC分类号： C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  Y02A50/402 ,  Y02A50/481 ,  Y10S977/903

摘要： “Minimalist” antimicrobial peptides are disclosed based on 50 to 80% α,α-dialkylated amino acids. The peptides are short, cationic, amphipathic, and possess a high helix propensity. Polar α,α-dialkylated amino acids are also disclosed. These peptides are easy and inexpensive to synthesize via solid-phase techniques. The peptides exhibit in vitro anti-bacterial properties at concentrations that are not lethal to normal mammalian cells. The peptides exhibit in vivo bioactivity against intracellular pathogens.

摘要翻译： 基于50至80％的α，α-二烷基化氨基酸公开了“极简”抗微生物肽。 肽短，阳离子，两亲，具有高螺旋倾向。 还公开了极性α，α-二烷基化氨基酸。 这些肽通过固相技术合成是容易和便宜的。 这些肽在不对正常哺乳动物细胞致死的浓度下表现出体外抗细菌特性。 肽表现出体内对细胞内病原体的生物活性。

公开(公告)号：US07166434B2

公开(公告)日：2007-01-23

申请号：US10843720

申请日：2004-05-12

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

摘要翻译： 本发明涉及使用偶联连接酶检测反应和聚合酶链反应检测核酸序列差异。 本发明的一个方面涉及使用与聚合酶链式反应偶联的连接酶检测反应。 本发明的另一方面涉及与连接酶检测反应偶联的二级聚合酶链反应偶联的初级聚合酶链式反应的用途。 本发明的第三方面涉及与第二聚合酶链反应偶联的初级聚合酶链反应。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

公开(公告)号：US07097980B2

公开(公告)日：2006-08-29

申请号：US10852289

申请日：2004-05-24

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer ,  Phillip Belgrader

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer ,  Phillip Belgrader

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.