公开(公告)号：US20180320229A1

公开(公告)日：2018-11-08

申请号：US16034392

申请日：2018-07-13

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Daniel Burgess ,  Heidi J. Rosenbaum ,  Stacey Stanislaw

IPC分类号： C12Q1/6874

CPC分类号： C12Q1/6874 ,  C12Q1/6869

摘要： In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

公开(公告)号：US20230250476A1

公开(公告)日：2023-08-10

申请号：US18131577

申请日：2023-04-06

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Daniel Burgess ,  Heidi J. Rosenbaum ,  Stacey Stanislaw

IPC分类号： C12Q1/6874 ,  C12Q1/6869

CPC分类号： C12Q1/6874 ,  C12Q1/6869

摘要： In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

公开(公告)号：US11603565B2

公开(公告)日：2023-03-14

申请号：US17252222

申请日：2019-06-14

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Aoune Barhoumi ,  Jan Berka ,  Rui Chen ,  Lisa L. Gallegos ,  Toumy Guettouche ,  Seoyoung Kim ,  Maeve E. O'Huallachain ,  Sedide Ozturk ,  Jigar Patel ,  Florian Rubelt ,  Stacey Stanislaw

IPC分类号： C12Q1/6883 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6881

摘要： The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

公开(公告)号：US20210285049A1

公开(公告)日：2021-09-16

申请号：US17252222

申请日：2019-06-14

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Aoune Barhoumi ,  Jan Berka ,  Rui Chen ,  Lisa L. Gallegos ,  Toumy Guettouche ,  Seoyoung Kim ,  Maeve E. O'Huallachain ,  Sedide Ozturk ,  Jigar Patel ,  Florian Rubelt ,  Stacey Stanislaw

IPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6881

摘要： The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

公开(公告)号：US11649492B2

公开(公告)日：2023-05-16

申请号：US16034392

申请日：2018-07-13

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R Alexander ,  Daniel Burgess ,  Heidi J Rosenbaum ,  Stacey Stanislaw

IPC分类号： C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6874 ,  C12Q1/6869

摘要： In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

公开(公告)号：US20240210288A1

公开(公告)日：2024-06-27

申请号：US18599067

申请日：2024-03-07

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  Aoune Barhoumi ,  Melinda Day ,  Lisa Gallegos ,  Katherine Leith ,  Samantha Rajkovich ,  Esteban Roberts ,  Stacey Stanislaw ,  Eric Walk

IPC分类号： G01N1/28 ,  A01N1/02 ,  G01N1/30 ,  G01N1/36 ,  G01N33/483

CPC分类号： G01N1/286 ,  A01N1/0226 ,  A01N1/0278 ,  G01N1/30 ,  G01N1/36 ,  G01N33/4833 ,  G01N2001/2866 ,  G01N2001/368

摘要： The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially “personalized” anti-tumor vaccines or immune cell based therapies.

公开(公告)号：US20200319193A1

公开(公告)日：2020-10-08

申请号：US16856619

申请日：2020-04-23

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： G01N33/58 ,  C12Q1/682 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US20180328932A1

公开(公告)日：2018-11-15

申请号：US16038389

申请日：2018-07-18

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： G01N33/58 ,  G01N33/542 ,  C12Q1/68 ,  G01N33/53 ,  C12Q1/682

CPC分类号： G01N33/581 ,  C12Q1/68 ,  C12Q1/682 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US11906523B2

公开(公告)日：2024-02-20

申请号：US16856589

申请日：2020-04-23

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： C07H21/02 ,  G01N33/58 ,  C12Q1/682 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542

CPC分类号： G01N33/581 ,  C12Q1/68 ,  C12Q1/682 ,  G01N33/53 ,  G01N33/542 ,  C07H21/02 ,  C07K14/00

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US20200326345A1

公开(公告)日：2020-10-15

申请号：US16786647

申请日：2020-02-10

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： G01N33/58 ,  C12Q1/682 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.