公开(公告)号：US11603565B2

公开(公告)日：2023-03-14

申请号：US17252222

申请日：2019-06-14

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Aoune Barhoumi ,  Jan Berka ,  Rui Chen ,  Lisa L. Gallegos ,  Toumy Guettouche ,  Seoyoung Kim ,  Maeve E. O'Huallachain ,  Sedide Ozturk ,  Jigar Patel ,  Florian Rubelt ,  Stacey Stanislaw

IPC分类号： C12Q1/6883 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6881

摘要： The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

公开(公告)号：US20210285049A1

公开(公告)日：2021-09-16

申请号：US17252222

申请日：2019-06-14

申请人： Roche Sequencing Solutions, Inc. ,  Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Aoune Barhoumi ,  Jan Berka ,  Rui Chen ,  Lisa L. Gallegos ,  Toumy Guettouche ,  Seoyoung Kim ,  Maeve E. O'Huallachain ,  Sedide Ozturk ,  Jigar Patel ,  Florian Rubelt ,  Stacey Stanislaw

IPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6881

摘要： The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

公开(公告)号：US11959838B2

公开(公告)日：2024-04-16

申请号：US15965786

申请日：2018-04-27

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  Aoune Barhoumi ,  Melinda Day ,  Lisa Gallegos ,  Katherine Leith ,  Samantha Rajkovich ,  Esteban Roberts ,  Stacey Stanislaw ,  Eric Walk

IPC分类号： G01N1/28 ,  A01N1/02 ,  G01N1/30 ,  G01N1/36 ,  G01N33/483

CPC分类号： G01N1/286 ,  A01N1/0226 ,  A01N1/0278 ,  G01N1/30 ,  G01N1/36 ,  G01N33/4833 ,  G01N2001/2866 ,  G01N2001/368

摘要： The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially “personalized” anti-tumor vaccines or immune cell based therapies.

公开(公告)号：US20240210288A1

公开(公告)日：2024-06-27

申请号：US18599067

申请日：2024-03-07

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  Aoune Barhoumi ,  Melinda Day ,  Lisa Gallegos ,  Katherine Leith ,  Samantha Rajkovich ,  Esteban Roberts ,  Stacey Stanislaw ,  Eric Walk

IPC分类号： G01N1/28 ,  A01N1/02 ,  G01N1/30 ,  G01N1/36 ,  G01N33/483

CPC分类号： G01N1/286 ,  A01N1/0226 ,  A01N1/0278 ,  G01N1/30 ,  G01N1/36 ,  G01N33/4833 ,  G01N2001/2866 ,  G01N2001/368

摘要： The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially “personalized” anti-tumor vaccines or immune cell based therapies.

公开(公告)号：US20230174585A1

公开(公告)日：2023-06-08

申请号：US18103381

申请日：2023-01-30

申请人： Ventana Medical Systems, Inc.

发明人： Rui Hong ,  Aoune Barhoumi

IPC分类号： C07K14/00 ,  C07H21/00

CPC分类号： C07K14/003 ,  C07H21/00 ,  C12N2310/3181

摘要： The present disclosure is directed to conjugates of a specific binding entity and an oligomer, i.e. [Specific Binding Entity]-[Oligomer]n, wherein n is an integer ranging from 1 to 12, and where the Oligomer includes, in some embodiments, a PNA sequence having at least one substituent at a gamma carbon position. In some embodiments, the substituent at the gamma carbon position, e.g. an amino acid, a peptide, a miniPEG, or a polymer, includes at least one reporter moiety.

公开(公告)号：US11597925B2

公开(公告)日：2023-03-07

申请号：US16438088

申请日：2019-06-11

申请人： Ventana Medical Systems, Inc.

发明人： Aoune Barhoumi ,  Rui Hong

IPC分类号： C07H21/04 ,  C12N15/11 ,  C07K2/00 ,  C07K14/00 ,  C07K16/28 ,  G01N33/53 ,  G01N33/58 ,  G01N33/68

摘要： The disclosure is directed to conjugates, e.g. PNA conjugates, as well as methods of employing the conjugates for detecting one or more targets in a biological sample, e.g. a tissue sample.

公开(公告)号：US20240077391A1

公开(公告)日：2024-03-07

申请号：US18448360

申请日：2023-08-11

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  Aoune Barhoumi ,  Lisa Gallegos ,  Stacey Stanislaw

IPC分类号： G01N1/40 ,  B01L3/00 ,  C12Q1/6886 ,  G01N1/28 ,  G01N33/574

CPC分类号： G01N1/4077 ,  B01L3/502753 ,  B01L3/502761 ,  C12Q1/6886 ,  G01N1/286 ,  G01N33/57496 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2400/0403 ,  B01L2400/086 ,  G01N2001/2866 ,  G01N2001/4088

摘要： The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.

公开(公告)号：US20230151361A1

公开(公告)日：2023-05-18

申请号：US17966904

申请日：2022-10-17

申请人： Ventana Medical Systems, Inc.

发明人： Aoune Barhoumi ,  Rui Hong

IPC分类号： C12N15/11 ,  C07K2/00 ,  C07K14/00 ,  C07K16/28 ,  G01N33/53 ,  G01N33/58 ,  G01N33/68

CPC分类号： C12N15/111 ,  C07K2/00 ,  C07K14/003 ,  C07K16/28 ,  G01N33/5308 ,  G01N33/582 ,  G01N33/6857 ,  C12N2310/113 ,  C12N2310/3511 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2310/52

摘要： The disclosure is directed to conjugates, e.g. PNA conjugates, as well as methods of employing the conjugates for detecting one or more targets in a biological sample, e.g. a tissue sample.

公开(公告)号：US20210270837A1

公开(公告)日：2021-09-02

申请号：US17203832

申请日：2021-03-17

申请人： Ventana Medical Systems, Inc.

发明人： Nelson R. Alexander ,  Aoune Barhoumi ,  Lisa L. Gallegos

IPC分类号： G01N33/574 ,  G01N15/14

摘要： Disclosed herein is a method of analyzing flow cytometry data for cells derived from homogenized whole tumor samples.

公开(公告)号：US20200339633A1

公开(公告)日：2020-10-29

申请号：US16889495

申请日：2020-06-01

申请人： Ventana Medical Systems, Inc.

发明人： Rui Hong ,  Aoune Barhoumi

IPC分类号： C07K14/00 ,  C07H21/00

摘要： The present disclosure is directed to conjugates of a specific binding entity and an oligomer, i.e. [Specific Binding Entity]-[Oligomer]n, wherein n is an integer ranging from 1 to 12, and where the Oligomer includes, in some embodiments, a PNA sequence having at least one substituent at a gamma carbon position. In some embodiments, the substituent at the gamma carbon position, e.g. an amino acid, a peptide, a miniPEG, or a polymer, includes at least one reporter moiety.