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    Engineered microorganism producing homo-succinic acid and method for preparing succinic acid using the same
    1.
    发明授权
    Engineered microorganism producing homo-succinic acid and method for preparing succinic acid using the same 有权
    产生同型琥珀酸的工程微生物及使用其制备琥珀酸的方法

    公开(公告)号：US09217138B2

    公开(公告)日：2015-12-22

    申请号：US12359322

    申请日：2009-01-25

    申请人： Sang Yup Lee ,  Sung Won Lim ,  Hyohak Song

    发明人： Sang Yup Lee ,  Sung Won Lim ,  Hyohak Song

    IPC分类号： C12P7/46 ,  C12N9/12 ,  C12N1/20 ,  C12N15/90 ,  C12N9/04 ,  C12N9/10

    CPC分类号： C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

    摘要： The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.

    摘要翻译： 本发明涉及一种突变体微生物，其选自人嗜血杆菌属（Mannheimia），放线杆菌属（Actinobacillus）和放线虫属（Anaerobiospirillum）属，产生同型琥珀酸，以及使用其制备均琥珀酸的方法，更具体地说，涉及突变体 通过破坏编码乳酸脱氢酶（ldhA）的基因，编码磷酸转乙酰酶（pta）的基因和编码乙酸激酶（ackA）的基因而获得的在厌氧条件下产生很少或不存在其它有机酸的微生物，产生高浓度的琥珀酸 ），而不破坏编码丙酮酸甲酸裂合酶（pfl）的基因，以及使用其生产琥珀酸的方法。 与生产琥珀酸的现有菌株相比，本发明的突变微生物具有生长速度快和琥珀酸生产率少的特性，同时产生很少或不含有机酸。 因此，本发明的突变微生物可用于生产用于工业用途的琥珀酸。

    Gene encoding formate dehydrogenases D & E and method for preparing succinic acid using the same
    2.
    发明授权
    Gene encoding formate dehydrogenases D & E and method for preparing succinic acid using the same 有权
    编码甲酸脱氢酶D＆E的基因及使用其制备琥珀酸的方法

    公开(公告)号：US07241594B2

    公开(公告)日：2007-07-10

    申请号：US11228945

    申请日：2005-09-16

    申请人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

    发明人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

    IPC分类号： C12P1/00 ,  C12P21/04

    CPC分类号： C12P7/46 ,  C12N9/0008

    摘要： Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.

    摘要翻译： 编码甲酸脱氢酶D＆E的核苷酸序列和使用其编码琥珀酸的方法，更具体地说，将甲酸转化为二氧化碳和氢的甲酸脱氢酶D＆E，编码甲酸脱氢酶D＆E的fdhD和fdhE核苷酸序列，重组载体 含有核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

    Gene encoding formate dehydrogenases D and E and method for preparing succinic acid using the same
    3.
    发明授权
    Gene encoding formate dehydrogenases D and E and method for preparing succinic acid using the same 有权
    编码甲酸脱氢酶D和E的基因及使用其制备琥珀酸的方法

    公开(公告)号：US07572615B2

    公开(公告)日：2009-08-11

    申请号：US11738463

    申请日：2007-04-20

    申请人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

    发明人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

    IPC分类号： C12N9/00 ,  C12P21/00

    CPC分类号： C12P7/46 ,  C12N9/0008

    摘要： Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.

    摘要翻译： 编码甲酸脱氢酶D＆E的核苷酸序列和使用其编码琥珀酸的方法，更具体地说，将甲酸转化为二氧化碳和氢的甲酸脱氢酶D＆E，编码甲酸脱氢酶D＆E的fdhD和fdhE核苷酸序列，重组载体 含有核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

    Recombinant mutant microorganisms having increased ability to produce alcohols and method of producing alcohols using the same
    4.
    发明授权
    Recombinant mutant microorganisms having increased ability to produce alcohols and method of producing alcohols using the same 有权
    具有增加生产醇的能力的重组突变体微生物和使用其制备醇的方法

    公开(公告)号：US08765446B2

    公开(公告)日：2014-07-01

    申请号：US13497736

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N9/02 ,  C12N15/00 ,  C12P7/16 ,  C12Q1/26 ,  C07H21/04

    CPC分类号： C12N9/13 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/74 ,  C12P3/00 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  Y02E50/10 ,  Y02E50/17

    摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.

    摘要翻译： 本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以 作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。 生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物 。 由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。 因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

    Gene encoding malic enzyme and method for preparing succinic acid using the same
    7.
    发明授权
    Gene encoding malic enzyme and method for preparing succinic acid using the same 有权
    基因编码苹果酸酶及使用其制备琥珀酸的方法

    公开(公告)号：US07470770B2

    公开(公告)日：2008-12-30

    申请号：US11229368

    申请日：2005-09-16

    申请人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Jeong Wook Lee

    发明人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Jeong Wook Lee

    IPC分类号： C07K14/00 ,  C12N15/11 ,  C12N15/63 ,  C12N1/20 ,  C12P13/20

    CPC分类号： C12N9/0006 ,  C12P7/46

    摘要： A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.

    摘要翻译： 编码苹果酸的核苷酸序列和使用该琥珀酸的琥珀酸的制备方法，特别是编码具有将丙酮酸或丙酮酸转化成苹果酸或苹果酸的活性的苹果酸B的maeB核苷酸序列，反之亦然， 含有该基因的重组载体，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

    RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME
    9.
    发明申请
    RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME 有权
    具有增加生产酒精的能力的重组突变体微生物和使用该醇的生产醇的方法

    公开(公告)号：US20120301936A1

    公开(公告)日：2012-11-29

    申请号：US13497736

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    IPC分类号： C12P7/16 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C12N9/13 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/74 ,  C12P3/00 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  Y02E50/10 ,  Y02E50/17

    摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.

    摘要翻译： 本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以 作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。 生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物 。 由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。 因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。