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    Methods and systems for increasing production of equilibrium reactions
    9.
    发明授权
    Methods and systems for increasing production of equilibrium reactions 失效
    增加平衡反应产生的方法和系统

    公开(公告)号：US07888081B2

    公开(公告)日：2011-02-15

    申请号：US11752492

    申请日：2007-05-23

    申请人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

    发明人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

    IPC分类号： C12P13/22 ,  C12N1/21

    CPC分类号： C07D209/20 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  C12P17/10

    摘要： Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

    摘要翻译： 描述了用于增加平衡反应生成的方法和系统。 在一些实施方案中，一种方法包括一旦达到平衡即从反应混合物中除去酶，选择性地重新加入用于将产物形成反应驱动到产物中的酶和反应物，以及任选地再循环稳定的中间体，副产物和/或副产物 回到反应混合物中。 在一些实施方案中，方法包括从反应混合物中纯化所需产物，并根据需要选择性地将反应混合物中的一种或多种组分（例如原始反应物，中间体，副产物或副产物）循环回到反应混合物中，以改善 滴度。 还提供了实现方法的系统。 在一些实施方案中，实施方法和系统用于增加在多步平衡途径中产生的莫纳甜和莫纳甜衍生物的产生。

    Reduction of the toxic effect of impurities from raw materials by extractive fermentation
    10.
    发明授权
    Reduction of the toxic effect of impurities from raw materials by extractive fermentation 有权
    通过提取发酵降低原料中杂质的毒性作用

    公开(公告)号：US08313934B2

    公开(公告)日：2012-11-20

    申请号：US12239562

    申请日：2008-09-26

    申请人： Monica Bhatia ,  Michael C. M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

    发明人： Monica Bhatia ,  Michael C. M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

    IPC分类号： C12P7/62

    CPC分类号： C12P7/649 ,  Y02E50/13

    摘要： There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

    摘要翻译： 提供了改善来自工程微生物细胞的生物产物生产的生物产物和方法，所述方法包括：提供包含粗碳源的发酵液; 用所述微生物细胞接种所述发酵液; 并培养接种的发酵液; 其中所述生物产物是与所述发酵液不混溶的疏水性溶剂，并且其中存在于所述粗碳源中的有毒副产物可溶于所述疏水溶剂。 此外，提供了用于实践改善生物产物生产方法的试剂盒。