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    Electrode-less dielectrophorises for polarizable particles
    6.
    发明授权
    Electrode-less dielectrophorises for polarizable particles 失效
    用于可极化颗粒的无电极介电性能

    公开(公告)号：US06824664B1

    公开(公告)日：2004-11-30

    申请号：US09707892

    申请日：2000-11-06

    申请人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Edward C. Cox ,  Chia Fu Chou ,  Olgica Bakajin

    发明人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Edward C. Cox ,  Chia Fu Chou ,  Olgica Bakajin

    IPC分类号： C02F140

    CPC分类号： B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2400/0421 ,  B01L2400/0424 ,  B03C5/024 ,  B03C5/026

    摘要： The present invention further provides a device for the integrated micromanipulation, amplification, and analysis of polarized particles such as DNA comprises a microchip which contains constrictions of insulating material for dielectrophoresis powered by an alternating current or direct current signal generator, and attached to a hot source that can be heated to specific temperatures. Nucleic acids can be heated and cooled to allow for denaturation, and the annealing of complementary primers and enzymatic reactions, as in a thermocycling reaction. After such a reaction has been completed at the constriction, the dielectrophoretic field can be switched to a direct field to release the product and direct it through a matrix for fractionation. The device includes data analysis equipment for the control of these operations, and imaging equipment for the analysis of the products. The invention permits the efficient handling of minute samples in large numbers, since reactions occur while sample material is trapped between constrictions. Because the positioning, reactions, and release into a fractioning matrix all occur at the constriction which serves as a focusing locus, the need to transfer samples into different tubes is eliminated, thus increasing the efficiency and decreasing the possibility of damage to the samples.

    摘要翻译： 本发明还提供了用于对诸如DNA的极化粒子进行集成显微操作，放大和分析的装置，包括微芯片，其包含用于由交流电或直流信号发生器供电的介电电泳的绝缘材料的收缩部分，并连接到热源 可以加热到特定温度。 核酸可被加热和冷却以允许变性，以及互补引物的退火和酶反应，如在热循环反应中。 在收缩处完成这样的反应之后，介电电泳场可以切换到直接场以释放产物并将其引导通过基质进行分馏。 该设备包括用于控制这些操作的数据分析设备和用于分析产品的成像设备。 本发明允许有效地处理微量样品，因为在样品材料被捕获在收缩之间时发生反应。 因为定位，反应和释放到分级矩阵中都会发生在用作聚焦轨迹的收缩处，消除了将样品转移到不同管中的需要，从而提高了效率并降低了样品损伤的可能性。

    Optically characterizing polymers
    7.
    发明授权
    Optically characterizing polymers 有权
    光学表征聚合物

    公开(公告)号：US06790671B1

    公开(公告)日：2004-09-14

    申请号：US09373822

    申请日：1999-08-13

    申请人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Eugene Y. Chan

    发明人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Eugene Y. Chan

    IPC分类号： C12Q168

    CPC分类号： B82Y20/00 ,  G01N21/6408 ,  G01N2021/6441 ,  Y10S435/808 ,  Y10T436/143333 ,  Y10T436/25375

    摘要： The invention relates to new systems, methods and products for analyzing polymers and in particular new systems, methods and products useful for obtaining sequence information from polymers. The invention has numerous advantages over prior art systems and methods used to obtain sequence-related information. Using the methods of the invention the entire human genome could be analyzed several orders of magnitude faster than could be accomplished using conventional technology. In addition to obtaining sequencing information for the entire genome, the systems, methods and products of the invention can be used to create comprehensive and multiple expression maps for developmental and disease processes. The ability to analyze an individual's genome and to generate multiple expression maps will greatly enhance the ability to determine the genetic basis of any phenotypic trait or disease process.

    摘要翻译： 本发明涉及用于分析聚合物的新系统，方法和产品，特别是可用于从聚合物获得序列信息的新系统，方法和产品。 本发明与用于获得序列相关信息的现有技术系统和方法相比具有许多优点。 使用本发明的方法，可以将整个人类基因组分析比使用常规技术可以实现的几个数量级。 除了获得整个基因组的测序信息之外，本发明的系统，方法和产品可用于为发育和疾病过程创建综合和多重表达图。 分析个体基因组和产生多个表达图谱的能力将大大提高确定任何表型性状或疾病过程的遗传基础的能力。