-
公开(公告)号：US5658753A
公开(公告)日：1997-08-19
申请号：US402158
申请日：1995-03-09
CPC分类号： C12N9/0002
摘要： Catalytic antibody components, methods for producing catalytic antibody components, methods for using catalytic antibody components, in particular, single chain and smaller components are disclosed. Catalytic antibody components able to promote the cleavage or formation of an amide, peptide, ester or glycosidic bond, and which are prepared from monoclonal catalytic antibodies, catalytic autoantibodies or by site-directed mutagenesis are disclosed. Methods of using catalytic antibody components alone or in combination with other antibody components or other biological moieties are disclosed.
摘要翻译： 公开了催化抗体组分，用于制备催化抗体组分的方法，使用催化抗体组分的方法，特别是单链和较小组分。 公开了能够促进切割或形成酰胺，肽，酯或糖苷键的催化抗体组分，其由单克隆催化抗体，催化自身抗体或通过定点诱变制备。 公开了单独使用催化抗体组分或与其它抗体组分或其它生物学部分组合的方法。
-
公开(公告)号：US5229272A
公开(公告)日：1993-07-20
申请号：US498225
申请日：1990-03-23
IPC分类号： A61K39/395 , C07K1/113 , C07K1/22 , C07K14/00 , C07K14/52 , C07K14/54 , C07K14/55 , C07K14/575 , C07K14/705 , C07K16/00 , C07K16/06 , C07K16/26 , C07K16/44 , C07K19/00 , C12N9/00 , C12N9/48 , C12N15/09 , C12P21/02 , C12P21/08 , C12R1/19 , C12R1/91
CPC分类号： C07K14/57563 , C07K14/55 , C07K16/06 , C07K16/26 , C07K16/44 , C12N9/0002 , C12N9/48 , C07K2319/00 , C07K2319/75
摘要： Catalytic antibody components, methods for producing catalytic antibody components, methods for using catalytic antibody components, in particular, single chain and smaller components are disclosed. Catalytic antibody components able to promote the cleavage or formation of an amide, peptide, ester or glycosidic bond, and which are prepared from monoclonal catalytic antibodies, catalytic autoantibodies or by site-directed mutagenesis are disclosed. Methods of using catalytic antibody components alone or in combination with other antibody components or other biological moieties are disclosed.
摘要翻译： 公开了催化抗体组分，用于制备催化抗体组分的方法，使用催化抗体组分的方法，特别是单链和较小组分。 公开了能够促进切割或形成酰胺，肽，酯或糖苷键的催化抗体组分，其由单克隆催化抗体，催化自身抗体或通过定点诱变制备。 公开了单独使用催化抗体组分或与其它抗体组分或其它生物学部分组合的方法。
-
3.发明授权公开(公告)号：US06881589B1
公开(公告)日：2005-04-19
申请号：US08465443
申请日：1995-06-05
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/543 , G01N33/58 , G01N33/53 , G01N21/00 , G01N33/544 , G01N33/566
CPC分类号： C12Q1/6825 , C07H21/00 , C12Q1/6816 , C12Q1/686 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N2458/30 , Y10S436/805 , Y10S436/806 , C12Q2565/628 , C12Q2563/113 , C12Q2563/103
摘要： The invention provides compositions and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence. The compositions and kits comprise an electrochemiluminescent label, collectable particles, binding reagents, and an electrolyte.
摘要翻译： 本发明提供了基于电化学发光对样品中存在的感兴趣分析物进行结合测定的组合物和试剂盒。 组合物和试剂盒包括电化学发光标记，可收集颗粒，结合试剂和电解质。
-
4.发明授权Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets 失效包括多个磁体的基于磁性微粒的发光测定方法和装置
公开(公告)号：US5705402A
公开(公告)日：1998-01-06
申请号：US255824
申请日：1994-06-08
申请人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： Disclosed and claimed are methods and apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode. The method uses magnetically responsive particles. The method and apparatus call for a plurality of electromagnets or permanent magnets in north-south orientation for imposing a magnetic field so as to collect the particles.
摘要翻译： 公开并要求保护的是基于电极上的电化学发光测量，对样品中存在的感兴趣分析物进行结合测定的方法和装置。 该方法使用磁响应颗粒。 所述方法和装置要求用于施加磁场以收集颗粒的南北取向的多个电磁体或永磁体。
-
公开(公告)号：US06325973B1
公开(公告)日：2001-12-04
申请号：US09253558
申请日：1999-02-19
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： G01N2726
摘要： An apparatus for performing a binding assay for an analyte of interest in a sample based upon measurement of clectrochemiluminescence at an electrode surface comprising: (a) a cell defining a sample; (b) an electrode adjacent a portion of the sample containing volume; (c) a voltage control device for impressing electrochemical energy upon the electrode sufficient to generate luminescence; (d) means for magnetically collecting particles along the electrode surface; and (e) a light detection device for measuring the luminescence.
摘要翻译： 一种用于基于电极表面上的电化学发光测量来对样品中感兴趣的分析物进行结合测定的装置，包括：（a）限定样品的细胞; （b）邻近样品容纳体积的一部分的电极; （c）电压控制装置，用于在足以产生发光的电极上施加电化学能; （d）沿着电极表面磁性收集颗粒的装置; 和（e）用于测量发光的光检测装置。
-
公开(公告)号：US5935779A
公开(公告)日：1999-08-10
申请号：US346832
申请日：1994-11-30
申请人： Richard J. Massey , Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn
发明人： Richard J. Massey , Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/58
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/686 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemilurninescence is related to the amount of analyte in the sample.
摘要翻译： 本发明涉及基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法。 在该方法中使用颗粒，然后将其收集在可以诱导电化学发光的区域中，其中诱导的电化学发光的量与样品中分析物的量相关。
-
公开(公告)号：US6078782A
公开(公告)日：2000-06-20
申请号：US473313
申请日：1995-06-07
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/58 , G01N33/553
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/686 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemiluminescence is related to the amount of analyte in the sample.
摘要翻译： 本发明涉及基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法。 在该方法中使用颗粒，然后将其收集在可以诱导电化学发光的区域中，其中诱导的电化学发光的量与样品中分析物的量相关。
-
公开(公告)号：US07462322B2
公开(公告)日：2008-12-09
申请号：US10912574
申请日：2004-08-06
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： G01N21/76 , G01N33/553 , G01N27/26 , C12M1/00
CPC分类号： G01N33/582 , C07H21/00 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438
摘要： An apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising a cell defining a sample containing volume intersecting with inlet and outlet means, an electrode having a substantially horizontally positioned surface exposed to and positioned below a portion of the sample containing volume, means for impressing electrochemical energy upon said electrode sufficient to generate luminescence, means for magnetically collecting particles along said surface and means for measuring the luminescence generated at said electrode.
摘要翻译： 基于在包括限定与入口和出口装置相交的体积的样品的细胞的电极表面上测量电化学发光，对样品中存在的感兴趣分析物进行结合测定的装置，具有暴露于 并且位于所述样品容纳体积的一部分下方，用于在所述电极上施加足以产生发光的电化学能量的装置，用于沿着所述表面磁性收集颗粒的装置和用于测量在所述电极处产生的发光的装置。
-
公开(公告)号：US5962218A
公开(公告)日：1999-10-05
申请号：US160063
申请日：1993-11-24
申请人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Gary F. Blackburn , Richard J. Massey
发明人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Gary F. Blackburn , Richard J. Massey
IPC分类号： C12Q1/68 , G01N1/40 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/553
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/5438 , G01N1/40
摘要： The invention relates to methods, apparatus, reagents, and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. In the method, reagents and kits particles can be employed; for instance, for settling upon the electrode surface by gravity, centrifugation or magnetic attraction. The apparatus can include a magnet for generating a magnetic field in a region proximate the electrode.
摘要翻译： 本发明涉及用于基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法，装置，试剂和试剂盒。 在该方法中，可以使用试剂和试剂盒颗粒; 例如，通过重力，离心或磁吸引来沉积在电极表面上。 该装置可以包括用于在靠近电极的区域中产生磁场的磁体。
-
公开(公告)号：US6087476A
公开(公告)日：2000-07-11
申请号：US906654
申请日：1997-08-07
申请人： John H. Kenten , Jan Casadei , Michael J. Powell
发明人： John H. Kenten , Jan Casadei , Michael J. Powell
IPC分类号： C07K14/16 , C07K14/435 , C07K16/18 , C07K16/44 , C12N15/62 , G01N33/533 , G01N33/58 , C07K14/00 , C12N9/00
CPC分类号： C07K14/005 , C07K14/43595 , C07K16/18 , C07K16/44 , C12N15/62 , G01N33/533 , G01N33/582 , C07K2319/00 , C07K2319/40 , C07K2319/60 , C07K2319/735 , C12N2740/16122
摘要： The invention relates to luminescent chimeric proteins which include a photoprotein and a second protein which may be light- or heavy-chain immunoglobulin, an antigenic peptide, avidin, streptavidin, or protein A. The invention also relates to chimeric protein genes, plasmids containing said gene, and hosts transformed with said plasmid. The invention also relates to a range of highly sensitive immunoassays which use the chimeric proteins.
摘要翻译： 本发明涉及发光嵌合蛋白，其包括光蛋白和可以是轻链或重链免疫球蛋白，抗原肽，抗生物素蛋白，链霉亲和素或蛋白A的第二蛋白。本发明还涉及嵌合蛋白基因，含有所述 基因和用所述质粒转化的宿主。 本发明还涉及使用嵌合蛋白的一系列高度灵敏的免疫测定。
-
-
-
-
-
-
-
-
-
搜索结果
43 条记录 (耗时 0.036 秒)
