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公开(公告)号:US5658753A
公开(公告)日:1997-08-19
申请号:US402158
申请日:1995-03-09
CPC分类号: C12N9/0002
摘要: Catalytic antibody components, methods for producing catalytic antibody components, methods for using catalytic antibody components, in particular, single chain and smaller components are disclosed. Catalytic antibody components able to promote the cleavage or formation of an amide, peptide, ester or glycosidic bond, and which are prepared from monoclonal catalytic antibodies, catalytic autoantibodies or by site-directed mutagenesis are disclosed. Methods of using catalytic antibody components alone or in combination with other antibody components or other biological moieties are disclosed.
摘要翻译: 公开了催化抗体组分,用于制备催化抗体组分的方法,使用催化抗体组分的方法,特别是单链和较小组分。 公开了能够促进切割或形成酰胺,肽,酯或糖苷键的催化抗体组分,其由单克隆催化抗体,催化自身抗体或通过定点诱变制备。 公开了单独使用催化抗体组分或与其它抗体组分或其它生物学部分组合的方法。
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公开(公告)号:US5229272A
公开(公告)日:1993-07-20
申请号:US498225
申请日:1990-03-23
IPC分类号: A61K39/395 , C07K1/113 , C07K1/22 , C07K14/00 , C07K14/52 , C07K14/54 , C07K14/55 , C07K14/575 , C07K14/705 , C07K16/00 , C07K16/06 , C07K16/26 , C07K16/44 , C07K19/00 , C12N9/00 , C12N9/48 , C12N15/09 , C12P21/02 , C12P21/08 , C12R1/19 , C12R1/91
CPC分类号: C07K14/57563 , C07K14/55 , C07K16/06 , C07K16/26 , C07K16/44 , C12N9/0002 , C12N9/48 , C07K2319/00 , C07K2319/75
摘要: Catalytic antibody components, methods for producing catalytic antibody components, methods for using catalytic antibody components, in particular, single chain and smaller components are disclosed. Catalytic antibody components able to promote the cleavage or formation of an amide, peptide, ester or glycosidic bond, and which are prepared from monoclonal catalytic antibodies, catalytic autoantibodies or by site-directed mutagenesis are disclosed. Methods of using catalytic antibody components alone or in combination with other antibody components or other biological moieties are disclosed.
摘要翻译: 公开了催化抗体组分,用于制备催化抗体组分的方法,使用催化抗体组分的方法,特别是单链和较小组分。 公开了能够促进切割或形成酰胺,肽,酯或糖苷键的催化抗体组分,其由单克隆催化抗体,催化自身抗体或通过定点诱变制备。 公开了单独使用催化抗体组分或与其它抗体组分或其它生物学部分组合的方法。
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公开(公告)号:US5194585A
公开(公告)日:1993-03-16
申请号:US486594
申请日:1990-02-28
IPC分类号: A61K35/16 , A61K38/00 , A61K39/395 , A61P3/08 , A61P11/00 , A61P11/06 , A61P13/02 , A61P15/00 , A61P37/00 , A61P37/06 , C07K1/22 , C07K7/06 , C07K14/435 , C07K14/575 , C07K16/00 , C07K16/06 , C07K16/18 , C07K16/26 , C12N9/00 , C12N9/48 , C12N9/99 , C12P21/08 , G01N33/53 , G01N33/564
CPC分类号: C07K16/06 , C07K14/57563 , C07K16/18 , C07K16/26 , C12N9/0002 , C12N9/48 , A61K38/00 , C07K2317/34 , Y10S424/81 , Y10S530/868
摘要: Specific, selective inhibitors of catalytic antibodies both synthetic and naturally occurring, their use and compositions thereof are disclosed. In particular, an inhibitor preventing the hydrolysis of the peptide bond between amino acid residues 16 and 17 in the neurotransmitter vasoactive intestinal peptide (VIP) by an anti-VIP catalytic autoantibody is disclosed.
摘要翻译: 公开了特定的合成和天然存在的催化抗体的选择性抑制剂,其用途和组合物。 具体地,公开了通过抗VIP催化剂自身抗体阻止神经递质血管活性肠肽(VIP)中的氨基酸残基16和17之间的肽键水解的抑制剂。
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公开(公告)号:US6048717A
公开(公告)日:2000-04-11
申请号:US478285
申请日:1995-06-07
CPC分类号: C07K16/06 , C07K14/57563 , C07K16/18 , C07K16/26 , C12N9/0002 , C12N9/48 , A61K38/00 , C07K2317/34
摘要: Specific, selective inhibitors of catalytic antibodies both synthetic and naturally occurring, their use and compositions thereof are disclosed. In particular, an inhibitor preventing the hydrolysis of the peptide bond between amino acid residues 16 and 17 in the neurotransmitter vasoactive intestinal peptide (VIP) by an anti-VIP catalytic autoantibody is disclosed.
摘要翻译: 公开了特定的合成和天然存在的催化抗体的选择性抑制剂,其用途和组合物。 具体地,公开了通过抗VIP催化剂自身抗体阻止神经递质血管活性肠肽(VIP)中的氨基酸残基16和17之间的肽键水解的抑制剂。
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公开(公告)号:US6087476A
公开(公告)日:2000-07-11
申请号:US906654
申请日:1997-08-07
申请人: John H. Kenten , Jan Casadei , Michael J. Powell
发明人: John H. Kenten , Jan Casadei , Michael J. Powell
IPC分类号: C07K14/16 , C07K14/435 , C07K16/18 , C07K16/44 , C12N15/62 , G01N33/533 , G01N33/58 , C07K14/00 , C12N9/00
CPC分类号: C07K14/005 , C07K14/43595 , C07K16/18 , C07K16/44 , C12N15/62 , G01N33/533 , G01N33/582 , C07K2319/00 , C07K2319/40 , C07K2319/60 , C07K2319/735 , C12N2740/16122
摘要: The invention relates to luminescent chimeric proteins which include a photoprotein and a second protein which may be light- or heavy-chain immunoglobulin, an antigenic peptide, avidin, streptavidin, or protein A. The invention also relates to chimeric protein genes, plasmids containing said gene, and hosts transformed with said plasmid. The invention also relates to a range of highly sensitive immunoassays which use the chimeric proteins.
摘要翻译: 本发明涉及发光嵌合蛋白,其包括光蛋白和可以是轻链或重链免疫球蛋白,抗原肽,抗生物素蛋白,链霉亲和素或蛋白A的第二蛋白。本发明还涉及嵌合蛋白基因,含有所述 基因和用所述质粒转化的宿主。 本发明还涉及使用嵌合蛋白的一系列高度灵敏的免疫测定。
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公开(公告)号:US06846654B1
公开(公告)日:2005-01-25
申请号:US08090245
申请日:1993-07-09
IPC分类号: C07K16/10 , C07K16/42 , C07K16/44 , C12N9/00 , C12P1/00 , C12P13/22 , C12P17/10 , C12P19/34 , C12P41/00 , C12Q1/25 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/544 , G01N33/53 , C07K16/00
CPC分类号: B82Y5/00 , C07K16/1063 , C07K16/4241 , C07K16/44 , C12N9/0002 , C12P1/00 , C12P13/227 , C12P17/10 , C12P19/34 , C12P41/004 , C12Q1/25 , G01N33/535 , G01N33/542 , G01N33/5438 , G01N33/544 , G01N2333/16 , G01N2333/96472 , Y10S435/817
摘要: A system for detecting physical or chemical changes in an environment is disclosed. The system comprises means for conducting a chemical reaction; an active biological molecule whose physiological function is noncatalytic which is capable of catalyzing a chemical reaction wherein at least one reactant is converted to one product; and means for sensing and processing information relating to changes in said environment. A method for detecting physical or chemical changes in an environment is also disclosed.
摘要翻译: 公开了一种用于检测环境中物理或化学变化的系统。 该系统包括用于进行化学反应的装置; 活性生物分子,其生理功能是非催化的,其能够催化化学反应,其中至少一种反应物转化为一种产物; 以及用于感测和处理与所述环境的变化有关的信息的装置。 还公开了一种用于检测环境中的物理或化学变化的方法。
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公开(公告)号:US5716781A
公开(公告)日:1998-02-10
申请号:US470247
申请日:1995-06-06
申请人: Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
发明人: Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
IPC分类号: C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C12Q1/00 , C12Q1/70 , G01N33/48
CPC分类号: C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要: Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译: 具有式[Re(P)m(L1)n(L2)o(L3)p(L4)q(L5)r(L6)s] t(B)u的电化学发光部分其中P是Re的多齿配体; L1,L2,L3,L4,L5和L6是Re的配体,其各自可以彼此相同或不同; B是作为Re的配体或与P,L1,L2,L3,L4,L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n,o,p,q,r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P,L1,L2,L3,L4,L5,L6和B具有这样的组成和数量,可以诱导化学部分发射电磁辐射,并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合,将所得样品暴露于化学,电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。
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公开(公告)号:US5952462A
公开(公告)日:1999-09-14
申请号:US333237
申请日:1994-11-02
CPC分类号: B82Y5/00 , C07C237/22 , C07K16/1063 , C12N9/0002 , C12Q1/25 , G01N2333/16 , G01N2333/96472
摘要: Antigens capable of eliciting antibodies which can catalyze chemical reactions, in particular, the cleavage or formation of a peptide linkage, comprising a hapten or a hapten and a suitable carrier molecule are disclosed. Haptens include, among others, silicon and boron containing compounds. Antibodies which are catalytically active for chemical reactions, in particular, the cleavage or formation of a selected peptide linkage or an ester bond, and which are elicited by such antigens are disclosed as well as methods for producing the antibodies and methods for catalyzing the cleavage or formation of a peptide linkage or in ester bond in a molecule.
摘要翻译: 公开了能够引发抗体的抗原,其可以催化化学反应,特别是包含半抗原或半抗原和合适的载体分子的肽键的切割或形成。 除了其他之外,半抗原包括含有硅和硼的化合物。 公开了对于化学反应具有催化活性的抗体,特别是选择的肽键或酯键的切割或形成,并且由这些抗原引发的抗体以及用于产生抗体的方法和用于催化切割的方法 在分子中形成肽键或酯键。
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公开(公告)号:US5811236A
公开(公告)日:1998-09-22
申请号:US468524
申请日:1995-06-06
申请人: Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
发明人: Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
IPC分类号: C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C07H21/04 , C07K15/28 , G01N23/53
CPC分类号: C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要: Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译: 具有式[Re(P)m(L1)n(L2)o(L3)p(L4)q(L5)r(L6)s] t(B)u的电化学发光部分,其中P是Re的多齿配体; L1,L2,L3,L4,L5和L6是Re的配体,其各自可以彼此相同或不同; B是作为Re的配体或与P,L1,L2,L3,L4,L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n,o,p,q,r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P,L1,L2,L3,L4,L5,L6和B具有这样的组成和数量,可以诱导化学部分发射电磁辐射,并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合,将所得样品暴露于化学,电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。
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公开(公告)号:US06521432B1
公开(公告)日:2003-02-18
申请号:US08479849
申请日:1995-06-07
申请人: Michael J. Powell , Anthony R. Rees , Paul M. Booth , Wonpyo Hong , Richard C. Titmas , Richard J. Massey
发明人: Michael J. Powell , Anthony R. Rees , Paul M. Booth , Wonpyo Hong , Richard C. Titmas , Richard J. Massey
IPC分类号: C12N900
CPC分类号: C07D207/404 , C07C229/46 , C07C229/48 , C07C235/74 , C07C271/24 , C07C311/06 , C07C317/44 , C07C2601/04 , C07C2602/42 , C07D205/085 , C07F9/2458 , C07F9/301 , C07F9/3211 , C07F9/4006 , C07F9/5537 , C07K7/02 , C07K14/005 , C07K16/00 , C07K16/1063 , C07K16/241 , C07K16/4291 , C12N9/0002 , C12N2740/16122 , C12Q1/25 , G01N33/531 , G01N33/577 , G01N2333/16 , G01N2333/96472
摘要: Antigens capable of eliciting antibodies which can enhance the rate of chemical reactions at peptide bonds are disclosed. In particular, the rate of cleavage or formation of metastable peptide bonds, such as ASN-X, ASP-X, GLN-X, GLU-X, LYS-X, and HIS-Y-X, where X and Y are any amino acid, is enhanced by antibodies elicited by said antigen.
摘要翻译: 公开了能够引发可增加肽键化学反应速率的抗体的抗原。 特别地,其中X和Y是任何氨基酸的亚稳态肽键的切割或形成速率,例如ASN-X,ASP-X,GLN-X,GLU-X,LYS-X和HIS-YX, 由所述抗原引起的抗体增强。
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