公开(公告)号：US09395109B2

公开(公告)日：2016-07-19

申请号：US13699804

申请日：2010-05-26

申请人： Terry A. Berger ,  Kimber Fogelman

发明人： Terry A. Berger ,  Kimber Fogelman

IPC分类号： F25B21/02 ,  F04B39/06 ,  F04B53/08 ,  B01D15/40 ,  G01N30/32

CPC分类号： F25B21/04 ,  B01D15/12 ,  B01D15/40 ,  F04B39/064 ,  F04B53/08 ,  F25B21/02 ,  G01N2030/326

摘要： A system for chilling a pumping system includes a Peltier cooling element, and first and second heat exchangers. The Peltier element is arranged near a pump head of a pump such that the Peltier element and the pump head are in thermal contact, where the Peltier element chills the pump head and a mobile phase flowstream prior to fluid from the mobile phase flowstream entering the pump. The first heat exchanger is attached to the Peltier element, and removes heat from the Peltier element using a circulating fluid. The second heat exchanger cools the circulating fluid, where the Peltier element and the pump head are arranged in an area that is isolated from the second heat exchanger.

摘要翻译： 用于冷却泵送系统的系统包括珀尔帖冷却元件和第一和第二热交换器。 珀尔帖元件设置在泵的泵头附近，使得珀尔帖元件和泵头处于热接触状态，其中珀尔帖元件在流入流入泵的流动相流动之前，使泵头和流动相流动流动 。 第一个热交换器连接到珀尔帖元件，并使用循环流体从珀尔帖元件中除去热量。 第二热交换器冷却循环流体，其中珀耳帖元件和泵头被布置在与第二热交换器隔离的区域中。

公开(公告)号：US20140190183A1

公开(公告)日：2014-07-10

申请号：US13699804

申请日：2010-05-26

申请人： Terry A. Berger ,  Kimber Fogelman

发明人： Terry A. Berger ,  Kimber Fogelman

IPC分类号： F25B21/02

CPC分类号： F25B21/04 ,  B01D15/12 ,  B01D15/40 ,  F04B39/064 ,  F04B53/08 ,  F25B21/02 ,  G01N2030/326

摘要： Methods and systems for pumping compressible fluids in high pressure applications such as high-pressure liquid chromatography (HPLC) or supercritical fluid chromatography (SFC) applications are disclosed. An improved cooling device for a pump head for use in a supercritical fluid chromatography (SFC) system is described. A system for chilling a pumping system, includes a Peltier cooling element in thermal contact with a pump head, wherein the cooling element chills the pump head and a mobile phase fluid flowstream prior to the mobile phase fluid entering the pump; a fluid-cooled heat exchanger, attached to the Peltier cooling element, which removes heat from the cooling element using a circulating fluid; and a second heat exchanger which cools the circulating fluid.

摘要翻译： 公开了用于在高压液相色谱（HPLC）或超临界流体色谱（SFC）应用中的高压应用中泵送可压缩流体的方法和系统。 描述了用于超临界流体色谱（SFC）系统的用于泵头的改进的冷却装置。 用于冷却泵送系统的系统包括与泵头热接触的珀尔帖冷却元件，其中在移动相流体进入泵之前，冷却元件使泵头和流动相流体流动冷却; 附接到珀尔帖冷却元件的流体冷却的热交换器，其使用循环流体从冷却元件移除热量; 以及冷却循环流体的第二热交换器。

公开(公告)号：US06652753B2

公开(公告)日：2003-11-25

申请号：US10067011

申请日：2002-02-04

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

IPC分类号： C02F144

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

公开(公告)号：US4982597A

公开(公告)日：1991-01-08

申请号：US377534

申请日：1989-07-10

申请人： Terry A. Berger

发明人： Terry A. Berger

IPC分类号： G01N30/02 ,  G01N30/30 ,  G01N30/60 ,  G01N30/68

CPC分类号： G01N30/68 ,  G01N30/30 ,  G01N2030/3015 ,  G01N2030/3038 ,  G01N2030/6008 ,  G01N2030/6013 ,  G01N30/02

摘要： In a system for analyzing a chemical sample by chromatographic separation of said sample into components a method and apparatus are provided for heating the mobile phase to a desired temperature prior to exiting the restrictor, while maintaining the density of the mobile phase at a desired level. Such heating and density maintenance being provided by the combination of a restrictor having a minimal taper length and a temperature zone immediately prior to the outlet end of the restrictor, which temperature zone has a minimum length. A second temperature zone spaced upstream from the first zone, and an intermediate thermally conductive member between the first and second zones can be provided as an alternative embodiment for establishing a thermal gradient. As the mobile phase passes through the second zone, the intermediate conductive member, and the first zone, the mobile phase is subjected to a thermal gradient.

摘要翻译： 在用于通过将所述样品色谱分离成组分来分析化学样品的系统中，提供了一种方法和装置，用于在将流动相的密度保持在期望水平之前将流动相加热至所需温度，然后再离开限流器。 这种加热和密度维持是通过具有最小锥度长度的限流器和紧邻限流器出口端之前的温度区域的组合提供的，该温度区域具有最小长度。 作为建立热梯度的替代实施例，可以提供在第一区域上游隔开的第二温度区域和位于第一区域和第二区域之间的中间导热构件。 当流动相通过第二区时，中间导电构件和第一区，流动相受到热梯度的影响。

公开(公告)号：US4845985A

公开(公告)日：1989-07-11

申请号：US157344

申请日：1988-02-17

申请人： Terry A. Berger

发明人： Terry A. Berger

IPC分类号： G01N30/02 ,  B01D11/00 ,  G01N30/28 ,  G01N30/30 ,  G01N30/32

CPC分类号： G01N30/30 ,  G01N30/28 ,  G01N2030/3038 ,  G01N2030/3076 ,  G01N2030/324 ,  G01N30/02

摘要： In a system for analyzing a chemical sample by chromatographic separation of said sample into components a method and apparatus are provided for maximizing detector resolution and minimizing duration by maintaining the linear velocity of the mobile phase at a desired level by affecting the viscosity and density characteristics of the mobile phase as it passes through a restrictor by programming the restrictor temperature.

公开(公告)号：US09163618B2

公开(公告)日：2015-10-20

申请号：US13530931

申请日：2012-06-22

申请人： Edwin E. Wikfors ,  Kimber D. Fogelman ,  Terry A. Berger

发明人： Edwin E. Wikfors ,  Kimber D. Fogelman ,  Terry A. Berger

IPC分类号： G01N1/00 ,  F04B11/00 ,  F04B23/06 ,  G01N30/32 ,  G01N30/34

CPC分类号： F04B11/0075 ,  F04B23/06 ,  G01N30/32 ,  G01N30/34 ,  G01N2030/326 ,  Y10T137/0352 ,  Y10T137/7722 ,  G01N2030/342 ,  B01D15/40

摘要： An apparatus, system, and process of converting a standard, high performance liquid chromatography (HPLC) flow path to a flow path suitable for supercritical fluid chromatography (SFC) are described. This reversible technique is applied to a variety of flow configurations including binary, high pressure solvent mixing systems and quaternary, low pressure solvent mixing systems than can be conventionally operated or automated. The technique is generally applied to the fields of supercritical fluid chromatography and high pressure liquid chromatography, but users skilled in the art will find utility for any flow system where pressurization components must be periodically applied to and removed from both ends of a flow stream in an automated manner.

摘要翻译： 描述了将标准的高效液相色谱（HPLC）流动路径转换到适用于超临界流体色谱（SFC）的流动路径的装置，系统和方法。 这种可逆技术应用于各种流动配置，包括二元，高压溶剂混合系统和四级，低压溶剂混合系统，比常规操作或自动化。 该技术通常应用于超临界流体色谱和高压液相色谱领域，但是本领域技术人员将发现对于任何流动系统都将发挥效用，其中加压组分必须周期性地施加到流动流的两端并从其中移除 自动化方式。

公开(公告)号：US06656354B2

公开(公告)日：2003-12-02

申请号：US10066955

申请日：2002-02-04

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth J. Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth J. Klein

IPC分类号： B01D1508

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

公开(公告)号：US06576125B2

公开(公告)日：2003-06-10

申请号：US09931238

申请日：2001-08-17

申请人： Terry A. Berger ,  Kimber D. Fogelman

发明人： Terry A. Berger ,  Kimber D. Fogelman

IPC分类号： B01D1508

CPC分类号： B01D15/166 ,  B01D15/14 ,  B01D15/40 ,  G01N30/02 ,  G01N30/22 ,  G01N30/34 ,  G01N30/36 ,  G01N2030/324 ,  G01N2030/347 ,  G01N30/16

摘要： An improved method of introducing sample into a low-pressure flow stream of a high-pressure chromatography system to improve efficiency and significantly shorten the time of the separation process through a chromatography column is described. The method comprises injecting samples in a modifier liquid flow stream upstream of a mixing column that combines two independent flow streams of a liquid modifier with a highly compressed solvating fluid. Focusing can be controlled at the column head by changing the ratios of the two flow streams. Total flow may also be adjusted to speed the application of the sample onto the column during injection. The method improves the timing and efficiency of a supercritical fluid chromatography system and can reduce the amount of modifier added to the mobile phase flow stream.

摘要翻译： 描述了将样品引入高压色谱系统的低压流动流以提高效率并显着缩短通过色谱柱的分离过程的时间的改进方法。 该方法包括将样品注入混合塔上游的改性剂液流流中，该混合塔将液体改性剂的两个独立流动流与高度压缩的溶剂化流体相结合。 可以通过改变两个流动流的比例来在柱头处控制聚焦。 也可以调整总流量以加速样品在注射期间在柱上的应用。 该方法提高了超临界流体色谱系统的时间和效率，并且可以减少添加到流动相流动流中的改性剂的量。

公开(公告)号：US06558540B2

公开(公告)日：2003-05-06

申请号：US09917210

申请日：2001-07-30

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： B01D1508

CPC分类号： B01D11/0203 ,  B01D15/247 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： An efficient and safe method and apparatus for collecting exhaust gasses in an open-air sample collection container in a supercritical fluid chromatography system is claimed. The invention uses a vacuum force to evacuate the gaseous phase and vapors separated from liquid mobile phase in a sample collection device. The invention is portable to use on a series of collection containers, such as test tubes or wells in a titration place. Embodiments are claimed for capture of gasses and vapors either internally or externally to a collection container. The invention removes the gasses from the collection container that are then safely vented to a waste collector, thereby creating a safer environment for the laboratory and properly removing and disposing potentially hazardous chemicals instead of venting these chemicals into the environment.

摘要翻译： 一种用于在超临界流体色谱系统中的露天样品收集容器中收集废气的有效和安全的方法和装置。 本发明使用真空力来抽空样品收集装置中的气相和与液体流动相分离的蒸汽。 本发明便携式用于一系列收集容器，例如滴定处的试管或孔。 要求用于在收集容器的内部或外部捕集气体和蒸气的实施例。 本发明从收集容器中除去气体，然后将其安全地排放到废物收集器，从而为实验室创造更安全的环境，并适当地去除和处置潜在的危险化学品，而不是将这些化学物质排放到环境中。

公开(公告)号：US06428702B1

公开(公告)日：2002-08-06

申请号：US09918435

申请日：2001-08-01

申请人： Terry A. Berger ,  Kimber D. Fogelman

发明人： Terry A. Berger ,  Kimber D. Fogelman

IPC分类号： B01D1100

CPC分类号： B01D15/14 ,  B01D15/40 ,  G01N30/02 ,  G01N30/16

摘要： An improved method and apparatus for safely introducing sample into a flow stream of a supercritical fluid chromatography system is described. The sample injection method improves safety of injection by eliminating the risk of injecting sample into a high-pressure system containing liquids and gasses at or near supercritical ranges. The method improves the efficiency and significantly shortens the time of the injection process by using a check valve placed between a fill port on an injection valve and sample port where sample is injected by a syringe. After sample injection from a sample loop into a high-pressure flow stream, an injection valve is reset to receive a subsequent sample. High-pressure flow stream mixtures become trapped within the sample loop when the valve is reset. The check valve prevents the mixtures from expanding into the sample port and blowing out a syringe and flow stream mixtures from the fill lines. The trapped flow stream mixtures are directed to a waste port where they are safely contained. The technique can be applied equally as effective to preparatory SFC as well as to analytical SFC systems.

摘要翻译： 描述了用于将样品安全地引入超临界流体色谱系统的流动流的改进的方法和装置。 样品注射方法通过消除在超临界范围内或附近含有液体和气体的高压系统中注入样品的风险，提高了注射的安全性。 该方法通过使用放置在注射阀上的填充口和通过注射器注射样品的样品端口的样品端口之间的止回阀来提高效率并显着缩短注射过程的时间。 从样品回路进样到高压流动流中后，重新注入进样阀以接收随后的样品。 当阀门复位时，高压流动流混合物将被捕获在样品回路内。 止回阀防止混合物膨胀到样品端口，吹出注射器并从填充线流出混合物。 被捕获的流动流混合物被引导到废物端口，在那里它们被安全地包含。 该技术可以同样适用于预备性证监会以及分析SFC系统。