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公开(公告)号：US11702649B2
公开(公告)日：2023-07-18
申请号：US16758640
申请日：2018-10-23
发明人： Paul Blainey , Navpreet Ranu , Todd Gierahn , J. Christopher Love
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869 , C40B30/04 , C40B50/06 , C12N1/20 , C40B40/06 , C40B40/10 , C12R1/465
CPC分类号： C12N15/1006 , C12N1/205 , C12Q1/6806 , C12Q1/6869 , C40B30/04 , C40B40/06 , C40B40/10 , C40B50/06 , C12N2310/20 , C12R2001/465 , C12Y301/11 , C12Y302/02027
摘要： The present invention relates to the analysis of complex single cell sequencing libraries. Disclosed are methods for enrichment of library members based on the presence of cell-of origin barcodes to identify and concentrate DNA that is relevant to interesting cells or components that would be expensive or difficult to study otherwise. Also, disclosed are methods of capturing cDNA library molecules by use of CRISPR systems, hybridization or PCR. The present invention allows for identifying the properties of rare cells in single cell RNA-seq data and accurately profile them through clustering approaches. Further information on transcript abundances from subpopulations of single cells can be analyzed at a lower sequencing effort. The methods also allow for linking TCR alpha and beta chains at the single cell level.
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公开(公告)号：US20240084287A1
公开(公告)日：2024-03-14
申请号：US18327345
申请日：2023-06-01
发明人： Paul Blainey , Navpreet Ranu , Todd Gierahn , J. Christopher Love
IPC分类号： C12N15/10 , C12N1/20 , C12Q1/6806 , C12Q1/6869 , C40B30/04 , C40B40/06 , C40B40/10 , C40B50/06
CPC分类号： C12N15/1006 , C12N1/205 , C12Q1/6806 , C12Q1/6869 , C40B30/04 , C40B40/06 , C40B40/10 , C40B50/06 , C12N2310/20 , C12R2001/465 , C12Y301/11 , C12Y302/02027
摘要： The present invention relates to the analysis of complex single cell sequencing libraries. Disclosed are methods for enrichment of library members based on the presence of cell-of origin barcodes to identify and concentrate DNA that is relevant to interesting cells or components that would be expensive or difficult to study otherwise. Also, disclosed are methods of capturing cDNA library molecules by use of CRISPR systems, hybridization or PCR. The present invention allows for identifying the properties of rare cells in single cell RNA-seq data and accurately profile them through clustering approaches. Further information on transcript abundances from subpopulations of single cells can be analyzed at a lower sequencing effort. The methods also allow for linking TCR alpha and beta chains at the single cell level.
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公开(公告)号：US20190005304A1
公开(公告)日：2019-01-03
申请号：US16102118
申请日：2018-08-13
IPC分类号： G06K9/00 , G01N33/483 , G01N15/14 , G01N35/00
CPC分类号： G06K9/00147 , B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/0896 , B01L2300/12 , G01N15/1425 , G01N15/1463 , G01N33/4833 , G01N35/00029 , G01N35/00871 , G01N2015/0065 , G01N2015/1006 , G01N2035/00148 , G02B21/16 , G02B21/26 , G06K9/0014 , G06K9/036 , G06K9/4647 , G06K9/4652 , G06K9/4661 , H04N5/2256
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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公开(公告)号：US10078778B2
公开(公告)日：2018-09-18
申请号：US14997439
申请日：2016-01-15
IPC分类号： G01N15/14 , G01N35/00 , G02B21/16 , H04N5/225 , G02B21/26 , B01L3/00 , G06K9/00 , G01N33/483 , G06K9/03 , G06K9/46 , G01N15/00 , G01N15/10
CPC分类号： G06K9/00147 , B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/0896 , B01L2300/12 , G01N15/1425 , G01N15/1463 , G01N33/4833 , G01N35/00029 , G01N35/00871 , G01N2015/0065 , G01N2015/1006 , G01N2035/00148 , G02B21/16 , G02B21/26 , G06K9/0014 , G06K9/036 , G06K9/4647 , G06K9/4652 , G06K9/4661 , H04N5/2256
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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公开(公告)号：US20200372241A1
公开(公告)日：2020-11-26
申请号：US16990390
申请日：2020-08-11
IPC分类号： G06K9/00 , B01L3/00 , G01N15/14 , G01N35/00 , G02B21/16 , G02B21/26 , H04N5/225 , G01N33/483 , G06K9/03 , G06K9/46
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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公开(公告)号：US10776608B2
公开(公告)日：2020-09-15
申请号：US16102118
申请日：2018-08-13
IPC分类号： G06K9/00 , B01L3/00 , G01N15/14 , G01N35/00 , G02B21/16 , G02B21/26 , H04N5/225 , G01N33/483 , G06K9/03 , G06K9/46 , G01N15/00 , G01N15/10
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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公开(公告)号：US09953209B2
公开(公告)日：2018-04-24
申请号：US15085175
申请日：2016-03-30
IPC分类号： G06K9/00 , B01L3/00 , G01N15/14 , G01N35/00 , G02B21/16 , G02B21/26 , H04N5/225 , G01N33/483 , G06K9/03 , G06K9/46 , G01N15/00 , G01N15/10
CPC分类号： G06K9/00147 , B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/0896 , B01L2300/12 , G01N15/1425 , G01N15/1463 , G01N33/4833 , G01N35/00029 , G01N35/00871 , G01N2015/0065 , G01N2015/1006 , G01N2035/00148 , G02B21/16 , G02B21/26 , G06K9/0014 , G06K9/036 , G06K9/4647 , G06K9/4652 , G06K9/4661 , H04N5/2256
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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公开(公告)号：US20160217315A1
公开(公告)日：2016-07-28
申请号：US15085175
申请日：2016-03-30
IPC分类号： G06K9/00 , G06K9/46 , G06K9/03 , G01N33/483
CPC分类号： G06K9/00147 , B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/0896 , B01L2300/12 , G01N15/1425 , G01N15/1463 , G01N33/4833 , G01N35/00029 , G01N35/00871 , G01N2015/0065 , G01N2015/1006 , G01N2035/00148 , G02B21/16 , G02B21/26 , G06K9/0014 , G06K9/036 , G06K9/4647 , G06K9/4652 , G06K9/4661 , H04N5/2256
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
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9.发明申请SYSTEMS, METHODS, AND APPARATUS FOR IN VITRO SINGLE-CELL IDENTIFICATION AND RECOVERY 审中-公开体外单细胞识别和恢复的系统，方法和装置公开(公告)号：US20160209319A1
公开(公告)日：2016-07-21
申请号：US14997439
申请日：2016-01-15
CPC分类号： G06K9/00147 , B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/0896 , B01L2300/12 , G01N15/1425 , G01N15/1463 , G01N33/4833 , G01N35/00029 , G01N35/00871 , G01N2015/0065 , G01N2015/1006 , G01N2035/00148 , G02B21/16 , G02B21/26 , G06K9/0014 , G06K9/036 , G06K9/4647 , G06K9/4652 , G06K9/4661 , H04N5/2256
摘要： Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
摘要翻译： 本文描述了用于从生物物质样品（例如生物流体）自动识别和回收感兴趣的个体细胞的系统，方法和装置。 还描述了丰富感兴趣的细胞类型的方法。 这些系统，方法和装置允许以下两个或多个以下协调的性能，例如全部具有相同的装置，从而实现高吞吐量：细胞富集，细胞鉴定和单个细胞恢复用于进一步分析（例如，测序） 的个体回收细胞。
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