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    System for real time detection of nucleic acid amplification products
    1.
    发明授权
    System for real time detection of nucleic acid amplification products 失效
    核酸扩增产物实时检测系统

    公开(公告)号：US5928907A

    公开(公告)日：1999-07-27

    申请号：US752973

    申请日：1996-12-02

    申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

    CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

    摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

    摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。

    Method and apparatus for quantitating surface-binding optical resonance profiles
    8.
    发明授权
    Method and apparatus for quantitating surface-binding optical resonance profiles 有权
    用于定量表面结合光学共振谱的方法和装置

    公开(公告)号：US08060328B2

    公开(公告)日：2011-11-15

    申请号：US12881403

    申请日：2010-09-14

    申请人： David H. Tracy

    发明人： David H. Tracy

    IPC分类号： G01R9/00 ,  G01R9/04 ,  G01C7/00 ,  G01F25/00

    CPC分类号： G01N21/274 ,  G01N21/553 ,  G01N21/648

    摘要： An apparatus for quantitation of surface-binding optical resonance profiles includes a calibration module including a calibration scan result fetcher, a calibration profile creation module, and a fitting module. The fitting module includes an experimental scan result fetcher, a calibration profile fetcher, and a resonance shift determination module. A method for qualifying a surface plasmon resonance chip is also described herein.

    摘要翻译： 用于定量表面结合光学共振分布的装置包括校准模块，其包括校准扫描结果读取器，校准曲线创建模块和拟合模块。 配件模块包括实验扫描结果提取器，校准配置文件取出器和谐振位移确定模块。 本文还描述了用于限定表面等离子体共振芯片的方法。

    Standardizing a spectrometric instrument
    9.
    发明授权
    Standardizing a spectrometric instrument 失效
    标准化光谱仪

    公开(公告)号：US6049762A

    公开(公告)日：2000-04-11

    申请号：US993482

    申请日：1997-12-18

    申请人： Alan M. Ganz ,  Yongdong Wang ,  David H. Tracy ,  Robert A. Hoult ,  Jerry E. Cahill ,  David A. Huppler

    发明人： Alan M. Ganz ,  Yongdong Wang ,  David H. Tracy ,  Robert A. Hoult ,  Jerry E. Cahill ,  David A. Huppler

    IPC分类号： G01J3/28 ,  G01J3/453 ,  G01R35/00

    CPC分类号： G01J3/453 ,  G01J2003/2866

    摘要： Standardization is achieved for FTIR spectrometric instruments that effect an intrinsic distortion in spectral information, the distortion being associated with an aperture size. An idealized function of spectral line shape is specified. With a small calibration aperture, spectral data is obtained for a basic sample having known "true" spectral data, and standard spectral data also is obtained for a standard sample. With a larger, normal sized aperture, standard spectral data is obtained again for the calibration sample. A transformation factor, that is a function of this data and the standardized function, is applied to spectral data for test samples to effect standardized information. In another embodiment, the standard sample has known true spectral data, and the basic sample is omitted. In either case, the transformation factor is applied to the sample data in logarithm form, the antilogarithm of the result effects the standardized information.

    摘要翻译： 对于影响光谱信息中的固有失真的FTIR光谱仪器实现标准化，失真与孔径尺寸相关联。 规定了光谱线形状的理想化功能。 使用小的校准孔径，获得具有已知“真实”光谱数据的基本样品的光谱数据，并且还为标准样品获得标准光谱数据。 对于较大的正常尺寸的孔径，再次为校准样品获得标准光谱数据。 作为该数据和标准化函数的函数的变换因子被应用于测试样本的光谱数据以实现标准化信息。 在另一个实施例中，标准样品具有已知的真实光谱数据，并省略了基本样品。 在任一种情况下，转换因子以对数形式应用于样本数据，结果的反对数效应影响标准化信息。

    Method and apparatus for comparing spectra
    10.
    发明授权
    Method and apparatus for comparing spectra 失效
    比较光谱的方法和装置

    公开(公告)号：US5308982A

    公开(公告)日：1994-05-03

    申请号：US956519

    申请日：1992-10-05

    申请人： Juan Ivaldi ,  David H. Tracy ,  Robert Hoult ,  Richard Spragg

    发明人： Juan Ivaldi ,  David H. Tracy ,  Robert Hoult ,  Richard Spragg

    IPC分类号： G01N21/27 ,  G01J3/28 ,  G01J3/433 ,  G01N21/73 ,  G01N21/25

    CPC分类号： G01J3/433 ,  G01J3/28

    摘要： A method for determining concentration of an analyte in a sample includes generating a spectrum of a selected analyte and interferents, generating a spectrum of an unknown sample, computing first and second derivatives of the sample spectrum, deriving a matrix model that includes the analyte spectrum and the derivatives, and applying the matrix model to the sample spectrum so as to yield a parameter representing concentration of the selected analyte in the unknown sample. Multiple linear least squares regression is utilized to fit the model and calculate the parameter.

    摘要翻译： 用于确定样品中分析物浓度的方法包括产生所选分析物和干扰物的光谱，产生未知样品的光谱，计算样品光谱的第一和第二导数，导出包括分析物光谱的矩阵模型，以及 衍生物，并将矩阵模型应用于样本光谱，以产生表示未知样品中所选分析物浓度的参数。 利用多元线性最小二乘回归拟合模型并计算参数。