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    System for real time detection of nucleic acid amplification products
    1.
    发明授权
    System for real time detection of nucleic acid amplification products 失效
    核酸扩增产物实时检测系统

    公开(公告)号：US5928907A

    公开(公告)日：1999-07-27

    申请号：US752973

    申请日：1996-12-02

    申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

    CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

    摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

    摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。

    Apparatus for a fluid impingement thermal cycler
    3.
    发明授权
    Apparatus for a fluid impingement thermal cycler 失效
    用于流体冲击热循环仪的装置

    公开(公告)号：US5942432A

    公开(公告)日：1999-08-24

    申请号：US946512

    申请日：1997-10-07

    申请人： Douglas H. Smith ,  John Shigeura ,  Timothy M. Woudenberg

    发明人： Douglas H. Smith ,  John Shigeura ,  Timothy M. Woudenberg

    IPC分类号： C12N15/09 ,  B01L7/00 ,  C12M1/00 ,  C12M1/18 ,  C12Q1/68 ,  C12M3/00

    CPC分类号： B01L7/52

    摘要： Apparatus are disclosed that thermally cycles samples between at least two temperatures. These apparatus operate by impinging fluid jets onto the outer walls of a sample containing region. Because the impinging fluid jets provide a high heat transfer coefficient between the jet and the sample containing region, the sample containing regions are uniformly cycled between the two temperatures. The heat exchange rate between the jets and the sample regions are substantially uniform.

    摘要翻译： 公开了在至少两个温度之间热循环样品的装置。 这些装置通过将流体射流冲击到样品容纳区域的外壁上来操作。 由于冲击流体射流在射流和样品含有区域之间提供高的传热系数，所以含有区域的样品在两个温度之间均匀循环。 射流和样品区之间的热交换率基本上是均匀的。

    Fluid Manipulation Assembly
    4.
    发明申请
    Fluid Manipulation Assembly 审中-公开
    流体操纵装置

    公开(公告)号：US20090258415A1

    公开(公告)日：2009-10-15

    申请号：US12490219

    申请日：2009-06-23

    申请人： Zbigniew T. Bryning ,  John Shigeura

    发明人： Zbigniew T. Bryning ,  John Shigeura

    IPC分类号： C12M1/34

    CPC分类号： B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683

    摘要： Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a new communication results in the assembly between the cover layer and the substrate and/or so that a new barrier wall is formed. Systems for carrying out the methods are also provided.

    摘要翻译： 提供微流体组件，系统和方法来操作流体样品。 组件包括可弹性变形的覆盖层和较少可弹性变形的衬底。 所述方法包括通过覆盖层使基底变形，使得当覆盖层反弹新的通信导致覆盖层和基底之间的组装和/或使得形成新的阻挡壁时。 还提供了执行方法的系统。

    Sample handling system for a multi-channel capillary electrophoresis device
    6.
    发明授权
    Sample handling system for a multi-channel capillary electrophoresis device 有权
    多通道毛细管电泳装置的样品处理系统

    公开(公告)号：US07169279B2

    公开(公告)日：2007-01-30

    申请号：US10288405

    申请日：2002-11-04

    申请人： Howard Gregg King ,  John Shigeura ,  Eric S. Nordman ,  Sean Matthew Desmond

    发明人： Howard Gregg King ,  John Shigeura ,  Eric S. Nordman ,  Sean Matthew Desmond

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44743

    摘要： A sample handling system in a multi-channel capillary electrophoresis apparatus is disclosed. The sample handling system includes a work surface for supporting a plurality of samples located at a plurality of work surface coordinates and a sample loading assembly comprising a plurality of loading wells. At least one of the loading wells includes a capillary fixedly positioned therein. The system further includes a programmable sample transfer device for automatically transferring a sample from a work surface coordinate to a loading well. The invention further includes methods for using the sample handling system.

    摘要翻译： 公开了一种多通道毛细管电泳装置中的样品处理系统。 样品处理系统包括用于支撑位于多个工作表面坐标处的多个样品的工作表面和包括多个加载孔的样品加载组件。 至少一个装载井包括固定地定位在其中的毛细管。 该系统还包括用于将样品从工作面坐标自动转移到装载井的可编程样品转移装置。 本发明还包括使用样品处理系统的方法。

    Time-delay integration in a flow cytometry system
    8.
    发明申请
    Time-delay integration in a flow cytometry system 有权
    流式细胞术系统中的延时整合

    公开(公告)号：US20080094627A1

    公开(公告)日：2008-04-24

    申请号：US11644412

    申请日：2006-12-22

    申请人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura ,  Janice Shigeura

    发明人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura ,  Janice Shigeura

    IPC分类号： G01N21/00

    CPC分类号： G01N27/44721 ,  B82Y10/00 ,  B82Y20/00 ,  G01N2015/149 ,  G01N2015/1497

    摘要： An apparatus for detecting analytes in a sample is provided. The apparatus can include: a flow cytometry system including one or more detection zones; one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from detectable analytes in the one or more detection zones, which have been excited by the radiation, wherein at least one detector includes an output; a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector; and a sorting mechanism, for example, to effect flow cytometry. Methods for detecting analytes in a sample are also provided.

    摘要翻译： 提供了一种用于检测样品中分析物的装置。 该装置可以包括：流式细胞术系统，其包括一个或多个检测区域; 一个或多个辐射源被设置用于用辐射照射一个或多个检测区域; 至少一个检测器，其被设置用于收集对应于已被所述辐射激发的所述一个或多个检测区域中的可检测分析物发射的光信号的电荷，其中至少一个检测器包括输出; 耦合到所述至少一个检测器的系统，用于通过在读取所述至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分; 以及分选机制，例如进行流式细胞术。 还提供了检测样品中分析物的方法。

    Electrophoretic system with multi-notch filter and laser excitation source
    9.
    发明申请
    Electrophoretic system with multi-notch filter and laser excitation source 有权
    具有多陷波滤波器和激光源的电泳系统

    公开(公告)号：US20080043235A1

    公开(公告)日：2008-02-21

    申请号：US11894829

    申请日：2007-08-22

    申请人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura ,  Janice Shigeura

    发明人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura ,  Janice Shigeura

    IPC分类号： G01N21/00

    CPC分类号： G01N21/6456 ,  B82Y10/00 ,  B82Y20/00 ,  G01N27/44721 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6471 ,  G01N2201/0628

    摘要： Apparatus and methods for detecting analytes in a sample are provided. The apparatus can include: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with radiation; a detector capable of collecting at least one charge corresponding to an emission beam emitted from the detection zone, the detector having an output; and a time delay integration system coupled to the detector for effecting time delay integration of the at least one charge by accumulating the at least one charge before reading the at least one charge at the output of the detector. Various embodiments provide an apparatus with a multi-notch filter. Various embodiments provide a solid state laser, a micro-wire laser, or an organic light-emitting diode as a irradiation source.

    摘要翻译： 提供了用于检测样品中分析物的装置和方法。 该装置可以包括：具有检测区域的一个或多个通道; 设置用于辐射照射检测区域的一个或多个照射源; 检测器，其能够收集对应于从所述检测区域发射的发射光束的至少一个电荷，所述检测器具有输出; 以及耦合到所述检测器的时间延迟积分系统，用于通过在所述检测器的输出读取所述至少一个电荷之前积累所述至少一个电荷来实现所述至少一个电荷的时间延迟积分。 各种实施例提供具有多陷波滤波器的装置。 各种实施例提供固态激光器，微线激光器或有机发光二极管作为照射源。