利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.022 秒)

    Electroactive materials for stimulation of biological activity of stem cells
    1.
    发明授权
    Electroactive materials for stimulation of biological activity of stem cells 失效

    公开(公告)号：US06569654B2

    公开(公告)日：2003-05-27

    申请号：US09753407

    申请日：2001-01-02

    申请人： Venkatram Shastri ,  Ivan Martin ,  Robert Langer ,  Nahid Rahman

    发明人： Venkatram Shastri ,  Ivan Martin ,  Robert Langer ,  Nahid Rahman

    IPC分类号： C12N1300

    CPC分类号： A61K41/0052 ,  C12M35/02 ,  C12N5/0068 ,  C12N13/00 ,  C12N2533/30 ,  Y10S977/91 ,  Y10S977/925

    摘要： Compositions, methods and systems are provided for the stimulation of biological activities within stem cells by applying electromagnetic stimulation to an electroactive material, wherein the electromagnetic stimulation is coupled to the electromagnetic material. In general the present invention involves attaching or associating the desired cells to or with a surface comprising an electroactive material, and applying electromagnetic radiation directly to the desired area. In preferred embodiments, the stimulation of biological activities within cells results from inducing one or more activities including, but not limited to, gene expression, cell growth, cell differentiation, signal transduction, membrane permeability, cell division, contraction, and cell signaling. In exemplary embodiments, the electroactive materials used in the present invention are either two-dimensional substrates such as thin films having at least one surface of an electroactive material, or in alternative embodiments, the electroactive materials are three-dimensional substrates comprising a matrix having at least one surface of an electroactive material.

    Electroactive materials for stimulation of biological activity of bone marrow stromal cells
    2.
    发明授权
    Electroactive materials for stimulation of biological activity of bone marrow stromal cells 失效
    用于刺激骨髓基质细胞生物活性的电活性材料

    公开(公告)号：US06190893B1

    公开(公告)日：2001-02-20

    申请号：US09156317

    申请日：1998-09-18

    申请人： Venkatram R. Shastri ,  Nahid Rahman ,  Ivan Martin ,  Robert S. Langer, Jr.

    发明人： Venkatram R. Shastri ,  Nahid Rahman ,  Ivan Martin ,  Robert S. Langer, Jr.

    IPC分类号： C12N1300

    CPC分类号： C12N13/00 ,  C12M35/02 ,  C12N5/0068 ,  C12N2533/30

    摘要： Compositions, methods and systems are provided for the stimulation of biological activities within bone marrow stromal cells by applying electromagnetic stimulation to an electroactive material, wherein the electromagnetic stimulation is coupled to the electromagnetic material. In general the present invention involves attaching or associating the desired bone marrow stromal cells to or with a surface comprising an electroactive material, and applying electromagnetic radiation directly to the desired area. In preferred embodiments, the stimulation of biological activities within bone marrow stromal cells results from inducing one or more activities including, but not limited to, gene expression, cell growth, cell differentiation, signal transduction, membrane permeability, cell division and cell signalling. In particularly preferred embodiments, the present invention stimulates bone cell regeneration. In exemplary embodiments, the electroactive materials used in the present invention are either two-dimensional substrates such as thin films having at least one surface of an electroactive material, or in alternative embodiments, the electroactive materials are three-dimensional substrates comprising a matrix having at least one surface of an electroactive material.

    摘要翻译： 提供了组合物，方法和系统，用于通过对电活性材料施加电磁刺激来刺激骨髓基质细胞内的生物活性，其中电磁刺激与电磁材料耦合。 通常，本发明涉及将期望的骨髓基质细胞附着或连接到包含电活性物质的表面或与包含电活性材料的表面相连，并将电磁辐射直接施加到所需区域。 在优选的实施方案中，刺激骨髓基质细胞内的生物活性是由于诱导一种或多种活性，包括但不限于基因表达，细胞生长，细胞分化，信号转导，膜通透性，细胞分裂和细胞信号传导。 在特别优选的实施方案中，本发明刺激骨细胞再生。 在示例性实施方案中，本发明中使用的电活性材料是二维底物，例如具有至少一种电活性材料表面的薄膜，或者在替代实施方案中，电活性材料是三维基质，其包含基质， 电活性材料的至少一个表面。

    Use of fibroblast growth factor 2 for expansion of chondrocytes and tissue engineering
    3.
    发明授权
    Use of fibroblast growth factor 2 for expansion of chondrocytes and tissue engineering 失效
    使用成纤维细胞生长因子2扩展软骨细胞和组织工程

    公开(公告)号：US06582960B1

    公开(公告)日：2003-06-24

    申请号：US09397417

    申请日：1999-09-17

    申请人： Ivan Martin ,  Lisa E. Freed ,  Robert Langer ,  Gordana Vunjak-Novakovic

    发明人： Ivan Martin ,  Lisa E. Freed ,  Robert Langer ,  Gordana Vunjak-Novakovic

    IPC分类号： C12N500

    CPC分类号： C12N5/0655 ,  A61K35/12 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2510/00

    摘要： The present invention provides an improved method for expanding cells for use in tissue engineering. In particular the method provides specific biochemical factors to supplement cell culture medium during the expansion process in order to reproduce events occurring during embryonic development with the goal of regenerating tissue equivalents that resemble natural tissues both structurally and functionally. These specific biochemical factors improve proliferation of the cells and are capable of de-differentiation mature cells isolated from tissue so that the differentiation potential of the cells is preserved. The bioactive molecules also maintain the responsiveness of the cells to other bioactive molecules. Specifically, the invention provides methods for expanding chondrocytes in the presence of fibroblast growth factor 2 for use in regeneration of cartilage tissue.

    摘要翻译： 本发明提供了用于组织工程中用于扩张细胞的改进方法。 特别地，该方法提供特定的生物化学因子以在扩增过程中补充细胞培养基，以便再现胚胎发育期间发生的事件，目的是在结构和功能上再生类似于天然组织的组织等同物。 这些具体的生物化学因素改善了细胞的增殖，并且能够使从组织中分离的成熟细胞去分化，从而保持了细胞的分化潜能。 生物活性分子还保持细胞对其他生物活性分子的反应性。 具体地说，本发明提供了在成纤维细胞生长因子2存在下扩展软骨细胞用于软骨组织再生的方法。

    Bioengineered anterior cruciate ligament
    7.
    发明授权
    Bioengineered anterior cruciate ligament 有权

    公开(公告)号：US06287340B1

    公开(公告)日：2001-09-11

    申请号：US09312203

    申请日：1999-05-14

    申请人： Gregory Altman ,  David Kaplan ,  Gordana Vunjak-Novakovic ,  Ivan Martin

    发明人： Gregory Altman ,  David Kaplan ,  Gordana Vunjak-Novakovic ,  Ivan Martin

    IPC分类号： A61F208

    CPC分类号： C12M35/04 ,  A61F2/08 ,  A61L27/3604 ,  A61L27/3691 ,  A61L27/38 ,  A61L2430/10 ,  C12M21/08 ,  C12M25/14 ,  Y10S623/901

    摘要： The present invention provides a method for producing an anterior cruciate ligament ex vivo. The method comprises seeding pluripotent stem cells in a three dimensional matrix, anchoring the seeded matrix by attachment to two anchors, and culturing the cells within the matrix under conditions appropriate for cell growth and regeneration, while subjecting the matrix to one or more mechanical forces via movement of one or both of the attached anchors. Bone marrow stromal cells are preferably used as the pluripotent cells in the method. Suitable matrix materials are materials to which cells can adhere, such as a gel made from collagen type I. Suitable anchor materials are materials to which the matrix can attach, such as Goinopra coral and also demineralized bone. Optimally, the mechanical forces to which the matrix is subjected mimic mechanical stimuli experienced by an anterior cruciate ligament in vivo. This is accomplished by delivering the appropriate combination of tension, compression, torsion, and shear, to the matrix. The bioengineered ligament which is produced by this method is characterized by a cellular orientation and/or matrix crimp pattern in the direction of the applied mechanical forces, and also by the production of collagen type I, collagen type III, and fibronectin proteins along the axis of mechanical load produced by the mechanical forces. Optimally, the ligament produced has fiber bundles which are arranged into a helical organization. The method for producing an anterior cruciate ligament can be adapted to produce a wide range of tissue types ex vivo by adapting the anchor size and attachment sites to reflect the size of the specific type of tissue to be produced, and also adapting the specific combination of forces applied, to mimic the mechanical stimuli experienced in vivo by the specific type of tissue to be produced. The methods of the present invention can be further modified to incorporate other stimuli experienced in vivo by the particular developing tissue, some examples of the stimuli being chemical stimuli, and electro-magnetic stimuli. Some examples of tissue which can be produced include other ligaments in the body (hand, wrist, elbow, knee), tendon, cartilage, bone, muscle, and blood vessels.

    Bioreactor for Tissue Engineering
    8.
    发明申请
    Bioreactor for Tissue Engineering 审中-公开
    组织工程生物反应器

    公开(公告)号：US20080311650A1

    公开(公告)日：2008-12-18

    申请号：US11568727

    申请日：2005-05-04

    申请人： Marcel Jakob ,  Karl Jakob ,  Ivan Martin ,  Nicholas Eion Timmins

    发明人： Marcel Jakob ,  Karl Jakob ,  Ivan Martin ,  Nicholas Eion Timmins

    IPC分类号： C12N5/06 ,  C12M1/00

    CPC分类号： C12M25/14 ,  C12M27/14 ,  C12M29/10

    摘要： The present invention relates to a method and apparatus for growing cells in a three-dimensional scaffold. Relative movement of the scaffold and an end cap of the culture chamber results in circulation of the growth medium through the scaffold. The invention is also suited for introduction of cells into a scaffold. The scaffold may be any sort of natural or synthetic material that will support cellular life, including a tissue.

    摘要翻译： 本发明涉及用于在三维支架中生长细胞的方法和装置。 支架和培养室的端盖的相对运动导致生长培养基循环通过支架。 本发明也适用于将细胞引入支架。 支架可以是支持细胞生命的任何种类的天然或合成材料，包括组织。