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公开(公告)号：US07842780B2
公开(公告)日：2010-11-30
申请号：US10541182
申请日：2004-01-07
CPC分类号： A61K38/1825 , A61K38/1841 , A61K47/42 , A61L27/227 , A61L27/26 , A61L27/3604 , A61L27/3847 , A61L27/3852 , A61L27/3895 , A61L27/56 , D01D5/00 , D01F4/02 , Y10T428/29 , C08L89/00
摘要： The present invention provides processes for producing porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffolds described herein can be adjusted as to mimic the gradient of densities found in natural tissue. Accordingly, methods for engineering of 3-dimensional tissue, e.g. bone and cartilage, using the silk fibroin scaffold material are also provided.
摘要翻译： 本发明提供生产多孔丝素蛋白支架材料的方法。 多孔丝素蛋白支架可用于组织工程。 可以调节本文所述的丝素蛋白支架的孔隙率以模拟在天然组织中发现的密度梯度。 因此，用于三维组织工程的方法，例如， 还提供使用丝素蛋白骨架材料的骨和软骨。
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2.发明申请公开(公告)号：US20080274157A1
公开(公告)日：2008-11-06
申请号：US12079629
申请日：2008-03-26
CPC分类号： A61K35/32 , A61B17/00491 , A61F2/30744 , A61F2/30756 , A61F2/3859 , A61F2002/2817 , A61F2002/2839 , A61F2002/2867 , A61F2002/30062 , A61F2002/30224 , A61F2002/30225 , A61F2002/30759 , A61F2210/0004 , A61F2230/0069 , A61F2310/00365 , A61F2310/00383 , A61K38/00 , A61K38/363 , A61K38/4833 , A61L24/0005 , A61L24/0015 , A61L27/3608 , A61L27/3612 , A61L27/3654 , A61L27/3687 , A61L27/38 , A61L27/48 , A61L27/54 , A61L27/56 , A61L2300/414 , A61L2300/43 , A61L2300/64 , A61L2430/06 , A61K2300/00
摘要： The invention is directed toward a cartilage repair assembly comprising a shaped structure of subchondral bone with an integral overlying cartilage cap which is treated to remove cellular debris and proteoglycans and milled cartilage in a bioabsorbable carrier. The shaped structure is dimensioned to fit in a drilled bore in a cartilage defect area so that said shaped bone and cartilage cap when centered in the bore does not engage the side wall of the bore and is positioned from the side wall of the bone a distance ranging from 10 microns to 1000 microns and is surrounded by milled cartilage and a fibrin thrombin glue. A method for inserting the assembly into a cartilage defect area is disclosed.
摘要翻译： 本发明涉及一种软骨修复组件，其包括具有整体上覆软骨帽的软骨下骨的成形结构，其被处理以除去生物可吸收载体中的细胞碎片和蛋白聚糖和研磨的软骨。 该成形结构的尺寸适于装配在软骨缺损区域中的钻孔中，使得当在孔中心定位时所述成形的骨和软骨帽不接合孔的侧壁并且从骨的侧壁定位一定距离 范围从10微米到1000微米，被碾磨的软骨和纤维蛋白凝血酶胶包围。 公开了一种将组件插入软骨缺损区域的方法。
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3.发明申请Covalently immobilized protein gradients in three-dimensional porous scaffolds 有权在三维多孔支架中共价固定的蛋白质梯度公开(公告)号：US20070212730A1
公开(公告)日：2007-09-13
申请号：US11407373
申请日：2006-04-19
摘要： The invention provides a method for forming an immobilized agent gradient within a 3-dimensional porous scaffold. A 3-dimensional scaffold formed from a biocompatible material is provided. The surface of the scaffold and/ or the agent is activated so as to allow binding of the agent to the scaffold. The activated scaffold is contacted with a solution containing the agent. Contact with the solution is maintained for a sufficient period of time to allow diffusion of the solution through a portion of the scaffold, thereby forming a desired gradient of the agent through the 3-dimensional scaffold.
摘要翻译： 本发明提供了在三维多孔支架内形成固定化剂梯度的方法。 提供由生物相容性材料形成的3维支架。 支架和/或试剂的表面被活化，以允许试剂与支架结合。 活化的支架与含有该试剂的溶液接触。 保持与溶液接触足够的时间以允许溶液扩散通过支架的一部分，从而通过3维支架形成所需试剂的梯度。
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公开(公告)号：US20060273279A1
公开(公告)日：2006-12-07
申请号：US10541182
申请日：2004-01-07
IPC分类号： A23J7/00
CPC分类号： A61K38/1825 , A61K38/1841 , A61K47/42 , A61L27/227 , A61L27/26 , A61L27/3604 , A61L27/3847 , A61L27/3852 , A61L27/3895 , A61L27/56 , D01D5/00 , D01F4/02 , Y10T428/29 , C08L89/00
摘要： The present invention provides processes for producing porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffolds described herein can be adjusted to mimic the gradient of densities found in natural tissue. Accordingly, methods for engineering of 3-dimensional tissue, e.g. bone and cartilage, using the silk fibroin scaffold material are also provided.
摘要翻译： 本发明提供生产多孔丝素蛋白支架材料的方法。 多孔丝素蛋白支架可用于组织工程。 可以调节本文所述的丝素蛋白支架的孔隙率以模拟在天然组织中发现的密度梯度。 因此，用于三维组织工程的方法，例如， 还提供使用丝素蛋白骨架材料的骨和软骨。
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公开(公告)号：US09958436B2
公开(公告)日：2018-05-01
申请号：US14126902
申请日：2012-06-15
CPC分类号： G01N33/5091 , C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for determining cell chirality using micropatterned substrates are disclosed. Also provided are methods for diagnosing diseases such as genetic diseases or cancer by comparing the chirality of sample cells from a subject with normal cells, and determining a difference in chirality between the sample cells and normal cells.
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6.发明授权Application of electrical stimulation for functional tissue engineering in vitro and in vivo 有权电刺激在体外和体内功能组织工程中的应用公开(公告)号：US08367410B2
公开(公告)日：2013-02-05
申请号：US10872577
申请日：2004-06-21
CPC分类号： C12N5/0658 , C12N2502/02 , C12N2502/1358 , C12N2529/00
摘要： The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.
摘要翻译： 本发明提供了体外制备生物人造组织当量的新方法及其在体内植入后的增强整合。 这些方法包括在体外培养过程中将组织构建物递送至仿生电刺激，以改善其结构和功能性质，和/或在植入该构建体后体内，以增强其与宿主组织的整合并增加细胞存活和功能。 本发明的方法特别可用于生产生物人造等同物和/或修复和替换含有电可兴奋细胞的天然组织，并且在体内受到电刺激，例如心肌组织，条纹骨骼肌组织 ，平滑肌肉组织，骨骼，脉管系统和神经组织。
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7.发明申请Propagation of undifferentiated embryonic stem cells in hyaluronic acid hydrogel 审中-公开未分化胚胎干细胞在透明质酸水凝胶中的传播公开(公告)号：US20070122392A1
公开(公告)日：2007-05-31
申请号：US11473870
申请日：2006-06-22
CPC分类号： C12N5/0606 , C12N2501/115 , C12N2501/905 , C12N2502/13 , C12N2533/80
摘要： Embryonic stem cells are propagated in a hyaluronic acid.
摘要翻译： 胚胎干细胞在透明质酸中繁殖。
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8.发明授权Use of fibroblast growth factor 2 for expansion of chondrocytes and tissue engineering 失效使用成纤维细胞生长因子2扩展软骨细胞和组织工程公开(公告)号：US06582960B1
公开(公告)日：2003-06-24
申请号：US09397417
申请日：1999-09-17
IPC分类号： C12N500
CPC分类号： C12N5/0655 , A61K35/12 , C12N2500/25 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/15 , C12N2501/33 , C12N2510/00
摘要： The present invention provides an improved method for expanding cells for use in tissue engineering. In particular the method provides specific biochemical factors to supplement cell culture medium during the expansion process in order to reproduce events occurring during embryonic development with the goal of regenerating tissue equivalents that resemble natural tissues both structurally and functionally. These specific biochemical factors improve proliferation of the cells and are capable of de-differentiation mature cells isolated from tissue so that the differentiation potential of the cells is preserved. The bioactive molecules also maintain the responsiveness of the cells to other bioactive molecules. Specifically, the invention provides methods for expanding chondrocytes in the presence of fibroblast growth factor 2 for use in regeneration of cartilage tissue.
摘要翻译： 本发明提供了用于组织工程中用于扩张细胞的改进方法。 特别地，该方法提供特定的生物化学因子以在扩增过程中补充细胞培养基，以便再现胚胎发育期间发生的事件，目的是在结构和功能上再生类似于天然组织的组织等同物。 这些具体的生物化学因素改善了细胞的增殖，并且能够使从组织中分离的成熟细胞去分化，从而保持了细胞的分化潜能。 生物活性分子还保持细胞对其他生物活性分子的反应性。 具体地说，本发明提供了在成纤维细胞生长因子2存在下扩展软骨细胞用于软骨组织再生的方法。
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公开(公告)号：US09808557B2
公开(公告)日：2017-11-07
申请号：US12672521
申请日：2008-08-11
申请人： Michael L. Lovett , Xianyan Wang , Christopher M. Cannizzaro , Gordana Vunjak-Novakovic , David L. Kaplan
发明人： Michael L. Lovett , Xianyan Wang , Christopher M. Cannizzaro , Gordana Vunjak-Novakovic , David L. Kaplan
CPC分类号： A61L27/227 , A61F2/07 , A61F2/08 , A61F2/82 , A61L27/26 , A61L27/3604 , D10B2211/22 , Y10T428/13 , C08L89/00
摘要： Described are tubular silk fibroin compositions and methods for their manufacture and use. Tubular compositions as described herein can be produced in a range of high burst strengths, can easily be made in a range of inner diameters, can be derivatized with functional moieties, and can be produced in a range of permeabilities suitable for particularized uses. In one aspect, the tubular compositions can be used in the repair or replacement of damaged or diseased blood vessels, including, but not limited to vessels smaller than about 6 mm.
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公开(公告)号：US20110076384A1
公开(公告)日：2011-03-31
申请号：US12934666
申请日：2009-04-08
摘要： A system and method for making a biomaterial device includes a support structure providing a shape for a biomaterial device. At least one applicator has a supply of biomaterial solution and is positioned along the support structure. The at least one applicator forms a biomaterial fiber by applying shear force to the biomaterial solution and delivering the biomaterial fiber to the support structure. A controller causes relative movement between the support structure and the at least one applicator, and the biomaterial fiber is arranged on the support structure according to the relative movement to form the biomaterial device. The biomaterial may be silk fibroin which may be wound onto a reciprocating and rotating mandrel. Control over the properties of the biomaterial device is achieved through appropriate selection of material processing, winding strategy, and post-winding processing.
摘要翻译： 用于制造生物材料装置的系统和方法包括为生物材料装置提供形状的支撑结构。 至少一个施用器具有生物材料溶液的供应并且沿着支撑结构定位。 所述至少一个施用器通过向所述生物材料溶液施加剪切力并将所述生物材料纤维递送到所述支撑结构来形成生物材料纤维。 控制器引起支撑结构和至少一个施加器之间的相对运动，并且根据相对运动将生物材料纤维布置在支撑结构上以形成生物材料装置。 生物材料可以是可以卷绕到往复和旋转的心轴上的丝素蛋白。 通过适当选择材料加工，卷绕策略和后卷绕处理，可以控制生物材料装置的性能。
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